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A kind of multifunctional potassium decomposing bacteria and its application

A multifunctional, potassium-dissolving bacteria technology, applied in the field of microorganisms, can solve the problems of low survival rate, few types of strains, lack of innovation, etc., achieve broad application prospects, simple operation, and reduce soil erosion

Inactive Publication Date: 2021-04-06
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the existing literature reports, the species of Burkholderia, phosphate-solubilizing and nitrogen-fixing bacteria are diverse and uneven, and there are very few strains that have the functions of dissolving potassium, dissolving phosphorus and fixing nitrogen. Few, the survival rate in the soil is not high, there are few types of strains used in actual production, and there is a lack of innovation

Method used

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  • A kind of multifunctional potassium decomposing bacteria and its application
  • A kind of multifunctional potassium decomposing bacteria and its application
  • A kind of multifunctional potassium decomposing bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the screening of multifunctional potassium-solubilizing bacteria

[0018] The screening method of the multifunctional potassium decomposing bacteria of the present invention has the following steps:

[0019] Step 1. Take 10g of corn rhizosphere soil from the corn field of Huazhong Agricultural University, put it into a triangular flask with glass beads, add 90mL of sterile water, shake at a constant temperature of 28-30°C for 30-45min, and disperse the sample evenly in a sterile water, resulting in a dilution factor of 10 -1 The bacterial suspension, the supernatant was taken after the bacterial suspension was allowed to stand, and diluted successively to obtain 10 -3 、10 -5 、10 -7 Bacteria suspension sample with double concentration gradient, 10 -3 、10 -5 、10 -7 Take 0.1ml of each bacterial suspension with a double concentration gradient, spread it evenly on the Alexander Borov solid medium in sequence, put it in a constant temperature incubator, an...

Embodiment 2

[0024] Embodiment 2: the identification of multifunctional potassium-solubilizing bacteria

[0025] A bacterial strain numbered as H1 obtained above was identified as Burkholderia tripica (Burkholderia tripica) H1 through morphological identification, biochemical identification and 16S rRNA sequencing analysis;

[0026] 1. The results of colony morphology are as attached figure 1 , the growth physiological form of the bacteria is that the surface of the colony is smooth, light yellow, with a transparent membrane on the surface, neat edges, and a round convex shape;

[0027] 2. The result of biochemical identification is Gram-negative bacteria, rod-shaped bacteria with motility and aerobic properties;

[0028] 3. Using primers 27F and 1492R to amplify and forward-sequence strain H1, the obtained sequence length is 1403bp, and the specific sequence is as follows:

[0029]

[0030] The sequence was compared with the sequence in GenBank database by BLAST, and its similarity wit...

Embodiment 3

[0031] Implementation 3: Determination of Potassium Solubilizing Ability of Multifunctional Potassium Solubilizing Bacteria

[0032] Prepare potassium-solubilizing medium (without water-soluble potassium ions) in 95 mL, add 0.5 g of accurately weighed potassium ore powder into a 250 mL Erlenmeyer flask, sterilize at 121°C for 30 min, and sterilize the Burkholderia The H1 suspension was inoculated into the strains in the shake flask with 5% inoculum amount, and three parallels were performed, and no inoculation control was set at the same time. After 30 days of culture on a water bath shaker at 37°C, stop the culture, take 5ml of the fermentation broth, add 1ml of 6% H 2 o 2 , digested in a boiling water bath for 1h, centrifuged the digested solution at 5000r / min for 10min, took the supernatant and measured K on a flame photometer + concentration. The potassium-decomposing effect of Burkholderia H1 is as follows: figure 2 shown by figure 2 It can be seen that the highest...

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Abstract

The invention provides a multifunctional potassium-solubilizing bacterium, which is taxonomically named Burkholderia tripica (Burkholderia tripica) H1, and whose preservation number is: CCTCC NO.M2017281. The invention also provides the application of the multifunctional potassium-solubilizing bacterium in soil potassium-dissolving, phosphorus-dissolving and nitrogen-fixing. The present invention also provides a soil potassium-dissolving, phosphorus-dissolving and nitrogen-fixing bacterial agent, which is a dry powder bacterial agent obtained by fermenting the above-mentioned multifunctional potassium-solubilizing bacteria to obtain a fermented liquid and spray drying. The above-mentioned multifunctional potassium-dissolving bacteria can effectively decompose potassium, dissolve phosphorus and fix nitrogen to the soil. After application, the content of available nutrients in the soil can be increased, the use of various fertilizers can be reduced, the yield of crops can be increased, and soil erosion can be reduced. It is of great significance and application value in terms of exerting soil ecological fertility and maintaining agricultural ecological balance, and has low cost, simple operation, no pollution, and no harm to the environment.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a multifunctional potassium-dissolving bacterium and its function in dissolving potassium, dissolving phosphorus and fixing nitrogen in soil, and a bacterial agent for dissolving potassium, dissolving phosphorus and fixing nitrogen in soil. Background technique [0002] Nitrogen, phosphorus, and potassium are three important nutrients necessary for crop growth. Nitrogen is a component of protein, nucleic acid and chlorophyll in crops. Phosphorus plays an important role in increasing crop yield and improving crop quality. Potassium can improve the ability of crops to adapt to adverse external environments. However, the total nitrogen content of the soil in most of the cultivated land in my country is below 0.2% (i.e., nitrogen-deficient soil), and the area of ​​phosphorus-deficient soil is as high as 1.009 billion mu, and the directly usable potassium content in the soil only account...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/16C12R1/01C09K101/00
CPCC09K17/16C09K2101/00C12N1/205C12R2001/01
Inventor 肖乃东周洋周文兵朱端卫胡金龙蔡建波
Owner HUAZHONG AGRI UNIV
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