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Multifunctional potassium-dissolving bacteria and use thereof

A multi-functional, potassium-solubilizing bacteria technology, applied in the field of microorganisms, can solve the problems of low survival rate, few strains, unevenness, etc., and achieves broad application prospects, simple operation, and the effect of reducing the amount of use.

Inactive Publication Date: 2018-07-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the existing literature reports, the species of Burkholderia, phosphate-solubilizing and nitrogen-fixing bacteria are diverse and uneven, and there are very few strains that have the functions of dissolving potassium, dissolving phosphorus and fixing nitrogen. Few, the survival rate in the soil is not high, there are few types of strains used in actual production, and there is a lack of innovation

Method used

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  • Multifunctional potassium-dissolving bacteria and use thereof
  • Multifunctional potassium-dissolving bacteria and use thereof
  • Multifunctional potassium-dissolving bacteria and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the screening of multifunctional potassium-solubilizing bacteria

[0018] The screening method of the multifunctional potassium decomposing bacteria of the present invention has the following steps:

[0019] Step 1. Take 10g of corn rhizosphere soil from the corn field of Huazhong Agricultural University, put it into a triangular flask with glass beads, add 90mL of sterile water, shake at a constant temperature of 28-30°C for 30-45min, and disperse the sample evenly in a sterile water, resulting in a dilution factor of 10 -1 The bacterial suspension, the supernatant was taken after the bacterial suspension was allowed to stand, and diluted successively to obtain 10 -3 、10 -5 、10 -7 Bacteria suspension sample with double concentration gradient, 10 -3 、10 -5 、10 -7 Take 0.1ml of each bacterial suspension with a double concentration gradient, spread it evenly on the Alexander Borov solid medium in sequence, put it in a constant temperature incubator, an...

Embodiment 2

[0024] Embodiment 2: the identification of multifunctional potassium-solubilizing bacteria

[0025] A bacterial strain numbered as H1 obtained above was identified as Burkholderia tripica (Burkholderia tripica) H1 through morphological identification, biochemical identification and 16S rRNA sequencing analysis;

[0026] 1. The results of colony morphology are as attached figure 1 , the growth physiological form of the bacteria is that the surface of the colony is smooth, light yellow, with a transparent membrane on the surface, neat edges, and a round convex shape;

[0027] 2. The result of biochemical identification is Gram-negative bacteria, rod-shaped bacteria with motility and aerobic properties;

[0028] 3. Use primers 27F and 1492R to amplify and forward sequence the strain H1, and obtain a sequence length of 1403bp. The specific sequence is as follows:

[0029]

[0030] The sequence was compared with the sequence in GenBank database by BLAST, and its similarity with ...

Embodiment 3

[0031] Implementation 3: Determination of Potassium Solubilizing Ability of Multifunctional Potassium Solubilizing Bacteria

[0032] Prepare potassium-solubilizing medium (without water-soluble potassium ions) in 95 mL, add 0.5 g of accurately weighed potassium ore powder into a 250 mL Erlenmeyer flask, sterilize at 121°C for 30 min, and sterilize the Burkholderia The H1 suspension was inoculated into the strains in the shake flask with 5% inoculum amount, and three parallels were performed, and no inoculation control was set at the same time. After 30 days of culture on a water bath shaker at 37°C, stop the culture, take 5ml of the fermentation broth, add 1ml of 6% H 2 o 2 , digested in a boiling water bath for 1h, centrifuged the digested solution at 5000r / min for 10min, took the supernatant and measured K on a flame photometer + concentration. The potassium-decomposing effect of Burkholderia H1 is as follows: figure 2 shown by figure 2 It can be seen that the highest...

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Abstract

The invention provides multifunctional potassium-dissolving bacteria. The multifunctional potassium-dissolving bacteria have a taxonomic name of Burkholderia tripica H1 and have a preservation numberof CCTCC NO. M2017281. The invention also provides a use of the multifunctional potassium-dissolving bacteria in soil potassium dissolving, phosphorus solubilization and nitrogen fixation. The invention also provides a microbial agent for dissolving potassium and phosphorus in soil and fixing nitrogen. The multifunctional potassium-dissolving bacteria are fermented into a fermentation liquid and the fermentation liquid is spray-dried to form microbial dry powder. The multifunctional potassium-dissolving bacteria can effectively dissolve potassium and phosphorus and fix nitrogen in the soil, increase the content of available nutrients in the soil, reduce the use of various fertilizers, increase a crop yield, reduce a water and soil loss, has an important significance and application value in culture and production of soil ecological fertility and keeping of the agricultural ecological balance, realizes a low production cost, is easy to operate, has no pollution and does not damage the environment.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a multifunctional potassium-dissolving bacterium and its function in dissolving potassium, dissolving phosphorus and fixing nitrogen in soil, and a bacterial agent for dissolving potassium, dissolving phosphorus and fixing nitrogen in soil. Background technique [0002] Nitrogen, phosphorus, and potassium are three important nutrients necessary for crop growth. Nitrogen is a component of protein, nucleic acid and chlorophyll in crops. Phosphorus plays an important role in increasing crop yield and improving crop quality. Potassium can improve the ability of crops to adapt to adverse external environments. However, the total nitrogen content of the soil in most of the cultivated land in my country is below 0.2% (i.e., nitrogen-deficient soil), and the area of ​​phosphorus-deficient soil is as high as 1.009 billion mu, and the directly usable potassium content in the soil only account...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K17/16C12R1/01C09K101/00
CPCC09K17/16C09K2101/00C12N1/205C12R2001/01
Inventor 肖乃东周洋周文兵朱端卫胡金龙蔡建波
Owner HUAZHONG AGRI UNIV
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