Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A culture-inducing medium for umbilical cord and placental mesenchymal stem cells

A technology of mesenchymal stem cells and induction fluid, applied in the direction of cell culture active agent, culture process, tissue culture, etc., can solve the problems of inability to promote skin fibroblasts, restore elastin secretion ability, and limited skin anti-aging effect, and achieve enhanced Elasticity, wrinkle reduction, and sagging effects

Active Publication Date: 2022-03-11
西安臻瑞生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have also shown that transplanting mesenchymal stem cells in aging skin can improve some aging indicators of cells, increase serum and skin SOD activity, but the overall skin anti-aging effect is limited
One of the essential reasons is that mesenchymal stem cells still cannot secrete elastin in the body after transplantation, and the factors secreted after mesenchymal stem cell transplantation cannot promote the restoration of elastin secretion ability of recipient skin fibroblasts, because adult cells almost lost the ability to secrete elastin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A culture-inducing medium for umbilical cord and placental mesenchymal stem cells
  • A culture-inducing medium for umbilical cord and placental mesenchymal stem cells
  • A culture-inducing medium for umbilical cord and placental mesenchymal stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] Based on the fact that the mesenchymal stem cells in the umbilical cord and placenta have the ability to differentiate into skin fibroblast-like cells, while the umbilical cord and placenta derived from newborns still have the ability to secrete elastin, if the mesenchymal stem cells in the umbilical cord or placenta are in vitro Induce skin fibroblast-like cells in a special way, and stimulate these induced cells to secrete elastin, and then transplant these cells into aging skin, so that these transplanted cells can continue to secrete elastin, which can achieve a good skin Anti-aging effect. Therefore, the present invention has developed a specific culture induction solution for umbilical cord and placental mesenchymal stem cells, which can produce elastin-secreting stem cells through in vitro culture for skin anti-aging.

[0020] The culture induction solution of umbilical cord and placental mesenchymal stem cells uses α-MEM liquid medium as a solvent to dissolve th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a culture induction solution for umbilical cord and placental mesenchymal stem cells, using α-MEM liquid medium as a solvent to dissolve the following components: calcium chloride 2-5mmol / L, potassium chloride 1-5mmol / L L, TGF‑β5~20ng / mL, bFGF 5~20ng / mL, EGF 5~20ng / mL, IGF‑1 5~20ng / mL, transferrin 10~30ng / mL, hesperidin 20~100ng / mL , selenous acid 5-50ng / mL, linoleic acid 5-50ng / mL, bovine serum albumin 0.5-5ng / mL. The culture medium induces cultured umbilical cord or placental mesenchymal stem cells for skin transplantation, which can enhance the elasticity of the skin and improve the aging state of the skin.

Description

technical field [0001] The invention relates to a culture induction solution for umbilical cord and placenta mesenchymal stem cells. Background technique [0002] Now the skin is the largest organ in the human body, which has the functions of protection, excretion, temperature regulation and feeling of external stimuli, and is also the external manifestation of human aging. With the increase of age, a series of degenerative lesions will occur in the human skin, the skin becomes thinner, the elasticity fades, and wrinkles appear. [0003] There are many mechanism theories of skin aging, such as gene disorder theory, endocrine disorder theory, free radical theory, glycosylation, and immune decline theory. But no matter which theory, skin aging must be accompanied by the decline of elastic fiber content in the skin. [0004] Elastic fibers are mainly in the dermis of the skin, forming an elastic fiber network to maintain the elasticity of the skin. Mature elastic fibers are ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/071
CPCC12N5/0668C12N5/0656C12N2500/14C12N2500/12C12N2500/24C12N2501/15C12N2501/115C12N2501/11C12N2501/105C12N2500/05C12N2500/36C12N2501/998
Inventor 罗海浪徐佳洁张敏
Owner 西安臻瑞生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com