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Efficient potassium-dissolving nitrogen-fixing bacterium and application thereof

A nitrogen-fixing bacteria and potassium-dissolving technology, applied in the field of microorganisms, can solve problems such as inability to apply, low soil survival rate, and unsatisfactory bacterial efficacy.

Active Publication Date: 2022-05-13
FENGTIANBAO AGRI TECHOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the existing literature reports, although there have been reports on nitrogen-fixing bacteria and potassium-decomposing bacteria, the efficacy of the bacteria is not satisfactory, and there are few bacteria that have both potassium-solving and nitrogen-fixing functions. The rate is not high and cannot be applied to actual agricultural production

Method used

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  • Efficient potassium-dissolving nitrogen-fixing bacterium and application thereof
  • Efficient potassium-dissolving nitrogen-fixing bacterium and application thereof
  • Efficient potassium-dissolving nitrogen-fixing bacterium and application thereof

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preparation example Construction

[0031] In the present invention, the preparation method of Bacillus tequila FTB-K2 fermentation liquid preferably includes the following steps: after activating the high-efficiency potassium-solubilizing nitrogen-fixing bacteria of the present invention, inoculate it in the fermentation medium, cultivate it at 28-32°C for 20- 28h.

[0032] In the present invention, the time for activating Bacillus tequila FTB-K2 is preferably 22-26h, more preferably 23-25h. The activation medium used for the activation of Bacillus tequila FTB-K2 strain preferably includes the following components in parts by weight: 8-12 parts of peptone, 3-7 parts of beef extract powder, and 3-7 parts of sodium chloride , 10-14 parts of agar, 800-1200 parts of water, pH7.2-7.4, more preferably include the following components in parts by weight: 9-11 parts of peptone, 4-6 parts of beef extract powder, sodium chloride 4-6 parts, 11-13 parts of agar, 900-1100 parts of water, pH 7.3. The present invention has ...

Embodiment 1

[0037] Determination of Potassium Solubilizing Ability of Bacillus Tequila FTB-K2

[0038] Inoculate Bacillus tequila FTB-K2 in 8g of peptone, 3g of beef extract powder, 3g of sodium chloride, 10g of agar, 800g of water, and an activation medium of pH 7.2, activate for 22 hours, and then inoculate into 2g of corn steep liquor powder, beef Extract powder 4g, tryptone 3g, compound inorganic salt (KH 2 PO 4 :K 2 HPO 4 : MnSO 4 ·H 2 O (2:1:1) 0.5g, water 1000g, pH value 6.8 fermentation medium, 28°C, 90r / min culture for 20h, to obtain bacterial suspension.

[0039] Preparation of potassium solution medium (without water-soluble potassium ions): glucose 5g, ammonium sulfate 0.5g, yeast powder 0.5g, magnesium sulfate 0.3g, disodium hydrogen phosphate 2g, ferrous sulfate 0.03g, manganese sulfate 0.03g, potassium long 2 g of stone (potassium content 11%-13%), 1 L of distilled water, stir evenly, adjust the pH value to 7.2, and sterilize at 121° C. for 15 minutes.

[0040] 500mL...

Embodiment 2

[0044] Determination of Nitrogen Fixation Ability of Bacillus Tequila FTB-K2

[0045] Preparation of nitrogen-free medium: by weight, the medium consists of the following components: glucose 10g, potassium hydrogen phosphate 0.2g, magnesium sulfate heptahydrate 0.2g, sodium chloride 0.2g, calcium carbonate 5g, calcium sulfate 0.1g 1,000ml of distilled water; adjust the pH of the culture medium to 72, and sterilize at 121°C for 30 minutes for later use;

[0046] Bacillus tequila FTB-K2 was inoculated in the activation medium with 8 g of peptone, 3 g of beef extract powder, 3 g of sodium chloride, 800 g of water, pH 7.2, and activated for 22 hours. Experimental group: Take 5ml of the activated Bacillus tequila FTB-K2 suspension into a 250mL Erlenmeyer flask containing 100ml of nitrogen-free liquid medium, shake and culture at 30°C and 80r / min for 30d, repeat 15 flasks. The control group was not inoculated with bacterial suspension. At 2, 4, 6, 8, and 10 days after inoculation,...

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Abstract

The invention provides efficient potassium-dissolving nitrogen-fixing bacteria and application thereof, and belongs to the technical field of microorganisms. The efficient potassium-dissolving nitrogen-fixing bacterium provided by the invention is named as bacillus tequilensis in taxonomy, has the strain number of FTB-K2, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.23347. The bacillus tequilensis FTB-K2 separated from the soil has high potassium dissolving capacity and good nitrogen fixation capacity, the content of available potassium and available nitrogen in the soil can be increased, and the utilization efficiency of insoluble potassium in the soil is improved; the fertilizer can decompose insoluble aluminosilicate inorganic mineral substances such as potassium feldspar, promote insoluble nutrient elements such as potassium, silicon and magnesium to be converted into soluble nutrients, and can be used as a microbial fertilizer to increase the content of available nutrients in soil, promote the growth and development of crops and increase the yield. The survival rate in soil is high, and the method can be applied to actual agricultural production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a high-efficiency potassium-decomposing nitrogen-fixing bacterium and its application. Background technique [0002] Nitrogen and potassium are essential elements for plant growth, and they are indispensable. Nitrogen plays a very important role in crop growth. It is a component of amino acids in plants, a component of protein, and a component of chlorophyll that plays a decisive role in photosynthesis in plants. Nitrogen also helps crops reproduce. Therefore, nitrogen can not only increase the yield of agricultural products, but also improve the quality of agricultural products. Potassium in plants generally accounts for 0.2%-4.1% of dry matter weight, second only to nitrogen. Potassium participates in the activation of more than 60 enzyme systems, photosynthesis, transport of assimilated products, carbohydrate metabolism and protein synthesis during plant growth...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05G3/00C12R1/07
CPCC12N1/20C05F11/08C05G3/00Y02W30/40
Inventor 陈月锋陈小飞陈铁成张丽英陆媛玉
Owner FENGTIANBAO AGRI TECHOLOGY CO LTD
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