Disclosed in the present invention is a fungicidal composition containing prothioconazole and a prochloraz manganese salt and use thereof. The fungicidal composition uses prothioconazole and a prochloraz manganese salt as the main active ingredients. The fungicidal composition may be applied to control cereal, fruit, and vegetable diseases, and has high synergistic effects, overcomes and delays the resistance of pathogenic fungi, and has a fast fungicidal speed and long duration. The application cost is reduced, and the control effect is significantly better than that of a single agent. The present invention may be used to control fungal diseases on crops, and in particular, can be used to control powdery mildew, sheath blight, fusarium wilt, leaf spot, rust disease, stalk break, net blotch, scald disease, gray mold, black spot, brown blotch, black shank, cucumber, citrus and pimento anthracnose, citrus stem-end rot, green mold, penicilliosis and mushroom brown rot, and the effects are better than that of a single agent.