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Quality detection method of malus toringoides fruits

A quality detection method and detection method technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of not judging the color of the fruit, and achieve the effect of broad application prospects

Pending Publication Date: 2020-11-27
SICHUAN ZHIJIACHENG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method for judging the quality of Eseguo and its slices with wolfberry acid

Method used

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  • Quality detection method of malus toringoides fruits
  • Quality detection method of malus toringoides fruits
  • Quality detection method of malus toringoides fruits

Examples

Experimental program
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Embodiment 1

[0031] The content detection of embodiment 1 Russian fruit decoction pieces

[0032] 1) Preparation of the reference substance solution: take the reference substance of wolfberry acid, accurately weigh it, add water to prepare a solution containing 1 mg of wolfberry acid per 1 ml, and obtain it;

[0033] Precisely draw 0.156ml, 0.312ml, 0.625ml, 1.25ml, 2.5ml, 3.75ml of the reference substance solution, put them in a 5ml measuring bottle respectively, add water to the mark, shake well, and prepare a series of reference substance solutions;

[0034] 2) Preparation of the test solution: take 0.5g of Eseguo decoction pieces fine powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered conical flask, add 25m1 of water precisely, seal it tightly, weigh it, and ultrasonically Process (power 500W, frequency 40kHz) for 45 minutes, let cool, weigh again, make up the lost weight with water, shake well, and filter. Take the continued filtrate to obtain;

[0035...

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Abstract

The invention provides a quality detection method of malus toringoides fruits, which adopts high performance liquid chromatography for detection, and comprises the following operation steps of: (1) preparation of a reference substance solution: taking a Chinese wolfberry acid reference substance, and adding water for dissolving to obtain the reference substance solution; (2) preparation of a testsolution: taking a to-be-detected sample, adding water for extraction, filtering, and taking subsequent filtrate as the test solution; (3) respectively sucking the reference solution and the test solution and injecting the reference solution and the test solution into a liquid chromatograph, wherein the chromatographic conditions are as follows: a chromatographic column uses octadecylsilane chemically bonded silica as a filler; and acetonitrile is used as a mobile phase A and 0.1% phosphoric acid solution is used as a mobile phase B for gradient elution. According to the quality detection method of malus toringoides fruit decoction pieces, the Chinese wolfberry acid is used as a determination index of the malus toringoides fruits and the decoction pieces of the malus toringoides fruits, good biological activity and certain specificity are achieved, a simpler and more effective method is provided for quality control over the malus toringoides fruit decoction pieces, and the method has awide application prospect.

Description

technical field [0001] The invention specifically relates to a method for detecting the quality of Russian fruit. Background technique [0002] Russe fruit is the dry and mature fruit of Malus toringoides (Rehd.) Hughes. or Malus transitoria (Batal.) Schneid., a plant of the genus Rosaceae. It is used as traditional Tibetan medicinal materials in Ganzi Tibetan Autonomous Prefecture. With a long history, its flavonoids have the effects of lowering blood sugar, anti-oxidation, and protecting the liver. Patent CN109060985A discloses a method for detecting the quality of Russian fruit. The method achieves the purpose of quality detection by separating flavonoids in Russian fruit. However, it is found that Russian fruit also contains wolfberry acid (2-O-β -D-Glucopyranosyl-L-ascorbic Acid), this component was found in Rosaceae plants for the first time, and it was also found in Ascorbic Acid for the first time. [0003] According to literature reports, wolfberry acid is a natur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 罗枫
Owner SICHUAN ZHIJIACHENG BIO TECH CO LTD
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