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Application of CLEC2B gene in differential diagnosis of tuberculosis

A technology of tuberculosis and markers, applied in the application field of molecular marker CLEC2B in the diagnosis of tuberculosis, can solve problems such as inability to distinguish between tuberculosis and non-tuberculous mycobacterium infection, confusion of lung diseases, and inability to determine whether it is active tuberculosis or not , to achieve the effect of improving the detection rate of clinical tuberculosis

Inactive Publication Date: 2020-12-29
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is easy to be confused with other lung diseases and needs to be confirmed by a professional doctor
(3) The host’s immune response detection methods, such as tuberculin skin test (tuberculin skin test, TST) and interferon gamma release assays (interferon gamma release assays, IGRA), can only determine whether tuberculosis infection is active or not Tuberculosis and inability to distinguish tuberculosis from nontuberculous mycobacterial infection
) was recently reported to be related to tumor progression, platelet aggregation, etc., but no research reports related to tuberculosis have been found so far

Method used

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  • Application of CLEC2B gene in differential diagnosis of tuberculosis
  • Application of CLEC2B gene in differential diagnosis of tuberculosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1, detection method

[0084] 1.1 Research objects and methods

[0085] 1.1.1 Research object

[0086] Research Objects and Inclusion Criteria

[0087] The subjects of this study included active tuberculosis, latent tuberculosis infection and normal control group. Active tuberculosis (Active Tuberculosis, ATB) came from the confirmed inpatients of Shenzhen Third People's Hospital. The diagnostic criteria were based on the "Health Industry Standards of the People's Republic of China (WS 288-2017) Diagnosis of Tuberculosis", and the etiology test was positive: sputum smear At least one of the three items of / culture / nucleic acid test is positive, there is no previous history of tuberculosis (no old tuberculosis lesions in the consultation and X-ray chest examination), the first anti-tuberculosis treatment, the drug is less than 7 days; latent infection (LTBI, Latent Tuberculosis Infection) had a clinical exposure history, no clinical symptoms, and IGRA-positiv...

Embodiment 2

[0127] Embodiment 2, test kit

[0128] The kit of the present invention consists of the following components:

[0129] 1. Specific cell enrichment antibody-labeled magnetic beads, Dynabeads CD15 (Invitrogen, US)

[0130] 2. Cell total RNA extraction kit, RNeasy Plus Mini Kit (Qiagen, Germany)

[0131] 3. cDNA reverse transcriptase, SuperScript TM IV VILO TM Master Mix (Invitrogen, US)

[0132] 4. qPCR fluorescent quantitative PCR detection system, including PCR polymerase, buffer system, and Assay CLEC2B exon region-specific detection primers and probes.

[0133] The present invention reports for the first time that the detection of CLEC2B gene expression changes in specific cells contained in human peripheral blood is more sensitive and specific than the detection of related gene expression changes in peripheral blood and whole blood. Through the combination of a series of commercial reagents and process optimization, it is possible and practical to use the gene as a...

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Abstract

The invention belongs to the field of biological medicines, and relates to an application of a molecular marker CLEC2B in tuberculosis diagnosis. The invention provides an application of a reagent fordetecting the expression level of the molecular marker CLEC2B in preparation of a kit for diagnosing tuberculosis.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of a molecular marker CLEC2B in the diagnosis of tuberculosis. Background technique [0002] Tuberculosis (TB) is a chronic infectious disease that seriously threatens human health caused by Mycobacterium tuberculosis (M.tb) infection. Globally, there are more than 10 million new cases of tuberculosis and 1.3 million deaths each year. At the same time, nearly a quarter of people are infected with Mycobacterium tuberculosis and remain in a state of latent infection for a long time, and 5% to 10% of them may develop active tuberculosis in their lifetime. Active tuberculosis patients are only the tip of the iceberg of tuberculosis-infected patients. The progression from latent Mycobacterium tuberculosis infection to active disease poses a persistent threat to the spread of tuberculosis. One of the important components of control. Due to the biological characteristics of My...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883G01N33/68C12N15/10
CPCC12Q1/6883G01N33/6893C12N15/1013C12Q2600/158G01N2800/26
Inventor 金奇张笑冰刘立国
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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