32 results about "Brown blotch disease" patented technology

The invention provides an organic liquid suspension fertilizer as well as a preparation method and application thereof. Comprising the following components in parts by weight: 10-15 parts of chitosan mother liquor, 12-16 parts of amino acid fermentation mother liquor, 23-27 parts of gibberellin mushroom dreg fermentation liquor, 30-35 parts of vinasse fermentation liquor, 5-10 parts of potassium humate, 1-3 parts of a surfactant, 12-15 parts of a nitrogen fertilizer, 10-14 parts of a phosphate fertilizer, 26-31 parts of a potassium fertilizer, 3-8 parts of a trace element mixture and 35-40 parts of small molecule water. The organic liquid suspension fertilizer prepared by the invention can be used as a crop fertilizer, especially can improve the quality of potatoes and the yield and sugar content of beet, and reduces brown spot.

The invention relates to the technical field of microbial control, and discloses a bacillus velezensis nano-material complex microbial inoculant as well as a preparation method and application thereof. The microbial inoculum is prepared by the following steps: preparing a bacterial suspension of bacillus velezensis; mixing sodium alginate with the bacterial suspension, adding nanocarbon, and stirring to obtain a mixed solution; and adding the mixed solution into a stirred calcium chloride solution, and reacting to obtain the bacillus velezensis microcapsule fungicide. The bacteriostasis rates of the fungicide for citrus brown spot, citrus colletotrichum gloeosporioides, citrus lipolytic macular blight, banana fusarium wilt and banana long leaf spot bacteria are all greater than 56%; the prevention and treatment effect on the Huanglongbing reaches 57.84%. After ultraviolet irradiation, the bacteriostatic ability of the microbial agent is reduced by only 4.32%, and the microbial agent can play a biological prevention and control role for a long time. The growth amount of citrus seedlings treated by the microbial inoculum can be increased by 220.39%, the number of newly grown leaves can be increased by 244.67%, and the microbial inoculum has a good plant growth promoting effect.

The invention discloses marine-derived penicillium as well as a fermentation method and application thereof. The marine source penicillium is named as Penicillium sp.DSF059, and the preservation number of the marine source penicillium is CCTCC (China Center For Type Culture Collection) NO: M 20232310. According to the invention, the culture conditions of the strain are optimized, the culture medium and culture conditions with high yield of antibacterial active compounds are selected for fermentation, more target products can be obtained, the operation is simple, the cost is low, and the method is suitable for industrial production. Meanwhile, the marine-derived penicillium sp. DSF059 can provide an excellent strain for the development of new drugs for resisting plant pathogens such as coffee leaf blight, coffee brown spot, grape gray mold, golden cypress brown spot, citrus black rot, phytophthora capsici, hickory leaf blight and coffee black spot as well as four pathogens separated from coffee leaf diseases and staphylococcus aureus; good application prospects are realized on prevention and treatment of phytopathogen and staphylococcus aureus.

The invention relates to the technical field of microbial control, and discloses bacillus velezensis E17-23 and application thereof. The bacillus velezensis E17-23 disclosed by the invention can be used for inhibiting citrus liberobacter asiaticum, citrus canker pathogen, citrus brown spot pathogen, citrus colletotrichum gloeosporioides, citrus lipolytic yellow spot pathogen, banana fusarium wilt pathogen and banana long leaf spot pathogen, and the inhibition effects are all greater than 60%. The inhibition effect of the sterile fermentation filtrate of the bacillus velezensis E17-23 on the pathogenic bacteria is more than 20.83%. The bacillus velezensis E17-23 can also promote plant growth, the average growth amount of treated orange seedlings is increased by 177.57% compared with that of a control group, and the number of new leaves is increased by 208.5% compared with that of the control group. The application of the bacillus velezensis E17-23 in the aspects of biological control and plant growth promotion has important significance in green agriculture and sustainable agricultural production.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The application discloses a marine source penicillium and a fermentation method and application thereof. The marine source penicillium is named Penicillium sp. DSF059, and the preservation number is CCTCC NO: M 20232310. Penicilliu The application optimizes the culture condition of the strain, selects a culture medium and culture condition with high yield of antibacterial active compounds to carry out fermentation, so that more target products can be obtained, the operation is simple, the cost is low, and meanwhile, the marine source penicillium Penicilliu Penicillium sp. DSF059 can provide an excellent strain for new drug development of resisting plant pathogenic fungi such as coffee leaf blight, coffee brown spot disease, grape gray mold disease, Chinese cinnamon brown spot disease, citrus black rot disease, pepper late blight, hickory leaf blight, coffee black spot disease and four pathogenic fungi separated from coffee leaf diseases and Staphylococcus aureus, and has a good application prospect for preventing and treating the plant pathogenic fungi and Staphylococcus aureus.

