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Application of cytokine activin C in treatment of neuropathic pain

A cytokine and activin technology, applied in the field of biomedicine, can solve problems such as unclear role

Pending Publication Date: 2021-01-08
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of activin C in neuropathic pain remains unclear

Method used

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  • Application of cytokine activin C in treatment of neuropathic pain
  • Application of cytokine activin C in treatment of neuropathic pain
  • Application of cytokine activin C in treatment of neuropathic pain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one, experiment preparation

[0030] 1. Source of experimental animals:

[0031] In the present invention, animals and animal studies were performed in accordance with the recommendations set forth in the Arrival Guidelines and the British Journal of Pharmacology. All experiments were performed in accordance with the guidelines of the International Association for Pain Research and approved by the Experimental Animal Use Committee of Nantong University. To avoid confounding effects of estrous cycle on pain processing, only male animals were used in this study.

[0032] 1-1. Adult male SD rats (200-250 g), young male SD rats (90-110 g) and 8-12-week-old male C57 / BL mice were provided by Shanghai SLAC Experimental Animal Company.

[0033] 1-2. TRPV1 knockout (KO) mice were purchased from Jackson Laboratory in Bar Harbor, Missouri, USA, and crossed with adult female C57BL / 6J wild-type (WT) mice.

[0034] 1-3. Conduct behavioral tests with male TRPV1 KO and WT...

Embodiment 2

[0045] Embodiment two, experimental method

[0046] 1. Sciatic nerve cut, autotomy scoring and dorsal root ganglion isolation

[0047] The sciatic nerve of adult rats was deeply anesthetized with 3% pentobarbital sodium (80 mg / kg). The left sciatic nerve was transected in the mid-thigh, cutting the nerve (1.0 cm). Experimental animals were anesthetized with 0.1 M phosphate cold buffer solution (PBS, pH 7.4), perfused through the left ventricle, and L4~5DRG were rapidly isolated under ribonuclease-free conditions at 0.5, 1, 2, 7, 14, and 28 days after operation , and served as a complete control on the 0th day after operation (n = 9 / time point).

[0048] For axotomy scoring, each rat was scored daily for the level of autotomy for four weeks, which was modified from the prior art in a double-blind manner. The following rule was used: 0.1 points for the loss of 1 nail or 1 bleeding finger, and 1 point for each distal half-finger attacked. Then, add one point for each proximal...

Embodiment 3

[0079] Example 3. Up-regulation of the expression of activin C in the lumbar dorsal root ganglion of rats with peripheral nerve injury

[0080] In order to determine the dose of activin C injected intrathecally into adult rats, the present inventors firstly studied the expression of this cytokine in the lumbar dorsal root ganglion of chronic neuropathic pain rats.

[0081] In this example, rat sciatic nerve was cut, which is a simple and feasible peripheral nerve injury model. A custom microarray was used (Liu et al., 2012).

[0082] figure 1 In the present invention, after the rat sciatic nerve is cut off, the expression of activin βC subunit and activin C protein in the lumbar 4-5 dorsal root ganglion (DRG) is up-regulated. in, figure 1 a is a graph of custom microarray results, N = 6 separate experiments; figure 1 b is the real-time RT-PCR analysis graph, N = 6 separate experiments; figure 1 c is the western blot display, activin C at 25 kDa is composed of βC and βC su...

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Abstract

The invention discloses application of cytokine activin C in inhibiting neuropathic pain and application of the activin C in preparation medicines for treating neuropathic pain, and discloses a medicine for treating neuropathic pain. The medicine at least comprises activin C. The peripheral nerve injury induced neuropathic pain model prepared in the invention verifies that endogenous activin C isremarkably expressed and remarkably up-regulated in small neurons of dorsal root ganglion (DRG), and chronic neuropathic pain can be inhibited by adjusting TRPV1 channels if the activin C is applied in peripheral local and intrathecal parts, and the potential of the activin C for treating chronic neuropathic pain can be prompted.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and relates to the application of activin C in treating neuropathic pain, in particular to the application of activin C in inhibiting neuropathic pain, the application of activin C in preparing medicines for treating neuropathic pain, and a A drug for the treatment of neuropathic pain. Background technique [0002] Chronic neuropathic optic neuralgia, caused by somatosensory nervous system disorders or specific disorders, affects a large proportion of the population and has potentially negative consequences for patients. Currently, the underlying mechanisms of neuropathic pain are unclear. [0003] Often, existing treatments do not work well for patients with neuropathic pain. Therefore, new therapeutic drugs or targets must be explored to improve the therapeutic effect. [0004] Accumulating evidence indicates that cytokine signaling plays a key role in chronic neuropathic pain processing...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61P25/02A61P29/00
CPCA61K38/1841A61P25/02A61P29/00
Inventor 刘兴君陈勇
Owner NANTONG UNIVERSITY
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