The invention discloses a preparation method of a super-hydrophilic anti-fogging oxidized graphene coating. The preparation method comprises the following steps: taking graphene micro powder as a raw material, adopting a wet chemical oxidation method to prepare oxidized graphene dispersion liquid, and carrying out filtration, rinsing, ultrasonic dispersion and centrifugal separation to obtain the super-hydrophilic anti-fogging oxidized graphene coating. In addition, the invention further discloses a method of adopting the coating to prepare a super-hydrophilic anti-fogging oxidized graphene coating layer. The preparation method disclosed by the invention is simple in technology, low in cost, green and environment-friendly, and easy in operating condition control. The obtained oxidized graphene coating is excellent in performance, wide in application range, and capable of being applied to relatively transparent common glass or organic transparent carrier. The prepared super-hydrophilic anti-fogging oxidized graphene coating layer has the excellent characteristics of super-hydrophilicity, anti-fogging property, high strength, long durability, high transmission of light and the like. The preparation method of the super-hydrophilic anti-fogging oxidized graphene coating effectively solves the problem that the existing coating layer is poor in anti-fogging effect and durability, and is favorable to promote application and development of the super-hydrophilic technology.