Screening method of silicate bacteria with high potassium dissolving capacity suitable for low-temperature subacid environment and potassium-dissolving bacterial fertilizer

Abstract

The invention discloses a screening method of silicate bacteria with high potassium dissolving capacity suitable for a low-temperature subacid environment. The method mainly comprises the following steps: separating silicate bacteria with potassium dissolving capacity by using a selective culture medium through a dilution inverted plate method and a streak method; a shake-flask fermentation test determines the potassium dissolving capacity of the separated silicate bacteria, strains with strong potassium dissolving capacity are selected, fungus chaff is used as a carrier, a compost maturity method is adopted to prepare the potassium bacterial fertilizer, and water spinach is used as a material to evaluate the toxicity and fertilizer efficiency of the potassium bacterial fertilizer. According to the invention, separated silicate bacteria are subjected to a potassium dissolving capability test; the results show that part of strains still have high potassium dissolving capacity at low temperature, a seed germination index test shows that the potassium-dissolving bacterial fertilizer prepared by taking mushroom bran as a carrier is non-toxic to seeds, and indexes such as plant height,leaf area and the like of water spinach in a bacterial fertilizer application group are obviously higher than those of a control group. The bacterial strain screened by the method is high in potassiumdissolving capacity, strong in stress resistance and suitable for preparing microbial fertilizer.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, and is especially suitable for the screening of silicate bacteria with high potassium-dissolving ability in a low-temperature slightly acidic environment and potassium-dissolving bacterial fertilizer. Background technique [0002] The soil itself is a natural potassium pool, but more than 95% of the potassium exists in the form of silicate minerals such as potassium feldspar and mica, which have low solubility and are insoluble, making it difficult for plants to use. This creates a contradiction that the soil is both rich in potassium and deficient in potassium. [0003] Potassium-solubilizing bacteria can decompose fixed potassium in the soil and release available potassium that can be absorbed and utilized by crops. Potassium-soluble bacteria include Bacillus extorquens, Bacillus mucilaginosus, Bacillus circulans and the like. [0004] Bacillus colloidus, also known as silicate ...

AI Technical Summary

Problems solved by technology

As the most commonly used carrier of microbial fertilizer, peat has disadvantages such as long regeneration period, great damage to the environment when mining, and relatively expensive price. Finding cheap and high-quality carriers is a key issue to be considered in the production of microbial fertilizers

Mushroom chaff is the leftovers from the production of edible fungi, which used to be directly discarded or burned, which not only causes waste of resources, but also leads to the breeding of mold and pests, increasing the number of harmful spores and pests in the air, thus causing environmental pollution

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