The invention provides a pseudomonas paraxanthomonas strain 1653 and a biological preparation and application thereof, and relates to the technical field of biological control, the pseudomonas paraxanthomonas strain 1653 is collected from citrus leaves, isolated and cultured through a tissue isolation method to obtain a strain, and the strain is obtained through directional screening. The biological agent of the pseudomonas paraxanthomonas strain 1653 has a remarkable inhibition effect on citrus anthracnose and brown blotch, and has important practical significance on prevention and treatment of citrus anthracnose and brown blotch. And the biological agent is environment-friendly and non-toxic in source, has small influence on the ecological environment, and has a relatively good application prospect.

The present application provides a method for raising seedlings of honeysuckle by root cuttings. The method comprises the following steps: (1) selecting healthy, pest-free, and 0.3 cm thick root strips from a mother tree as cuttings, cutting the root strips into 5-8 cm long strips and disinfecting them; (2) after disinfection, quickly dipping the base of the cuttings with a plant rooting agent, and then soaking the cuttings in a composite nutrient solution comprising the following raw materials: loofah extract, Ailanthone, Acanthopanax senticosus polysaccharide, and ethanol; taking the cuttings out after soaking for 10-15 minutes before cutting; (3) spraying the cuttings alternately with a composite agent and a fungicide every 5-7 days after cutting, at night when the leaf surface is free of water, wherein the composite agent is prepared by extracting gentian, akebia, and kudzu root. The method of the present invention effectively increases the growth rate of honeysuckle seedlings, improves disease resistance to diseases such as brown spot disease, white rot, powdery mildew, etc., promotes healthy and rapid growth, and improves seedling survival rate and seedling quality.

The invention belongs to the technical field of microorganisms, and discloses a pleurotus purpureus strain 162 and a cultivation method thereof. The preservation number of the strain 162 is CGMCC (China General Microbiological Culture Collection Center) No. 42041. The cultivation method comprises the following steps: 1) inoculating a pleurotus purpureus strain 162 on a mother strain culture medium to obtain a strain 162 mother strain; 2) inoculating the strain 162 mother strain to a stock culture medium to obtain a strain 162 stock; 3) inoculating the strain 162 stock seed to a cultivar culture medium to obtain a strain 162 cultivar; and 4) cultivating the cultivar of the strain 162 to obtain the sporocarp of the strain 162. The strain 162 is fast in hypha growth, short in after-ripening period, fast in fruiting, white in pileus, strong in adaptive capacity and short in harvesting period; the biological efficiency is high; the feed is high in nutritional value and has strong disease resistance to bacterial brown spot.

The present invention relates to the technical field of microbial control, and discloses a Bacillus velezensis nanomaterial composite microbial agent, a preparation method thereof and an application. The microbial agent of the present invention is prepared by the following steps: preparing a bacterial suspension of Bacillus velezensis; after mixing sodium alginate with the bacterial suspension, adding nano-carbon and stirring to obtain a mixed solution; adding the mixed solution into a stirred calcium chloride solution, and reacting to obtain a Bacillus velezensis microcapsule microbial agent. The microbial agent of the present invention has an antibacterial rate of >56% against Citrus brown spot pathogen, Citrus anthracnose pathogen, Citrus greasy spot and yellow spot pathogen, Banana wilt pathogen, Banana long-shaped spot pathogen; the control effect on Huanglongbing reaches 57.84%. After being irradiated by ultraviolet rays, the antibacterial ability of the microbial agent of the present invention only decreases by 4.32%, and it can play a biological control role for a long time. The growth amount of citrus seedlings treated with the microbial agent of the present invention can be increased by 220.39%, and the number of newly grown leaves can be increased by 244.67%, having a good plant growth promotion effect.

