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Long-arm activated biotin molecular marker antigen as well as preparation method and application thereof

A technology of activated biotin and molecular labeling, which is applied in the field of long-arm activated biotin molecular labeling antigen and its preparation, which can solve the problems of missed detection or false detection, low positive rate, and insufficient sensitivity

Active Publication Date: 2020-12-22
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing biotin-labeled antigens have the disadvantages of insufficient sensitivity and low positive rate in the detection of antibodies, and there are risks such as missed or false detections, and none of them can meet the requirements.

Method used

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  • Long-arm activated biotin molecular marker antigen as well as preparation method and application thereof
  • Long-arm activated biotin molecular marker antigen as well as preparation method and application thereof
  • Long-arm activated biotin molecular marker antigen as well as preparation method and application thereof

Examples

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preparation example Construction

[0039] Preparation of biotin-labeled antigen (arm length 29)

[0040] Take 0.5 mg of HBsAg recombinant antigen, dialyze with 0.01M pH=8.5 borate solution at 4°C for 2 hours, add 25 μg of long-arm activated biotin to the dialyzed antigen, and add dimethyl sulfoxide at the same time to make dimethyl The final mass concentration of sulfoxide is 5%, shake slowly, and react in the dark for 4 hours, add 250 μL of 5M ammonium chloride solution to the above solution, and react in the dark at room temperature for 30 minutes; dialyze with 0.02M PBS solution at 4°C for 24 hours, and change the medium during the period 3 times.

[0041] Preparation of streptavidin-coupled magnetic particle suspension

[0042] Take 100ml of 0.1M 2-morpholineethanesulfonic acid (MES) buffer solution, add 10mg of magnetic particles with carboxyl and amino groups coupled on the surface, stir at room temperature for 40min, then add 3.5mg streptavidin, and then add 8mg / ml carbon Diimine solution (EDC) was rea...

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Abstract

The invention provides a long-arm activated biotin molecular marker antigen as well as a preparation method and application thereof. Long-arm activated biotin is a long-arm activated biotin marker antigen, so that the detection sensitivity and specificity can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, and in particular relates to a long-arm activated biotin molecule labeled antigen and a preparation method and application thereof. Background technique [0002] In recent years, the use of the affinity between biotin and avidin, as well as the coupling of macromolecular biologically active substances such as antigens and antibodies between them, and the ability to be absorbed by various materials such as enzymes Marked characteristics, developed a new type of biological reaction amplification system, that is, biotin-avidin system (Biotin-Avidin System), and established a variety of high sensitivity, high specificity, high stability, convenient, economical and rapid Biological reaction monitoring and separation and purification. [0003] However, the existing biotin-labeled antigens have the disadvantages of insufficient sensitivity and low positive rate in antibody detection, and there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/02C07K1/13G01N33/569G01N33/543G01N33/535
CPCC07K14/005G01N33/56983G01N33/535G01N33/54326C12N2730/10122
Inventor 栾大伟刘萍张振斌田鸽史光辉李华
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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