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Method for establishing hermetia illucens embryo microinjection platform

A microinjection and embryo technology, applied in the biological field, can solve the problems of microinjection and inability to carry out embryos, and achieve the effects of increasing the survival rate, simple methods of egg collection and egg placement, and maintaining healthy and rapid development.

Pending Publication Date: 2020-12-22
江苏绿博低碳科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limitation that microinjection of embryos cannot be performed, many laboratories cannot carry out genetic experiments. Our laboratory has a very good platform for studying the Lepido

Method used

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  • Method for establishing hermetia illucens embryo microinjection platform
  • Method for establishing hermetia illucens embryo microinjection platform
  • Method for establishing hermetia illucens embryo microinjection platform

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1. Put 50 mated females in a strong light-irradiated cage with 5-10 pieces of corrugated paper as ovipositors to collect eggs, observe every 20 minutes, and collect eggs immediately after they are laid. figure 1 The egg collection device showing the eggs of the black soldier fly.

[0029] 2. The collected egg masses are immediately peeled off gently with a fine brush, and separated on a glass slide. They are arranged in the order of the egg hole to the left, with 30 eggs in each row, and 6-8 rows in each plate. figure 2 Shows the arrangement of black soldier fly eggs.

[0030] 3. Place the ovulated eggs on the microinjection table. The temperature of the capillary glass tube used for injection is controlled at 60°C when the needle is pulled, and the angle between the capillary glass needle and the egg is controlled at 45° during the injection angle. For the lower part of the embryo, the entire injection time (from egg collection to completion of injection) was control...

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Abstract

The invention discloses a hermetia illucens embryo microinjection method. The method comprises the following steps: (1) collecting eggs newly laid by mated hermetia illucens female individuals; (2) neatly placing the eggs obtained in the step 1) on a glass slide; (3) placing the glass slide on a microinjection table for microinjection, controlling the temperature of a capillary glass tube which isused to be 60-65 DEG C during needle pulling, controlling an injection angle to be an included angle between a capillary glass needle and an egg to be 20-45 degrees, controlling the injection position to be at the lower part of the embryo, and controlling the time from egg collection to injection completion to be within 0.5-2 hours; and (4) performing putting into an incubator at 24-26 DEG C, controlling the humidity to be 50-80%, and continuously performing culturing in the dark. A hermetia illucens egg collection technology and a micromanipulation technology are combined, so that the hermetia illucens embryo microinjection platform is successfully established. An egg collecting and placing method is simple, time-saving and labor-saving, and the survival rate of embryos can be greatly increased by strict implementing according to injection conditions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for microinjection of black soldier fly embryos. Background technique [0002] Hermetia illucens L. belongs to Diptera, Soldieridae, commonly known as black soldier flies, and is an important resource insect. The larvae feed on naturally decomposed organic matter and animal waste. If they make good use of this feature, they will show great application value in environmental governance. The life cycle of black soldier flies is 40-45 days. It can be artificially raised on a large scale, and can efficiently handle food waste and livestock and poultry manure. In the process of feeding on livestock and poultry manure, the black soldier fly larvae can significantly reduce various types of feces. odor. [0003] The black soldier fly larvae can accumulate a large amount of protein and fat in the process of converting organic wastes. The protein content of the dry worm...

Claims

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Application Information

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IPC IPC(8): C12N15/89
CPCC12N15/89
Inventor 黄勇平王四宝詹帅许军寇宗庆
Owner 江苏绿博低碳科技股份有限公司
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