The invention belongs to the technical field of biological detection, and particularly relates to a primer group and a kit for detecting new plum brown spot and application of the primer group and the kit, and base sequences of the primer group are shown as SEQ ID NO.1-SEQ ID NO.5. Based on the primer group, the loop-mediated isothermal amplification technology is used for rapid detection of the alternaria nepalensis, and the alternaria nepalensis can be accurately detected from a complex pathogenic bacterium environment in diseased plant tissues. The rapid detection system disclosed by the invention is good in specificity and high in sensitivity, and the detection limit of the DNA of the alternaria nepalensis is 0.246 fg / mu L. By utilizing the rapid detection system provided by the invention, the alternaria nepalensis can be rapidly, simply, accurately and sensitively detected from a field sample within 45 minutes, and compared with a traditional symptom recognition method and a common molecular detection method, the rapid detection system is simpler and more efficient.

The invention relates to a rice disease detection method based on a YOLO algorithm, in particular to high-precision detection achieved by improving a YOLOv11 model. The method combines a plurality of innovative modules, and aims to improve the detection accuracy of rice diseases (such as bacterial leaf spot disease, brown spot disease and leaf tumor disease). By introducing a CPA-Enhancer image enhancement module, a bidirectional feature pyramid network (BiFPN), a CARAFE content perception feature recombination module and a CBAM double attention mechanism, the small target identification problem of rice leaf scab can be effectively solved, and the challenges of background interference and multi-scale feature fusion in a complex field environment are overcome. According to the method, through multi-stage optimization, the recognition precision and the detection speed of the model on rice diseases are remarkably improved, and compared with a traditional method, the method has obvious advantages in the aspects of small target detection and scab boundary detail recovery. Experimental results show that the model of the invention is superior to the existing YOLO series and other common deep learning models in multiple performance indexes, and is suitable for large-scale field real-time monitoring and intelligent agriculture application.

The application discloses a pyruvate dehydrogenase E2 inhibitor and a preparation method thereof. The application also discloses a bactericide containing the pyruvate dehydrogenase E2 inhibitor. The application occupies the binding site of lipoic acid in the active cavity by designing an analogue of lipoic acid, so as to hinder the catalytic reaction and achieve the purpose of inhibiting the growth of organisms. Based on this, the application takes the pyruvate dehydrogenase system in microorganisms as a target, designs and synthesizes a novel and efficient pyruvate dehydrogenase E2 (PDHc-E2) inhibitor, which can be used for developing a novel and efficient bactericide and is widely applied to the prevention and treatment of bacterial brown spot bacteria of rice or bacterial leaf blight bacteria of rice.

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to application of streptomyces aureoflavus in prevention and treatment of beet brown spot. The invention provides an application of more than one of the following products in prevention and treatment of beet brown spot and / or beet root rot: (1) Streptomyces aureoflavus, (2) Streptomyces aureoflavus, (3) Streptomyces sp., (4) Streptomyces sp., (5) Streptomyces sp., (6) Streptomyces sp., (6) Streptomyces sp., (7) Streptomyces sp. The preservation number of the streptomyces aureoflavus is CGMCC (China General Microbiological Culture Collection Center) No. 28506, and the preservation number of the streptomyces aureoflavus is CGMCC No. 28506. (2) preparing a fermentation supernatant of streptomyces aureoflavus; (3) preparing a fermentation culture of streptomyces aureoflavus; and (4) preparing an extract of a fermentation supernatant of streptomyces aureoflavus. A pot experiment shows that the yield of the beet can be reduced by 42.585% due to the occurrence of the beet brown spot, and the biocontrol strain CYS4-5 can promote the yield of the beet to be increased by 46.122% while preventing and treating the beet brown spot disease.

The present application relates to a bacillus velezensis strain JZ01, a microbial agent containing the same and application thereof. The bacillus velezensis strain JZ01 can efficiently antagonize the pathogen of cucumber brown spot and effectively kill root-knot nematodes. Further, the present application also develops a synergist of the strain. It is found that trigonelline can be used as a synergist to significantly increase the efficacy of the strain against the pathogen of cucumber brown spot, and ganoderic acid A can enhance the efficacy of the strain in killing root-knot nematodes, so that a more efficient compound microbial agent can be developed. In addition, the bacillus velezensis strain and the microbial agent containing the same are microbial agents, which are safe and environmentally friendly, and therefore have high industrial value and application prospect.

