Human epidemic cerebrospinal meningitis-pneumococcus combined vaccine and preparation method thereof

A pneumococcal and combined vaccine technology, which is applied in the biological field to achieve broad-spectrum bactericidal effect and broad-spectrum immune effect.

Active Publication Date: 2018-04-27
BRAVOVAX +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0022] To sum up, there is currently no combined vaccine that can immunize meningococcus and pneumococcus, which is a blank field, and it is urgent to solve this application demand

Method used

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  • Human epidemic cerebrospinal meningitis-pneumococcus combined vaccine and preparation method thereof
  • Human epidemic cerebrospinal meningitis-pneumococcus combined vaccine and preparation method thereof
  • Human epidemic cerebrospinal meningitis-pneumococcus combined vaccine and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0060] 1. Cloning and prokaryotic expression of ΔfHbp and ΔfHbp-NadA recombinant proteins

[0061] 1. Construction of Pet-ΔfHbp and Pet-ΔfHbp-NadA recombinant plasmids

[0062] fHbp is a membrane surface lipoprotein, and many strains of meningococcus have its gene, and strains that do not carry the gene have also been found. According to the antigenic cross-reactivity and sequence similarity of the whole protein, meningococcal fHbp can be divided into three antigenic variant groups V1-V3. In general, antibodies raised against the fHbp of variant V1 (also known as subfamily B) have bactericidal activity against fHbp-expressing strains from variant V1, but not against fHbp in variants V2 and V3 (also known as subfamily A). Strains expressing fHbp and vice versa. The protein molecule of fHbp contains different combinations of five variable domains (V A ~V E ), each variable segment is derived from subfamily A and subfamily B. We designed the structure of ΔfHbp according to i...

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Abstract

The invention provides a recombinant delta fHbp-NadA fusion protein carrier-based human epidemic cerebrospinal meningitis-pneumococcus combined vaccine and a preparation method thereof. Through a flexible linker peptide chain, two active proteins are fused and expressed, activity of the two active proteins is kept and the active proteins are integrated into a single protein so that industrial production and immunization are controllable, a risk is greatly reduced and more polysaccharide-protein binding sites are provided.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a human meningitis-pneumococcus combined vaccine and a preparation method thereof. Background technique [0002] 1. Pathogenic microorganisms and vaccines [0003] Microorganisms that can cause infectious diseases in humans or animals are called pathogenic microorganisms or pathogenic microorganisms. Infection refers to the process of pathogenic microorganisms invading the body, growing and multiplying in certain parts, and causing a series of pathophysiological processes. When pathogenic microorganisms invade the body, the pathogenic microorganisms interact with the body and change each other's activity and function. Therefore, whether they can cause infectious diseases depends on the pathogenicity or virulence of the pathogenic microorganisms on the one hand, and on the other hand. Depends on the body's resistance, that is, immunity. The ability of pathogenic bacteria to cause i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/09A61K39/095A61K39/116A61K39/385A61P31/04
CPCA61K39/09A61K39/095A61K39/116A61K39/385A61K2039/57A61K2039/6031A61K2039/6068A61K2039/62A61K2039/70C07K14/22C07K2319/00C12N15/62C12N15/70A61K2300/00
Inventor 史晋刘昊智艾智武李津吴克
Owner BRAVOVAX
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