High-potential super-hydrophilic polypeptide monolayer film and preparation method and application thereof

A super-hydrophilic, single-layer membrane technology, applied in the field of natural polymers, can solve problems such as limiting the application of polypeptide molecules, and achieve the effects of improving cell adhesion and proliferation, increasing loading capacity, and preventing protein adsorption.

Active Publication Date: 2020-10-30
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Although the use of surfactants to regulate the conformation of polypeptide molecules on the interface is relatively common, but due to the complexity of the structure of natural biological macromolecules, there are few reports on the chemical properties of the surface of polypeptide molecular monolayer membranes, which limits the ability of peptide molecules. molecular application

Method used

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  • High-potential super-hydrophilic polypeptide monolayer film and preparation method and application thereof
  • High-potential super-hydrophilic polypeptide monolayer film and preparation method and application thereof
  • High-potential super-hydrophilic polypeptide monolayer film and preparation method and application thereof

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Embodiment 1

[0059] A method for preparing a polypeptide monolayer film, comprising the following steps:

[0060] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0061] (2) Add surfactant SDS to the above-mentioned collagen polypeptide solution to obtain a collagen polypeptide-SDS mixed solution, the concentration of SDS in the mixed solution is 3.50mmol / L (CAC, at 50°C, the critical aggregation concentration of SDS); Medium stable 6h standby.

[0062] (3) Cut a rectangular titanium sheet with a size of 1cm×1cm×1mm, use metallograp...

Embodiment 2

[0066] A method for preparing a polypeptide monolayer film, comprising the following steps:

[0067] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0068] (2) Add the surfactant SDS to the above collagen polypeptide solution to obtain a collagen polypeptide-SDS mixed solution, the concentration of SDS in the mixed solution is 8.32 (6%wt) mmol / L; stabilize it in a water bath for 6h and set aside.

[0069] (3) Cut a rectangular titanium sheet with a size of 1cm×1cm×1mm, use metallographic sandpaper to polish and polish i...

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Abstract

The invention provides a high-potential super-hydrophilic polypeptide monolayer film and preparation method and application thereof. The polypeptide is composed of polypeptide molecules with the molecular weight of (1.48 + / - 0.2)*10 < 5 >g / mol, the thickness of a single-layer film is 13.8-14.9 nm, the exposure amount of primary amino groups on the surface of the film is 12-14%, the Zeta potentialof the polypeptide single-layer film is -1-5 mV, and the contact angle of the film is 10 + / - 1 degrees. As a surface coating material of a cardiovascular stent, the material is applied to treatment ofcardiovascular diseases, and the super-hydrophilic property can enable a layer of hydration film to be formed on the surface of the material so as to effectively prevent protein adsorption. The surface high potential can improve the cell adhesion, proliferation and differentiation capacity.

Description

technical field [0001] The invention belongs to the field of natural polymers, and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with high surface potential and superhydrophilicity, its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Bio-immobilized coatings are often used in the field of biomimetic scaffolds to solve the problem of carrying biomolecules, drug molecules, synthetic polymers or small organic molecules such as enzymes, lactose, and polydopamine. The loading capacity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/02A61L31/10A61L31/14
CPCA61L31/022A61L31/10A61L31/14A61L2420/02A61L2420/08C08L89/00C08L79/02A61L31/044A61L31/129A61L2400/12
Inventor 许静张震李天铎马慧君班青
Owner QILU UNIV OF TECH
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