Corneal endothelial membrane based on human corneal stroma as carrier
A technology of corneal endothelium and corneal stroma, applied in the field of microbial detection, can solve the problems of lack of corneal donors and patients' lack of treatment, and achieve the effect of good scaffold materials
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] 1) Take the donated human cornea as the donor material, set the laser / keratome parameters, set the cutting thickness to 50 μm, and set the diameter to 8 mm, then put the donor material in the machine, and cut out a thickness of 50 μm each time Thin slices (hereinafter referred to as matrix flakes) ( figure 1 ), the obtained matrix flakes were collected, placed in a sterile EP tube, sealed and placed in a negative 80°C refrigerator for storage;
[0026] 2) In the ultra-clean bench, take out the cryopreserved matrix slices from the EP tube and transfer them to a sterile centrifuge tube; wash the matrix slices with PBS for 3 times, 5 min each time;
[0027] 3) Transfer the matrix slice to a new sterile centrifuge tube, slowly add double antibody (penicillin-streptomycin mixture) for cell culture into the tube until the matrix slice is completely submerged, and shake the centrifuge tube for 1 min;
[0028] 4) Transfer the matrix slice to a new sterile centrifuge tube, was...
PUM
Property | Measurement | Unit |
---|---|---|
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
thickness | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com