Pseudomonas and application thereof

A pseudomonas and strain technology, applied in the field of microorganisms, can solve the problems affecting the growth and development of fruit trees, apple yield and quality, apple leaf shedding, environmental pollution, etc., and achieves good isolated leaf control effect, convenient application, and simple preparation process. Effect

Active Publication Date: 2021-01-05
烟台市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevalence of the disease can cause a large number of apple leaves to fall off early, seriously affecting the growth and development of fruit trees and the yield and quality of apples
[0004] At present, the prevention and control of apple leaf spot and apple brown spot mainly rely on chemical pesticides. Although chemical control is low in cost and quick in effect, long-term use will bring problems such as pathogen resistance, fruit pesticide residues, human health, and environmental pollution.

Method used

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  • Pseudomonas and application thereof
  • Pseudomonas and application thereof
  • Pseudomonas and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Isolation and identification of Pseudomonas sp. of the present invention

[0025] A. Isolation and preservation of Pseudomonas sp.

[0026] Step 1) Specimen collection: Collect the rhizosphere soil of healthy apple trees in Penglai District, Yantai City, Shandong Province, China, and put them in sterile bags for later use.

[0027] Step 2) Separation and purification of Pseudomonas sp.: Air-dry the collected soil samples and grind them. Weigh 10 g of the soil sample and put it into an Erlenmeyer flask, add 90 mL of sterile water, shake on a shaker for 30 minutes, and obtain a suspension of the soil sample. Draw 1mL of the suspension, add it into a small test tube filled with 9mL of sterile water, mix well, and obtain a concentration of 10 -2 The soil sample dilution solution, using the same method to obtain a concentration of 10 -5 、10 -6 soil sample dilution. Draw 100 μL of each of the two concentrations of dilutions and spread them on the NA medium with a coating...

Embodiment 2

[0039] Antibacterial activity analysis of Pseudomonas sp. of the present invention

[0040] The antibacterial activity analysis of the described Pseudomonas sp. was carried out by the plate confrontation growth method: with the apple leaf leaf spot and apple brown leaf spot as the indicator bacteria, the activated indicator bacteria cake was punched with a 6mm punch and placed In the center of the plate, inoculate Pseudomonas sp by spot-joining method, the distance between the two bacteria is about 4.5cm, and on the diameter of one line, the control group is only inoculated with the indicator bacteria cake, repeated 3 times, and cultured in the dark at 28°C. After the control group covered the plate, observe the growth of pathogenic bacteria in the test group, and measure the antibacterial zone ( image 3 , Figure 4 ,Table 2).

[0041] Table 2. Antibacterial activity analysis of Pseudomonas sp. against two indicator bacteria

[0042] Indicator bacteria Control ...

Embodiment 3

[0044] Antibacterial activity analysis of fermentation filtrate of Pseudomonas sp. of the present invention

[0045] After activation, the Pseudomonas sp. of the present invention was inserted into a 250mL conical flask containing 100mL fermentation medium at a volume ratio of 2%, and the fermentation broth was obtained by shaking at 30°C and 200rpm for 72h, and the fermentation broth was subjected to 5000r / min After centrifugation for 10 min, the supernatant was filtered and sterilized with a 0.22 μm microporous filter to obtain a fermentation filtrate. The fermentation filtrate was mixed with the sterilized PCDA medium at about 50°C in a volume ratio of 1:50, and then poured into the plate. Using the apple leaf leaf spot and apple brown leaf spot as the indicator bacteria, use a 6mm punch to punch out the activated ones. The indicator bacteria cake was placed in the center of the plate, and the growth rate method was used to analyze the antibacterial activity of the fermenta...

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Abstract

The invention discloses pseudomonas sp. CGMCC No. 20361. The pseudomonas sp. CGMCC No. 20361 is finally identified as pseudomonas sp through morphological characteristic observation, physiological andbiochemical reaction and 16S rDNA sequence comparison of a strain; and the strain and the fermentation filtrate thereof have antagonistic activity on alternaria mali and alternaria mali. The strain is simple in culture condition, simple in fermentation filtrate preparation process and convenient to apply, and has the potential of developing a novel and efficient biocontrol microbial agent for apple related diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Pseudomonas sp, and also relates to the application of the bacterial strain and its fermented liquid. Background technique [0002] Apples are rich in various elements such as carbohydrates, vitamins and minerals, and have extremely high nutritional value. my country has a long history of planting apples, which can be traced back to the Wei and Jin Dynasties. Since the foreign variety "Big Apple" was introduced into my country in the 1870s, it has gradually developed into the main variety of apple cultivation in my country because of its better quality (Zhang Yuxing 2003). At present, my country has become the largest apple production and consumption country in the world. With the improvement of intensive production and excessive simplification of cultivated varieties, the occurrence of apple diseases and insect pests is becoming more and more common and ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P3/00C12R1/38
CPCA01N63/27C12R2001/38C12N1/205
Inventor 路兆军苗杰刘瑞珍王淑惠马旭升王益星赵莹
Owner 烟台市林业科学研究所
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