Corneal endothelial membrane based on human corneal stroma as carrier

A technology of corneal endothelium and corneal stroma, applied in the field of microbial detection, can solve the problems of lack of corneal donors and patients' lack of treatment, and achieve the effect of good scaffold materials

Pending Publication Date: 2021-01-01
PEKING UNIV THIRD HOSPITAL
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  • Claims
  • Application Information

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Problems solved by technology

[0003] A large proportion of patients suffer from corneal endothelial blindness caused by corneal endothelial decompensation. At present, the most effective way to treat this disease is endothelial corneal transplantation. However, due to the shortage of corneal donors, many patients cannot receive corresponding treatment. Being able to use tissue engineering to construct corneal endothelial grafts would greatly alleviate this problem

Method used

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  • Corneal endothelial membrane based on human corneal stroma as carrier
  • Corneal endothelial membrane based on human corneal stroma as carrier
  • Corneal endothelial membrane based on human corneal stroma as carrier

Examples

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Effect test

Embodiment 1

[0025] 1) Take the donated human cornea as the donor material, set the laser / keratome parameters, set the cutting thickness to 50 μm, and set the diameter to 8 mm, then put the donor material in the machine, and cut out a thickness of 50 μm each time Thin slices (hereinafter referred to as matrix flakes) ( figure 1 ), the obtained matrix flakes were collected, placed in a sterile EP tube, sealed and placed in a negative 80°C refrigerator for storage;

[0026] 2) In the ultra-clean bench, take out the cryopreserved matrix slices from the EP tube and transfer them to a sterile centrifuge tube; wash the matrix slices with PBS for 3 times, 5 min each time;

[0027] 3) Transfer the matrix slice to a new sterile centrifuge tube, slowly add double antibody (penicillin-streptomycin mixture) for cell culture into the tube until the matrix slice is completely submerged, and shake the centrifuge tube for 1 min;

[0028] 4) Transfer the matrix slice to a new sterile centrifuge tube, was...

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Abstract

The invention relates to a corneal endothelial membrane based on a human corneal stroma as a carrier. The average thickness of the human corneal stroma as the carrier is 20-100 [mu]m, preferably 20-70[mu]m; the carrier is provided with a modification layer for blocking water and promoting cell adhesion, and the modification layer is inoculated with corneal endothelial cells. By adopting the humancorneal stroma with smaller average thickness, one donor material can be cut into a plurality of slices, so that the maximum value of the donor material is exerted, and a good stent material can be provided for the construction of the endothelial membrane.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a corneal endothelial membrane based on human corneal stroma as a carrier and a manufacturing method thereof. Background technique [0002] Corneal disease is the second leading cause of blindness in the world after cataract. Of the 45 million blind people in the world, 8 million are blinded by corneal diseases, and an average of 1.5-2 million new cases of blindness due to corneal ulcers and trauma are added each year. Corneal transplantation is currently the main treatment for severe corneal diseases. Although there are more than 1 million blind patients due to corneal diseases in my country, there are less than 5,000 cases of corneal transplantation each year. The lack of corneal donors has always been and is increasingly becoming an important constraint for corneal transplantation. factor. [0003] A large proportion of patients suffer from corneal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/38A61L27/50A61L27/24A61L27/22
CPCA61L27/3604A61L27/3641A61L27/3808A61L27/3839A61L27/50A61L27/24A61L27/227A61L2430/16C08L89/00
Inventor 洪晶彭荣梅卢青
Owner PEKING UNIV THIRD HOSPITAL
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