Application of lectin probe combination in identification of rat sex based on urine protein glycotype

A technology of lectin and urine protein, which is applied in the direction of general structure detail detection of gas analyzers, material inspection products, measuring devices, etc., can solve the problems of not getting enough complete and clear sugar chain distribution information, and achieve low cost Effect

Pending Publication Date: 2020-10-09
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Studies have found that the sugar chains in human serum, saliva, and urine are different between men and women, but there is still not enough complete and clear sugar chain distribution information

Method used

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  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype
  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype
  • Application of lectin probe combination in identification of rat sex based on urine protein glycotype

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Embodiment Construction

[0022] The relevant experiments and analysis of the present application are described in detail below, and the inventor's specific research and development process is not limited thereto.

[0023] 1. Experimental part

[0024] 1.1 Reagents and materials

[0025] Glycine, disodium hydrogen phosphate, sodium dihydrogen phosphate, Tween-20 and protease inhibitors were all purchased from Sigma-Aldrich, USA. Bovine serum albumin (BSA) was purchased from Merck, Germany. Cy3 fluorescence was purchased from American Amerhsma Company. Other chemical reagents were of analytical grade and were not further purified before use. All experimental water was ultrapure water treated by Milli-Q50 pure water system (Millipore, USA). Sephadex G-25 column desalting was purchased from GE Healthcare, USA. Chip hybridization kit was purchased from Bio-Rad, USA. Other common glassware are all made in China. Thirty-seven lectins (see Table 1 for specific names) were purchased from Sigma-Aldrich a...

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Abstract

The invention provides an application of a lectin probe combination in identification of rat sex based on urine protein glycotype. The specific lectin probe combination is a combination with significant difference of AAL, DSA, Jacalin, EEL and PTL-II, and when a sample detection result shows that the expression of AAL, DSA and Jacalin is up-regulated and the expression of EEL and PTL-II is down-regulated, it shows that a corresponding main body is a male rat; otherwise, the rat is a female rat. The method for identifying the sex of the rat is quick, simple, convenient and accurate, and the implementation cost is low.

Description

technical field [0001] The invention relates to a method for identifying the sex of rats. Background technique [0002] In order to improve the productivity of domestic animals, sex control of mammals has become an important content in animal husbandry research. People often hope to produce animals of the expected sex, thereby promoting the advancement of animal genetics and breeding technology, strengthening the prevention and treatment of associated diseases, and better utilization of resources. thereby serving humanity itself. In the study of wild animals, gender is one of the basic contents of animal population ecology research, and the individual ratio of male and female animals plays an important role in revealing the population structure. [0003] With the development of molecular genetics, developmental biology and related disciplines, early sex identification methods include: X-correlation enzyme identification, chromosome karyotype analysis, H-Y antigen detection ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N33/533
CPCG01N33/68G01N33/543G01N33/533G01N2440/38
Inventor 郭松涛常鸿莉陈瑞白瑞峰祝丰何刚李保国
Owner NORTHWEST UNIV(CN)
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