Variable region sequence of specific anti-thiacloprid antibody and anti-thiacloprid recombinant overall-length antibody

Abstract

The invention discloses a variable region sequence of a specific anti-thiacloprid antibody. The amino acid sequence of a heavy chain variable region encoding gene is disclosed by SEQ ID NO: 2. The invention also discloses an anti-thiacloprid recombinant overall-length antibody, which comprises a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein the amino acid sequence of the heavy chain variable region encoding gene is disclosed by SEQ ID NO: 2. The invention also discloses an antibody expression plasmid. A sequence gene obtained by the invention is independently connected to expression carriers containing a heavy chain constant region gene and a light chain constant region gene, mammal cell of a double-plasmid system is adopted to obtain the recombinant overall-length antibody, the identification activity of a murine parent antibody is guaranteed, the anti-thiacloprid antibody can be stably produced ona large scale, and a reliable core reagent is provided for various immunoassay methods for detecting thiacloprid residues.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the preparation of a variable region sequence of a specific anti-thiamethoxam antibody and a recombinant full-length antibody thereof. Background technique [0002] Neonicotinoid insecticides have become the fastest growing insecticides in the global market and are widely used in more than 120 countries around the world. This type of insecticide inhibits the acetylcholinesterase receptor of the central nervous system of the pest, blocks the normal conduction of the central nervous system of the pest, and causes the paralysis of the pest to death. etc. have a good control effect. Thiamethoxam (3-(2-chloro-1,3-thiazol-5-ylmethyl)-5-methyl-1,3,5-oxadiazin-4-ylidene(nitro)amine) is A second-generation neonicotinoid insecticide with a new structure has strong systemic and osmotic effects, so it will be distributed in various parts of the crop, and because of ...

AI Technical Summary

Problems solved by technology

Among the many recombinant antibody expression systems, the mammalian cell expression system can guide the correct folding of recombinant antibodies and provide accurate various post-translational processing and modification functions, so it can stably produce recombinant antibodies with the same activity as the parental antibody, which not only confirms The ac

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