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Inhibitor, inhibitor composition, medicament and use thereof

A technology of inhibitors and drugs, applied in the field of inhibitors and inhibitor compositions, can solve the problem that the function of the zkscan3 gene is poorly understood, etc.

Active Publication Date: 2022-05-17
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the function of the zkscan3 gene under normal physiological conditions

Method used

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  • Inhibitor, inhibitor composition, medicament and use thereof
  • Inhibitor, inhibitor composition, medicament and use thereof
  • Inhibitor, inhibitor composition, medicament and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] See figure 1 , the present embodiment adopts the following steps to carry out:

[0044] 1) The percentages of erythrocyte subsets in bone marrow and spleen of zkscan3 knockout mice and wild mice were detected by flow cytometry. See Figure 2A-Figure 2F, (2B-2E) The percentages of red blood cells in mouse spleen and bone marrow were analyzed by flow cytometry and statistically analyzed (n=10). (2F) Detection of RBC, reticulocytes, and MCHC in the peripheral blood of mice by the five-differential hematology analyzer. The results showed an increased percentage of early and late erythroblasts in the spleen of zkscan3 knockout mice. Decreased percentage of early erythroblasts in bone marrow. The other subpopulations of red blood cells were not significantly different in peripheral blood.

[0045] 2) The red blood cells in the spleen and bone marrow were detected by flow cytometry, and the peripheral blood of mice was detected by a five-differential blood analyzer. Spec...

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PUM

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Abstract

The invention relates to an inhibitor, an inhibitor composition, a drug and applications thereof, revealing that zkscan3 interacts with GATA1 and inhibits the transcriptional activity of GATA1, thereby affecting erythropoiesis. At the same time, it was also revealed that the zkscan3 gene can lead to erythrocyte denucleation disorder by promoting the expression of KLF1. Based on this, the inhibitor of the present invention can affect the production and maturation of red blood cells by regulating the transcription of GATA1 and KLF1.

Description

technical field [0001] The invention relates to an inhibitor, an inhibitor composition, a medicine and an application thereof, and belongs to the technical field of biomedicine. Background technique [0002] ZKSCAN3 is a zinc finger protein with KRAB and SCAN domains, belonging to the Krüppel-associated box domain zinc finger protein (KRAB-ZFP) family, which is involved in a variety of cell life activities, such as cell proliferation, apoptosis and tumorigenesis. biological transformation, etc. There are four subfamilies of KRAB-ZFPs, two with KRAB and C2H2 zinc finger motifs and two with an additional SCAN at the N-terminus (SCAN-ZFP). The KRAB domain has the function of repressing gene transcription, while the C2H2 zinc finger domain binds to specific DNA sequences, and the function of the SCAN domain is still poorly understood. It has been reported that ZKSCAN3 promotes the proliferation, migration and invasion of cancer cells by upregulating the expression of genes rel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P7/00
CPCA61K45/00A61P7/00C07K16/18A61K39/3955
Inventor 杨林李自宣
Owner SUZHOU UNIV