Streptococcus pneumoniae capsular polysaccharides and immunogenic conjugate thereof

A Streptococcus pneumoniae, immunogenic technology, applied in the direction of bacterial antigen components, antibacterial drugs, drug combinations, etc., can solve the problem of lack of effective prevention or treatment of serotype infection

Pending Publication Date: 2020-11-24
SK BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Despite a steady increase in the incidence of pneumococcal disease caused by S. pneumoniae serotypes 2, 9N, 17F, and / or 20, studies that effectively prevent or treat serotype infection are lacking

Method used

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  • Streptococcus pneumoniae capsular polysaccharides and immunogenic conjugate thereof
  • Streptococcus pneumoniae capsular polysaccharides and immunogenic conjugate thereof
  • Streptococcus pneumoniae capsular polysaccharides and immunogenic conjugate thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0309] Example 1. Preparation of polysaccharide-protein conjugate vaccines derived from serotypes 2, 9N, 17F or 20

[0310] [1. Polysaccharide-protein conjugate derived from Streptococcus pneumoniae serotype 2]

[0311] Preparation of polysaccharide-protein conjugates from Streptococcus pneumoniae serotype 2

[0312] Making master cell banks and preparative cell banks

[0313] S. pneumoniae serotype 2 was obtained from the American Type Culture Collection (ATCC) (strain ATCC 6302). The seed stock was grown for several generations in order to strengthen the strain and remove components of animal origin. Seed vials were frozen (<-70°C) using synthetic glycerol as a cryopreservative. To make cell banks, all cultures were propagated in soy-based media. Prior to freezing, cells are concentrated by centrifugation and spent medium is removed, and the cell pellet is then resuspended in new medium containing a cryopreservative (eg, synthetic glycerol).

[0314] fermentation

[03...

Embodiment 2

[0439] Example 2. Preparation of polyvalent Streptococcus pneumoniae polysaccharide-protein conjugates

[0440] [5. Streptococcus pneumoniae 15-valent polysaccharide-protein conjugate]

[0441] Preparation of 15-valent polysaccharide-protein conjugate of Streptococcus pneumoniae

[0442] A formulation comprising polysaccharide-protein conjugates derived from S. pneumoniae serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 9N, 9V, 14, 18C, 19A, 19F, and 23F each conjugated to CRM197 alone 15-valent conjugate composition (15vPnC).

[0443] For serotypes 2 and 9N, conjugates were prepared by the method described above, and for other serotypes, conjugates were prepared according to the method disclosed in Korean Patent Application No. 2012-0065893.

[0444] Calculate the desired volume of final bulk concentrate based on the batch volume and bulk sugar concentration. The required amounts of 0.85% Sodium Chloride, Polysorbate 80, and Succinate Buffer were added to pre-labeled formulation conta...

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Abstract

The objective of the present invention is to provide: an immunogenic composition comprising a Streptococcus pneumoniae polysaccharides-proteins conjugate, which comprises any one or more capsular polysaccharides selected from the group consisting of serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F and 33F, which are derived from Streptococcuspneumoniae, and one or more carrier proteins conjugated to each of the capsular polysaccharides; and a preparation method therefor. According to one embodiment of the present invention, provided is an immunogenic composition for preventing or treating a pneumococcal infection.

Description

technical field [0001] This application requests Korean Patent Application No. 10-2018-0045245 filed on April 18, 2018, Korean Patent Application No. 10-2018-0045246 filed on April 18, 2018, and Korean Patent Application No. 10-2018-0045246 filed on April 18, 2018. Priority of Korean Patent Application No. 10-2018-0045247 and Korean Patent Application No. 10-2018-0045248 filed on April 18, 2018, and the entire contents disclosed in the specification and drawings of the application are hereby incorporated by reference into this article. [0002] The present invention relates to immunogenic compositions and vaccines for Streptococcus pneumoniae, and more particularly, the present invention relates to immunogenic compositions comprising a capsular polysaccharide-carrier protein conjugate of Streptococcus pneumoniae and vaccines. Background technique [0003] Streptococcus pneumoniae is the main pathogenic bacteria of pneumonia. In addition, Streptococcus pneumoniae causes in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61K39/00C07K14/315A61P31/00
CPCC07K14/3156A61K39/092A61K2039/6037A61P31/04A61P37/04A61P31/00A61K2039/6031A61K47/61C07K14/315
Inventor 金勋咸东洙申珍焕安京俊金成炫
Owner SK BIOSCI CO LTD
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