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Potassium-dissolving growth-promoting Burkholderia pyrrocinia and microbial inoculum and application of Burkholderia pyrrocinia

A Burkholderia pyrrole and potassium-dissolving technology, applied in the field of microorganisms, can solve problems such as increased drug resistance, water pollution, hardening, etc., and achieve the effects of increasing the potassium content of tobacco leaves, increasing the potassium content of leaves, and promoting plant growth

Pending Publication Date: 2022-05-24
LUZHOU CO LTD SICHUAN TOBACCO +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive application of potassium fertilizer not only increases the cost of tobacco cultivation, but also seriously affects the ecological environment of the planting area, such as increased resistance to pests and diseases, soil compaction, excessive heavy metal content, water pollution, etc., leading to environmental pollution and waste of resources

Method used

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  • Potassium-dissolving growth-promoting Burkholderia pyrrocinia and microbial inoculum and application of Burkholderia pyrrocinia
  • Potassium-dissolving growth-promoting Burkholderia pyrrocinia and microbial inoculum and application of Burkholderia pyrrocinia
  • Potassium-dissolving growth-promoting Burkholderia pyrrocinia and microbial inoculum and application of Burkholderia pyrrocinia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Isolation and screening of strain DY211

[0044] Soil samples were collected from the tobacco rhizosphere soil in the tobacco-growing area of ​​Luzhou, Sichuan, and different microorganisms were isolated from the soil samples. The morphological characteristics of the colonies were observed day by day, and the strains with transparent potassium-solubilizing circles or plaques were selected, purified by plate streaking, and the purified strains were stored on solid potassium-solubilizing medium for later use.

[0045] The above-mentioned strains to be used are cultured in a shaker flask, activated and propagated, a potassium-solubilizing solid medium is prepared, and a sterilized plate is prepared. After the medium has been sterilized, the plate is poured. , seal the plate and place it at 28°C for culture, and observe the experimental results when there is a transparent potassium solution or plaque. combine figure 1 and figure 2, select the strain DY211 with...

Embodiment 2

[0049] Example 2 Determination of Potassium Solubilization Ability of Strain DY211

[0050] The medium was prepared according to the formula of the seed liquid medium, and the medium was divided into 250 mL conical flasks, 100 mL per bottle. Under aseptic conditions, inoculate the activated strains into the seed liquid medium with an inoculation loop, inoculate one loop per bottle, repeat three times, wrap the bottle with aseptic sealing film, and cultivate in a shaker at 28°C, aseptically every 24 hours. Operate sampling, count the number of bacteria in the fermentation broth, and wait for the number of viable bacteria to increase to 10 8 When it is about cfu / mL, it can be used as seed solution. Sterilize one bottle of seed solution at 121°C for 20 min to prepare inactivated seed solution for use.

[0051] The medium was prepared according to the fermentation medium formula, which was divided into 250 mL conical flasks, 100 mL per bottle, the bottle mouth was wrapped with a...

Embodiment 3

[0055] Example 3 Identification of strain DY211

[0056] 1. Morphological identification

[0057] like figure 1 As shown, the Gram staining results showed that strain DY211 was Gram-negative and spherical. The colonies are round, transparent and slightly whitish, with a moist and shiny surface, regular edges, and bulges like droplets.

[0058] 2. Physiological and biochemical identification

[0059] Referring to the "Common Bacterial System Identification Manual", the potassium-solubilizing bacteria strains were initially identified through physiological and biochemical tests. The identification results showed: Gram-negative, indole reaction negative, V-P test negative, methyl red reaction positive, production Hydrogen sulfide was negative, oxidase test was negative, indole test was negative, and rhamnose, glucose, and sucrose utilization tests were positive.

[0060] According to the above identification results, the potassium-solubilizing bacteria strain DY211 was prelim...

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Abstract

The invention discloses potassium-dissolving growth-promoting burkholderia pyrrocinia as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. Wherein the Burkholderia pyrrocinia DY211 is preserved in the China Center for Type Culture Collection on November 17, 2021, and the preservation registration number of the Burkholderia pyrrocinia DY211 is CCTCC (China Center for Type Culture Collection) M20211436. The invention also provides a Burkholderia pyrrocinia microbial agent and a preparation method thereof. The Burkholderia pyrrocinia microbial agent can be used for dissolving potassium and promoting plant growth. The bacterial liquid prepared from the DY211 can effectively improve the biomass of tobacco plants, the stem height of the tobacco plants in the mature period is increased by 12.0%, the leaf number is increased by 12.9%, the stem diameter is increased by 16.9%, the middle leaf length is increased by 28.8%, and the middle leaf width is increased by 18.5%. The bacterial liquid prepared from the DY211 can effectively improve the potassium content of leaves at the top and in the middle of the tobacco, the quality of the tobacco is improved, the use of chemical fertilizers is reduced, and the potassium-dissolving growth-promoting bacterial strain and the bacterial liquid thereof have wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a potassium-solution-promoting Burkholderia pyrrole and its bacterial agent and application. Background technique [0002] Tobacco (Nicotiana tabacum) belongs to the Solanaceae annual herb and is one of the important economic crops. my country's tobacco production accounts for 1 / 3 of the world's total, and it has extensive planting areas in many regions. Tobacco is not only an important part of my country's national economy, but also a special industry with high taxation. At present, it has become an important pillar of local finance in Henan, Yunnan, Sichuan and other regions of my country. Potassium is in high demand during tobacco growth. As one of the quality elements of flue-cured tobacco, potassium is closely related to the combustion completeness, combustion uniformity, taste, aroma, chemical composition and safety of tobacco leaves, and plays a signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02A01N63/20A01P21/00C12R1/01
CPCC12N1/20C12N1/02A01N63/20Y02A40/10
Inventor 周本国谢强蔡悦顾勇阮班锋夏春唐小飞张永辉王飞张远盖崔权仁许大凤
Owner LUZHOU CO LTD SICHUAN TOBACCO
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