The invention provides a trichoderma inoculant, a preparation method thereof and application of the trichoderma inoculant in improving yield and quality of beet, and belongs to the technical field of agricultural microbial inoculants. The trichoderma agent comprises the following components: trichoderma harzianum, trichoderma viride, purpureocillium lilacinum, bacillus amyloliquefaciens and bacillus laterosporus in a mass ratio of (3-5): (1-3): (2-4): (2-4): (1-2). According to the invention, the strains cooperate with each other to promote the growth of beet, effectively enhance the immune resistance of beet, and effectively prevent and reduce beet root rot, brown blotch and other diseases caused by continuous cropping; the photosynthesis efficiency of the beet can be enhanced, the accumulation of photosynthetic products is improved, the soil structure is improved, the number of beneficial bacteria groups in the soil is increased, and the rhizosphere environment is improved, so that the sugar content and yield of the beet are increased. By applying the microbial agent disclosed by the invention, multiple effects of increasing the yield per mu, increasing the sugar content of beet, improving soil, improving the fertilizer efficiency, resisting diseases, preventing insects and the like can be achieved, and farmers are helped to increase both production and income.

The invention provides an antagonistic bacterium PG-NS-24b strain for brown spot of oyster mushroom, the classification name of the antagonistic bacterium PG-NS-24b strain is Delftia acidovorans, the antagonistic bacterium PG-NS-24b strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.34764. The antagonistic bacterium PG-NS-24b strain can be used for preventing and treating brown spot of oyster mushroom. The PG-NS-24b strain is found to have a good antagonistic effect on the brown spot of the oyster mushrooms for the first time, so that a solid foundation is laid for screening of biocontrol strains of the oyster mushrooms and biological prevention and control of diseases, and the PG-NS-24b strain plays a role in promoting development of safe and efficient biopesticide preparations, and has important theoretical significance and practical value. The result shows that the treatment effect of the PG-NS-24b strain is 23.12%, and the prevention effect of the PG-NS-24b strain is 59.06%. In conclusion, the PG-NS-24b strain has the biocontrol potential of preventing and treating the brown spot of the oyster mushrooms.

The application discloses a method for detecting mulberry brown spot based on a mitochondrion cob gene. Specifically, the method comprises a mitochondrion cob gene of Neophloeospora maculans and application of the mitochondrion cob gene in detection of mulberry brown spot and / or Neophloeospora maculans, a primer group for detecting Neophloeospora maculans, a kit, a method and application thereof. The primer group for specifically detecting Neophloeospora maculans can be combined with a conservative region of a target gene in a specific manner, and the PCR reaction has high selectivity, can be sensitively detected when a small amount of pathogenic bacteria exists, and increases the accuracy and sensitivity of detection. The method is rapid and simple, is suitable for detection under laboratory and field conditions, helps to early detect diseases, and timely takes corresponding prevention and control measures, and reduces the influence of diseases on the yield and quality of mulberry.

The present invention relates to a Bacillus Velezii ( Bacillus from Velez ) HMF10, whose deposit number is CGMCC No. 30476. This strain can effectively antagonize rice stem tip nematode and Pseudomonas syringae pv. syringae, and can effectively prevent and control rice stem tip nematode disease and rice bacterial brown spot disease.

The invention provides a composition for preventing and treating alfalfa brown spot as well as a preparation, a preparation method and application thereof, and belongs to the technical field of biopesticides. The composition for preventing and treating the alfalfa brown spot disease is prepared from the following components in parts by mass: 14 to 22 parts of eugenol, 6 to 10 parts of berberine and 25 to 40 parts of gallnut extract. The invention further provides wettable powder for preventing and treating the alfalfa brown spot disease. The wettable powder comprises the following components in percentage by mass: 20-80% of the composition, 3-5% of a wetting agent, 6-9% of a dispersing agent and the balance of filler. Field control effect test results show that the composition has a synergistic effect on the control effect of the alfalfa brown spot, and compared with a single effective component, the composition can improve the control effect on the alfalfa brown spot. In addition, the active ingredients are all plant ingredients, green and pollution-free, and can be popularized and applied in a large range.

