Variable region sequence of broad-spectrum antibody resisting clothianidin and dinotefuran and preparation of recombinant complete antibody of variable region sequence

A variable region, dinotefuran technology, applied in the field of genetic engineering, to achieve the effect of facilitating modification and labeling, avoiding mutation and death

Active Publication Date: 2020-12-11
ZHEJIANG UNIV
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Problems solved by technology

Most of the recombinant intact antibodies reported so far are human antibodies, which are used in the medical field. Recombinant intact antibodies against small molecule pollutants such as pesticides are rarely reported

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  • Variable region sequence of broad-spectrum antibody resisting clothianidin and dinotefuran and preparation of recombinant complete antibody of variable region sequence
  • Variable region sequence of broad-spectrum antibody resisting clothianidin and dinotefuran and preparation of recombinant complete antibody of variable region sequence
  • Variable region sequence of broad-spectrum antibody resisting clothianidin and dinotefuran and preparation of recombinant complete antibody of variable region sequence

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Embodiment Construction

[0022] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative work should belong to the protection scope of the present invention. If there is no special description, the experimental methods in the embodiments are all for the conventional method.

[0023] The anti-clothianidin and dinotefuran recombinant intact antibody of the present invention comprises heavy chain constant region, heavy chain variable region, light chain constant region and light chain variable region, and its ...

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Abstract

The invention discloses a variable region sequence of a broad-spectrum antibody resisting clothianidin and dinotefuran. The amino acid sequence of a heavy chain variable region coding gene is shown asSEQ ID NO: 2. The invention also discloses a broad-spectrum recombinant complete antibody resisting clothianidin and dinotefuran. The broad-spectrum recombinant complete antibody resisting clothianidin and dinotefuran comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, and the amino acid sequence of the heavy chain variable region coding gene is shown as SEQ ID NO: 2. The sequence genes obtained by the invention are respectively connected to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, and a recombinant complete antibody is obtained by adopting mammalian cell expression of a double-plasmid system, so that the recognition activity of a murine parent antibody is ensured, the broad-spectrum antibody resisting clothianidin and dinotefuran can be stably produced on a large scale, and a reliable core reagent is provided for various immunoassay methods for synchronously detecting the clothianidin and the dinotefuran.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation and application of a broad-spectrum recombinant complete antibody against clothianidin and dinotefuran. Background technique [0002] Clothianidin is a second-generation neonicotinoid insecticide. Because it has a thiazole ring group, its performance is better than that of traditional neonicotinoid insecticides. As a high-efficiency broad-spectrum neonicotinoid Insecticides have been a large-scale insecticide worldwide in the past few years, and their usage and sales have remained at a high level every year. The toxicity of clothianidin to non-target organisms and the safety impact on the ecological environment research has attracted widespread attention. In the past two years, many countries around the world have introduced policies to restrict the use of clothianidin, and the European Union has now banned the use of clothianidin in outdo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/44C12N15/13C12N15/70
CPCC07K16/44C12N15/70C07K2317/56C07K2317/64C07K2317/92C07K2317/33C07K2317/20
Inventor 郭逸蓉常云云赵颖刘鹏琰柳颖陈阳朱国念
Owner ZHEJIANG UNIV
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