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Method for recovering riboflavin by combining nanofiltration concentration with flocculation method

A technology of riboflavin and flocculation, which is applied in the biological field and can solve the problem of high equipment requirements

Active Publication Date: 2020-10-23
BEIJING POLYTECHNIC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production of riboflavin by microbial fermentation will produce a large amount of riboflavin crystallization mother liquor. The riboflavin in these crystallization mother liquors cannot be collected and treated as waste liquid. Finally, it is proposed to use macroporous adsorption resin to reclaim riboflavin, but This method needs to use high-concentration hydrochloric acid for desorption, and high-concentration hydrochloric acid will be limited by equipment and other conditions, and the requirements for actual production equipment are relatively high

Method used

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  • Method for recovering riboflavin by combining nanofiltration concentration with flocculation method
  • Method for recovering riboflavin by combining nanofiltration concentration with flocculation method
  • Method for recovering riboflavin by combining nanofiltration concentration with flocculation method

Examples

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Embodiment 1

[0026] This embodiment provides a method for recovering riboflavin by nanofiltration concentration combined with flocculation, comprising the following steps:

[0027] (1) Firstly, LNF 8272697 roll-type nanofiltration membrane was used to treat riboflavin crystallization mother liquor by nanofiltration, in which the temperature was controlled at 40°C, the pH was 2, and the pressure was 30bar to obtain the retentate and filtrate; the rejection rate was 99.98 %, the average flux is 105.47 L m-2 h -1;

[0028] The riboflavin crystallization mother liquor is the waste liquid produced during the separation and purification process of riboflavin fermentation broth, and the concentration of riboflavin in it was determined to be 0.5mg / mL;

[0029] (2) Add flocculant—anionic 63810 type (14 million molecular weight) polyacrylamide to the retentate, under stirring conditions, temperature 20°C, pH 3, the obtained flocculant is centrifuged, and the obtained precipitate is For riboflavin p...

Embodiment 2

[0035] In order to study the influence of roll-type nanofiltration membrane operating conditions on the retention performance of riboflavin solution, this example conducts experiments by adjusting the pH and operating pressure of riboflavin mother liquor.

[0036] The operating temperature, pressure and crystallization mother liquor have certain influence on the nanofiltration operation. For the experimental nanofiltration separation equipment——BSM-NFZ-01, only the operating pressure and the riboflavin mother liquor in the sampling tank can be controlled manually. pH, while riboflavin mother liquor is acidic, for nanofiltration, its pressure range is generally between 15--30bar, therefore, the present embodiment has studied the pH (2,3,4,5 ) and operating pressure (15, 20, 25, 30 bar) on the interception effect.

[0037] The results are shown in Table 1:

[0038]

[0039] The data in Table 1 shows that the rejection rate of the roll nanofiltration membrane LNF 8272697 to t...

Embodiment 3

[0041] In order to study the influence of different roll-type nanofiltration membrane cleaning methods on the roll-type membrane, this example studies the roll-type membrane acid-base cleaning scheme, sets up multiple variables for comparison, and studies the operating pressure (2, 4, 6bar), The influence of cleaning time (5, 10 min), cleaning solution temperature (30, 40 ℃) on cleaning results. With the operating pressure at 5 bar and deionized water as the filtrate, measure the water permeation volume within 10 minutes to calculate the average water flux, and use its pure water flux to evaluate the cleaning effect.

[0042] The obtained data are shown in Table 2:

[0043]

[0044] From the comparison of the data in Table 2, it can be seen that:

[0045] (1) The effect of alkali washing is better than that of pickling, because the solubility of riboflavin in alkaline solution is much higher than that in acidic solution;

[0046] (2) Prolonging the cleaning time did not i...

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Abstract

The invention discloses a method for recovering riboflavin by combining nanofiltration concentration with flocculation method, which is used for recovering riboflavin from riboflavin crystallization mother liquor which is waste liquor generated in a riboflavin fermentation liquor separation and purification process by combining nanofiltration concentration with flocculation precipitation method. The method comprises the following steps: performing nanofiltration treatment on the riboflavin crystallization mother liquor by adopting an LNF type roll-type nanofiltration membrane, adding a flocculating agent into an obtained trapped fluid, and performing centrifugal treatment on an obtained flocculating fluid under a stirring condition to obtain a precipitate, namely the riboflavin product. According to the method, the retention rate of the LNF type roll-type nanofiltration membrane on riboflavin can reach 99%, polyacrylamide is selected as the flocculating agent under the optimal condition, and about 90% of riboflavin in trapped fluid can be settled. Through the method provided by the invention, the riboflavin yield is effectively improved, the process and cost of sewage discharge treatment are reduced, the sewage discharge amount is reduced, and clean production is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for recovering riboflavin by nanofiltration concentration combined with flocculation. Background technique [0002] Riboflavin (vitamin B2), is an important part of the vitamin B family, its predecessor is vitamin G, the molecular formula is C 17 h 20 N 4 o 6 , named 7,8-dimethyl-10(1'-D-ribitolyl)-isoalloxazine according to its chemical structure, is a water-soluble vitamin prepared by microbial fermentation or chemical synthesis , It is widely used as a medicine, pigment, nutrition and animal feed additive. [0003] The production of riboflavin by microbial fermentation will produce a large amount of riboflavin crystallization mother liquor. The riboflavin in these crystallization mother liquors cannot be collected and treated as waste liquid. Finally, it is proposed to use macroporous adsorption resin to reclaim riboflavin, but This method requires the use of high-co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D475/14
CPCC07D475/14
Inventor 陈亮辛秀兰杨军谢国莉梁浩田飞豹
Owner BEIJING POLYTECHNIC
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