Rapid selective breeding method of high-yield and disease-resistant mango saplings

Abstract

The invention provides a rapid selective breeding method of high-yield and disease-resistant mango saplings. The rapid selective breeding method comprises the following steps that (1) a mango variation single plant is selected; (2) stems with axillary buds are taken, sterilization treatment is carried out, dark pre-culture is carried out on the stems in a 1 / 2MS culture medium containing polyvinylpyrrolidone and silver nitrate, the stems are transferred into a 1 / 2MS culture medium containing polyvinylpyrrolidone, TDZ and Na2S2O3 for dark transition culture, and the stems are transferred into asubculture medium for culture; (3) seedlings are transferred into a seedling hardening bed for seedling hardening culture, and the seedlings are transferred into a nutrition cup containing a seedlinghardening strengthening matrix for strengthening seedling hardening treatment when the seedlings grow to be 10-15cm high; and (4) culturing is carried out at room temperature until the plant height ismore than or equal to 35cm, plants are planted to a field, and a single plant with stable hereditary excellent characters is selected as a new variety; By means of the rapid selective breeding method, the phenol pollution influence in the mango seedling tissue culture process is effectively eliminated, the transplanting survival rate and growth quality of the mango seedlings are remarkably improved, and mango sapling selective breeding and application and popularization are accelerated.

Description

technical field [0001] The invention relates to the technical field of mango breeding, in particular to a rapid breeding method for high-yield and disease-resistant mango saplings. Background technique [0002] Mango is one of the famous fruits in the tropics and subtropics. Mango cultivation areas are mainly distributed in Hainan, Guangxi, Guangdong, Yunnan and the dry-hot valley of the Jinsha River in Sichuan, which has high economic and social value. In the field of selective breeding of mangoes, different degrees of research have been carried out from seed selection breeding, artificial cross breeding to mutation breeding, and some effective results have been achieved. However, because mangoes are highly heterozygous woody plants, the The survival period is short, the traditional breeding method is difficult to improve mango varieties, and the cycle is long; however, the research on the method of mango seedling selection by tissue culture is quite weak, which often has s...

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Problems solved by technology

In the field of selective breeding of mangoes, different degrees of research have been carried out from seed selection breeding, artificial cross breeding to mutation breeding, and some effective results have been achieved. However, because mangoes are highly heterozygous woody plants, the The survival period is short, the traditional breeding method is difficult to improve mango varieties, and the cycle is long; however, the research on the method of mango seedling selection by tissue culture is quite weak, which often has serious phenol damage during the tissue culture process and the survival of the tissue culture seedlings after transplanting. Therefore, to find a rapid breeding method for high-yield, disease-resistant mango seedlings, to establish a reliable and efficient high-yield, rapid breeding process for disease-resistant mango seedling plants, for the rapid selection and breeding of excellent mango varieties It is important to promote the use of

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