Application of ar-turmerone in preparation of product for preventing and treating ulcerative colitis and regulating intestinal florae

A technique for regulating intestinal flora in ulcerative colitis, applied in the field of medicine, to achieve the effect of reducing the degree of inflammation, reducing inflammatory factors, and adjusting intestinal disorders

Active Publication Date: 2020-11-27
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the role of aromatic turmeric in ulcerative colitis and the regulation of intestinal flora.

Method used

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  • Application of ar-turmerone in preparation of product for preventing and treating ulcerative colitis and regulating intestinal florae
  • Application of ar-turmerone in preparation of product for preventing and treating ulcerative colitis and regulating intestinal florae
  • Application of ar-turmerone in preparation of product for preventing and treating ulcerative colitis and regulating intestinal florae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 The improvement of DSS-induced ulcerative colitis in mice by the length of mouse intestinal colon to cecum detected by aromatic curcumin

[0045] 1. Experimental method

[0046]Embodiment Experimental animals are divided into 7 groups at random, are respectively: normal control group (Con), DSS group, turmeric essential oil group (DSS+turmeric essential oil, J, 2.4mg / kg / day), positive drug group (DSS+mesalazine , M, 40mg / kg / day), low-dose aromatic curcumone group (DSS+aromatic curcumone, FL, 10mg / kg / day), medium-dose aromatic curcumone group (DSS+aromatic curcumone, FM, 20mg / kg / day ) and high-dose aromatic curcumone group (40mg / kg / day) with 5 rats in each group.

[0047] Each group was administered continuously for 2 weeks, and 350 mg / kg was administered orally every day. In the first week of administration, except the normal group, the rest of the groups were free to drink DSS solution for one week to prepare the intestinal ulcer model. On the 8th day, the ...

Embodiment 2

[0050] Example 2 Effect of Aroma Curcumone on DSS-Induced Mice Intestinal Tissue Colonic Mucosal Cell Injury

[0051] 1. Experimental method

[0052] The experimental grouping and dosing regimen of this example are the same as in Example 1. After the administration of the mice, the mice were killed by spinal dislocation, and the complete colon tissues were taken out to make paraffin sections and stained with HE. The operation steps included: fixing the colon tissues Soak in 4% paraformaldehyde solution for 6 hours → dehydration: gradually increase from low-concentration alcohol to high-concentration alcohol, put it in 50%, 70%, 80% and 95% alcohol for 12-24 hours each, 100% alcohol for 2-3 hours, then put in xylene and alcohol for half an hour, and then put in xylene until the specimen is transparent → Wax penetration: move the colon tissue into xylene plus paraffin, and put it in a 60°C incubator After half an hour, move it into paraffin to make the paraffin penetrate into t...

Embodiment 3

[0055] Example 3 Effect of Aroma Curcumone on DSS-induced Inflammatory Response in Mouse Colon Tissue

[0056] 1. Experimental method

[0057] The experimental grouping and dosing scheme of this example are the same as in Example 1 and Example 2. After the administration, the mouse spine was killed, and the complete colonic tissue was taken out to make paraffin sections, and immunohistochemical or immunofluorescent staining was performed on the sections, and then The expression of the inflammatory factor TNF-α in the colonic mucosa was determined by immunohistochemistry or immunofluorescence staining, and the experimental data were expressed as Mean ± SD. All experimental data were statistically analyzed using GraphPad Prism7.04 software. Between parameters were tested by ANOVA, P<0.05 was considered to be statistically different.

[0058] Immunohistochemical staining was performed on tissue paraffin sections to detect the expression level of inflammatory factor TNF-α. The p...

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PUM

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Abstract

The invention belongs to the technical field of medicines, and discloses an application of ar-turmerone in preparation of a product for preventing and treating ulcerative colitis. The first study proves that the ar-turmerone can regulate intestinal florae, inhibit the lesion degree of ulcerative colitis and control an ulcerative colitis reaction, thereby inhibiting NF-[kappa]B p65 cell activation,down-regulating expression of inflammatory factors, relieving colon cell injury caused by subsequent DSS and improving the anti-lesion capability, the recovery capability and the absorption functionof colon tissues; and the ar-turmerone can be used for regulating the human intestinal florae so as to treat or prevent the ulcerative colitis. Besides, the invention provides a new application of thear-turmerone, namely an application of the ar-turmerone in prevention and treatment of the ulcerative colitis, and also provides a new selection scheme for treatment of ulcerative colitis diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine. More specifically, it relates to the application of aromatic turmeric in the preparation of products for preventing and treating ulcerative colitis and regulating intestinal flora. Background technique [0002] The intestinal immune system is known as the largest immune system in the human body. The balance of intestinal flora is crucial to our health. However, modern people's diet and living habits, the abuse of antibiotics, caesarean section, deep Insufficient contact with a variety of microorganisms in the natural environment and so on will affect our intestinal flora. It can be said that in contemporary industrialized society, it is difficult to have a completely healthy intestinal flora. [0003] There are many symptoms of colitis, among which ulcerative colitis is a relatively inflammatory type. Other manifestations include loss of appetite, abdominal distension, nausea, vomiting, and hep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P1/00A61P1/04
CPCA61K31/12A61P1/00A61P1/04Y02A50/30
Inventor 张蓝月李春莲石耀辉迟乐菁潘春杏徐雅妮罗怡杰向洪平
Owner GUANGDONG UNIV OF TECH
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