The invention belongs to the technical field of microorganisms, and particularly discloses a method for rapidly separating single spores of camellia oleifera brown spot bacteria. Comprising the following steps: collecting a sample; the method comprises the following steps: selecting scab tissues with obvious characteristics (symptoms) on a sample, slicing in a bare-handed manner, placing a slice material on a glass slide, and dropwise adding sterile water to prepare a bacterial spore-containing mixed solution; the method comprises the following steps: taking a glass sample application capillary tube with the specification of 0.3 * 100mm, firing the middle part of the capillary tube on the outer flame of an alcohol lamp, then lengthening the capillary tube until the capillary tube is broken within 3-5 seconds, cutting off the fired top end by using scissors, and inserting the unfired end into a gun head of a gene gun with the specification of 10-100 microliters; aligning a single spore in the mixed solution under a stereoscope, and touching and sucking the single spore; installing a gene gun on the head of the gene gun into which the monospores are sucked, and rapidly scribing and striking the sucked monospores into a PDA culture medium; culturing in a constant-temperature box; with the adoption of the technical scheme, the camellia oleifera brown spot fungus monospore can be directly obtained, the operation process is simple, the period is short, the success rate is high, and the method is applicable to treatment of large-batch samples.

The invention discloses a sanguinarine sterilization composition containing a macleaya cordata extract and application of the sanguinarine sterilization composition. The sanguinarine sterilization composition mainly comprises sanguinarine and pydiflumetofen. The two components do not have cross resistance, the two medicaments have obvious synergistic effect after being combined according to a certain ratio, and a common agricultural bactericide dosage form is prepared by adding a proper amount of agricultural bactericide auxiliaries and has higher control effect on fungal diseases of crops. Through combined use of the formula, the dosage can be reduced, the drug resistance of germs to a single agent can be delayed, ecological damage and environmental pollution are effectively reduced, and the yield and quality of crops can be improved; the composition can efficiently prevent and control crop sickle root rot, increases a disease prevention spectrum, and can prevent common crop diseases such as anthracnose, leaf spot, early blight, brown blotch and gray mold at the same time.

The invention belongs to the field of molecular genetic breeding, and particularly relates to a group of molecular markers related to disease resistance of apple brown blotch, a probe group and application, the molecular markers comprise four SNP markers and three InDel markers, through combination of the two molecular markers, four disease resistance grades of high brown blotch resistance, brown blotch resistance, moderate brown blotch infection and brown blotch infection can be accurately divided, and the disease resistance of the apple brown blotch is greatly improved. Therefore, auxiliary selection is provided for apple breeding.

The invention belongs to the technical field of pesticide compounding, and particularly relates to a bactericidal composition containing sulfatrione and benziothiazolinone and application of the bactericidal composition. When the mass ratio of the effective components, namely, the prosulfuron (A) and the benziothiazolinone (B) is (1: 25)-(25: 1), the action mechanisms of the two effective components are complementary, synergistic interaction is achieved, the obvious control effect on wheat leaf blight, sclerotinia rot of colza, watermelon leaf blight and beet brown spot is achieved, and the lasting period is long.

This invention discloses a method and system for identifying rice leaf diseases based on an improved ResNet18 model, belonging to the fields of agricultural artificial intelligence and computer vision technology. The method uses ResNet18 as the backbone network and innovatively embeds convolutional block attention modules (CBAM) serially at the feature output of each residual basic unit. It utilizes a dual channel and spatial attention mechanism to recalibrate the feature map, effectively suppressing background noise and focusing on minute lesion features. Simultaneously, it constructs a cross-entropy loss function (L2-CE Loss) with L2 norm regularization constraints, improving training stability and generalization ability by constraining the magnitude of the output layer Logits vector. Experiments show that the method of this invention achieves an average identification accuracy of 99.67% for rice bacterial blight, rice blast, brown spot disease, and healthy leaves under complex backgrounds, significantly outperforming the traditional ResNet18 model. It exhibits higher generalization ability and robustness, making it suitable for real-time disease monitoring in the field.

The invention discloses a biological prevention and control preparation for leaf brown blotch of malus asiatica and application of the biological prevention and control preparation, and relates to the technical field of agricultural disease prevention and control, and the prevention and control preparation comprises the following active ingredients: bacillus subtilis, trichoderma, actinomycetes, potassium silicate and brassinos.The biological prevention and control preparation for leaf brown blotch is compounded by adopting biological active ingredients; lipopeptide antibiotics generated by the bacillus subtilis destroy pathogen cell membranes, the trichoderma directly digests hyphae through hyperparasitism, the actinomycetes secrete antibiotics to interfere pathogen cell wall synthesis and promote trichoderma colonization, and the three forms an inhibition-digestion-strengthening three-dimensional antibacterial network. Potassium silicate forms a nanoscale siliceous layer on plant epidermis, a plant defensive enzyme system is activated to form physical and biochemical dual protection, and brassin enhances the plant disease-resistant compensation capability by promoting chlorophyll synthesis and photosynthetic efficiency.

