Method for detecting metabolites and kit thereof

A technology for metabolites and analytes, applied in the field of metabolic detection, can solve problems such as quantitative detection of difficult components, and achieve the effects of reduced impact, high reaction selectivity and high precision

Inactive Publication Date: 2020-12-18
必睿思(杭州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, due to the fact that the metabolites produced by the existing human metabolism are trace amounts, it is difficult for existing detection methods to accurately and quantitatively detect the components in exhaled breath or body fluids

Method used

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  • Method for detecting metabolites and kit thereof
  • Method for detecting metabolites and kit thereof
  • Method for detecting metabolites and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] (1) Preparation of selective adsorption device

[0035] That is, for the preparation of adsorption sampling tubes, organic solvents such as n-hexane, ethyl acetate and acetonitrile are used to prepare a coating solution containing derivatized reagents with a content of 0.01%-1%; the volume of 36%-38% concentrated hydrochloric acid in the coating solution The content is 0.1%. Then fill 200 mg of stationary phase filler (silica gel, C18, tenax-GR, etc.) onto the blank adsorption column; finally, coat the coating liquid on the adsorption column filled with stationary phase, and the coating method adopts the direct coating method. .

[0036] Derivatization reagents can be 2,4-dinitrophenylhydrazine (DNPH), pentafluorophenylhydrazine (PFPH), O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA) .

[0037] Before coating, rinse the blank adsorption column with the corresponding solvent such as n-hexane, ethyl acetate, acetonitrile, etc., and remove the rinsed ...

Embodiment 1

[0045] The configuration of the enhancer: according to the ratio of glucose and water 1:4-50, it is configured into a glucose aqueous solution. The subjects ingested an aqueous glucose solution orally, and the total glucose intake was at least 0.1 g. After ingestion, the air exhaled by the human body was collected by the pretreated air bag, and the air bag was collected at 10 minutes, 25 minutes, and 55 minutes, respectively. (The time points for the air bag to collect the exhaled breath of the human body include but are not limited to 10 minutes, 25 minutes, and 55 minutes.)

[0046] Preparation of adsorption sampling tubes

[0047] 2,4-Dinitrophenylhydrazine (DNPH) was prepared with n-hexane into a coating solution containing 0.1% of DNPH; the adsorption sampling tube was prepared according to the steps in (1), and n-hexane was used to rinse before coating Blank adsorption column.

[0048] Human exhaled gas collection and processing

[0049] After drinking the aqueous gl...

Embodiment 2

[0069] The configuration of strengthening agent: according to the ratio of starch and 100 ℃ water 1:2-100, configure into edible starch solution. The total starch intake was 75 g, which the subjects ingested orally. Before ingestion, the pretreated air bag was used to collect the exhaled air of the human body, and after ingestion, the air bag was collected at 0, 5, 30, 60, 90, and 120 minutes respectively. (The time point when the air bag collects the exhaled breath of the human body includes but is not limited to the above time.)

[0070] Preparation of adsorption sampling tubes

[0071] 2,4-Dinitrophenylhydrazine (DNPH) was prepared with acetonitrile into a coating solution containing derivatized reagent with a content of 0.1%; the adsorption sampling tube was prepared according to the steps in (1), and acetonitrile was used to rinse the blank before coating. adsorption column.

[0072] Human exhaled gas collection and processing

[0073] The exhaled breath of the human ...

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Abstract

The invention provides a method for detecting metabolites and a kit thereof, and belongs to the technical field of metabolic detection. The method solves the problems that a trace amount of metabolites is generated by existing human metabolism, and components in exhaled air or body fluid are difficult to accurately and quantitatively detect through an existing detection method; and the method comprises the following steps: S01, enabling a reinforcing agent to be supplied to a subject for intake, and then collecting the body fluid or exhaled air of the subject; S02, converting the exhaled gas or body fluid into an object to be detected; and S03, detecting the content of a target detection object in the body fluid or exhaled breath. The method has high detection sensitivity.

Description

technical field [0001] The invention belongs to the technical field of metabolic detection, and particularly relates to a method for detecting metabolites and a kit thereof. Background technique [0002] The exhaled breath and body fluids of the subjects are closely related to the survival and metabolism of the human body and the survival and metabolism of bacteria. There are many kinds of bacteria in the body, and the composition of the flora can greatly affect the health of the human body. The gas and body fluids exhaled by the human body are used to analyze the components of the gas or body fluids with specific detection methods, and then the difference in values ​​can be distinguished, which can indirectly reflect the metabolic status of the human body and provide medical personnel with the direction of follow-up diseases. [0003] However, since the existing metabolites produced by human metabolism are in trace amounts, it is difficult for the existing detection methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72A61B5/083
CPCG01N30/02G01N30/06G01N30/7206A61B5/083G01N2030/067
Inventor 赵伟军周伟史夏青章旭日汪小知张厚德郝雷段兴斌
Owner 必睿思(杭州)科技有限公司
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