Method for evaluating skin irritation of cosmetics
A technology for skin irritation and cosmetics, applied in the field of cosmetic safety evaluation, can solve problems such as low cost, achieve the effects of low cost, high spawning frequency, and convenient laboratory evaluation
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Embodiment 1
[0037] A method for evaluating skin irritation of cosmetics, wherein the mass content of glycerin in the cosmetics is 10%, and other components are mild and non-irritating, comprising the following steps:
[0038] (1) Determination of safe concentration: Place the transgenic neutrophil green fluorescent zebrafish 2 days after fertilization in a water-containing orifice plate with a water capacity of 3ml and 30 zebrafish, and add different concentrations of zebrafish to different orifice plates in a water-soluble manner. The concentration of the cosmetic to be tested is 1.39, 2.78, 5.56, 11.1, 22.2, 44.4 μL / mL. After 18 hours of culture, the death and organ damage of the zebrafish are counted. The results are shown in Table 1, so as to determine the safe concentration of the cosmetic The upper limit is 11.1 μL / mL;
[0039] (2) Set up a normal control group: 30 transgenic neutrophil green fluorescent zebrafish 2 days after fertilization were used as test subjects, placed in an o...
Embodiment 2
[0046] A method for evaluating the skin irritation of cosmetics, the content of methylisothiazolinone in the cosmetics is 70ppm, and the other components are all mild and non-irritating, comprising the following steps:
[0047] (1) Determination of safe concentration: Place the transgenic neutrophil green fluorescent zebrafish 2 days after fertilization in a water-containing orifice plate with a water capacity of 3ml and 30 zebrafish, and add different concentrations to different orifice plates in a water-soluble manner. The concentrations of the cosmetics to be tested were 1.39, 2.78, 5.56, 11.1, 22.2, and 44.4 μL / mL in turn. After 18 hours of culture, the death and organ damage of the zebrafish were counted. The results are shown in Table 1, so as to determine the safety of the cosmetics The upper limit of concentration is 5.56 μL / mL;
[0048] (2) Set up a normal control group: 30 transgenic neutrophil green fluorescent zebrafish 2 days after fertilization were used as test ...
Embodiment 3
[0055] A method for evaluating the skin irritation of cosmetics, the content of ethanol in the cosmetics is 3%, and the other components are mild and non-irritating, comprising the following steps:
[0056] (1) Determination of safe concentration: Place the transgenic neutrophil green fluorescent zebrafish 2 days after fertilization in a water-containing orifice plate with a water capacity of 3ml, 30 zebrafish, and add different concentrations to different orifice plates in a water-soluble manner. The concentrations of the cosmetics to be tested were 1.39, 2.78, 5.56, 11.1, 22.2, and 44.4 μL / mL in turn. After 18 hours of culture, the death and organ damage of the zebrafish were counted. The results are shown in Table 1, so as to determine the safety of the test. The upper limit of concentration is 22.2 μL / mL;
[0057] (2) Set up a normal control group: 30 transgenic neutrophil green fluorescent zebrafish 2 days after fertilization were used as test subjects, placed in an orifi...
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