The invention provides an antagonistic bacterium P3 strain for brown spot of oyster mushroom, the classification name of the antagonistic bacterium P3 strain is bacillus siamensis, the antagonistic bacterium P3 strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.34763. The antagonistic bacterium P3 strain has the advantages that the antagonistic bacterium P3 strain can be used for resisting brown spot of oyster mushroom; the P3 strain is found to have a good antagonistic effect on the brown spot of the oyster mushrooms for the first time, so that a solid foundation is laid for screening of biocontrol strains of the oyster mushrooms and biological prevention and control of diseases, and the P3 strain plays a role in promoting development of safe and efficient biopesticide preparations and has important theoretical significance and practical value. The result shows that the treatment effect of the P3 strain is 24.12%, and the prevention effect is 56.57%. In conclusion, the P3 strain has the biocontrol potential of preventing and treating the brown spot of the oyster mushrooms.

The invention relates to a diselenide compound and application of the diselenide compound in preparation of pesticides and medicines, in particular to application of the diselenide compound in preparation of plant virus resisting agents and agricultural bactericides, and application of the diselenide compound in preparation of anti-cancer medicines, especially medicines for resisting lung adenocarcinoma, liver cancer, pancreatic cancer and breast cancer. The diselenide compound disclosed by the invention has a very good prevention effect on tobacco mosaic virus (TMV) under the concentration of 500mg / L, and the living body passivation prevention effect can reach 55.0%. The diselenide compound disclosed by the invention has a very good prevention and treatment effect on tomato early blight, wheat scab, rice blast, phytophthora capsici, sclerotinia rot of colza, cucumber gray mold, rice sheath blight disease, cucumber fusarium wilt, peanut brown spot, apple ring spot, wheat sharp eyespot, corn southern leaf blight, watermelon anthracnose and rice bakanae disease at the concentration of 50mg / L; and the bacteriostatic activity on the rice blast can reach 100%. The diselenide compound disclosed by the invention has a very strong inhibition effect on various cancer cells, and the IC50 of the diselenide compound on pancreatic cancer cells can reach 4.33 mu M.

The invention relates to a bacillus velezensis strain JZ01, a fungicide containing the same and application of the bacillus velezensis strain JZ01. The bacillus velezensis strain JZ01 can be used for efficiently antagonizing cucumber brown spot pathogen cercospora, and effectively killing root-knot nematode. Furthermore, the invention also develops a synergist of the bacterial strain, and the research finds that the trigonelline can be used as the synergist to significantly increase the effect of the trigonelline on resistance to cephalosporium, while the ganoderic acid A can enhance the effect of the trigonelline on killing root-knot nematode, so that the trigonelline can be used for developing a more efficient compound bacterial agent. Besides, the bacillus velezensis strain and the fungicide containing the bacillus velezensis strain are microbial preparations and have the advantages of being safe and environmentally friendly, and therefore the bacillus velezensis strain and the fungicide containing the bacillus velezensis strain have high industrial value and application prospects.

The present invention relates to an asymmetric aromatic diselenide compound and the use thereof in the preparation of pesticides, particularly to the use thereof in the preparation of anti-plant virus agents and fungicides. The asymmetric aromatic diselenide compound of the present invention exhibits a good efficiency on tobacco mosaic virus (TMV) at a concentration of 500 mg / L, achieving an in-vivo inactivation efficiency of 71.6%, an in-vivo protective efficiency of 58.3%, and an in-vivo therapeutic efficiency of 60.1%. The asymmetric aromatic diselenide compound of the present invention exhibits good control effects on tomato early blight, wheat Fusarium head blight, rice blast, pepper Phytophthora blight, rapeseed Sclerotinia rot, cucumber gray mold, rice sheath blight, cucumber wilt, peanut brown spot, apple ring rot, wheat sharp eyespot, southern corn leaf blight, watermelon anthracnose, and rice bakanae disease at a concentration of 50 mg / L, and has a fungicidal activity of up to 92.3%.