A kind of mineralization conditioner of phosphate solubilizing bacteria and its preparation method and repairing and improving method of heavy metal polluted soil

A technology of phosphate solubilizing bacteria and conditioner, applied in the field of microbial remediation, can solve the problems of occupying cultivated land environment, pollution, etc., and achieve the effects of improving soil acidification, broad market prospects, and low production costs

Active Publication Date: 2022-04-26
FUJIAN LONGKING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, there are still a large amount of phosphate slag being dumped, which not only occupies a large amount of arable land but also causes serious pollution to the environment

Method used

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  • A kind of mineralization conditioner of phosphate solubilizing bacteria and its preparation method and repairing and improving method of heavy metal polluted soil
  • A kind of mineralization conditioner of phosphate solubilizing bacteria and its preparation method and repairing and improving method of heavy metal polluted soil
  • A kind of mineralization conditioner of phosphate solubilizing bacteria and its preparation method and repairing and improving method of heavy metal polluted soil

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preparation example Construction

[0032] The present invention also provides a kind of preparation method of phosphate solubilizing bacteria mineralization conditioning agent, comprises the following steps:

[0033] The phosphate-dissolving bacteria agent is mixed with the phosphate rock slag powder to obtain the phosphate-dissolving bacteria mineralization conditioner;

[0034] The phosphate-solubilizing bacteria in the phosphate-solubilizing bacteria agent include Penicillium pumilus and Aspergillus japonicus.

[0035] In the preparation method provided by the present invention, the phosphate-dissolving bacteria agent and the phosphate rock slag powder are directly mixed uniformly in proportion to obtain the phosphate-dissolving bacteria mineralization conditioner provided by the present invention. Wherein, the phosphate solubilizing bacteria agent is preferably prepared according to the following steps:

[0036] The phosphorus-solubilizing bacteria fermented liquid is adsorbed and mixed with the carrier, a...

Embodiment 1

[0057] Prepare the phosphate-dissolving bacteria mineralization conditioner, the specific preparation steps are as follows:

[0058] (1) Acquisition of strains: use polluted soil to prepare soil suspension, separate, screen and purify it through inorganic phosphorus solid medium; wherein, the composition of inorganic phosphorus solid medium is glucose: 10g / L, Ca 3 (PO 4 ) 2 : 5g / L, (NH 4 ) 2 SO 4 : 0.5g / L, MgSO 4 ·7H 2 O: 0.3g / L, NaCl: 0.3g / L, KCl: 0.3g / L, FeSO 4 ·7H 2 O: 0.03g / L, MnSO 4 ·H 2 O: 0.03g / L, agar: 20g / L, the balance is water, and the pH value is 7.0; the culture temperature is 30°C, the rotation speed is 150rmp / min, and the time is 5 days; The strains with strong phosphorus-solubilizing ability were initially screened by none and size, and finally after five generations of purification, the phosphorus-solubilizing bacteria were obtained, namely Eupenicillium parvum and Aspergillus japonicus.

[0059] (2) Fermentation broth culture: Inoculate appropriate...

Embodiment 2

[0065] Effects of Different Dosage of Phosphorus Solubilizing Bacteria Mineralization Conditioner on Soil pH

[0066] The soil to be tested was collected from a farmland soil with light or moderate heavy metal pollution in Longyan. The sampling depth was 0-20 cm, and it was air-dried in a cool, non-polluted place. content of heavy metals. The heavy metal polluted soil is a compound polluted soil containing Pb and Cd, and the contents of each heavy metal are shown in Table 1:

[0067] Table 1 Heavy metal content in heavy metal polluted soil

[0068]

[0069] The mass ratio of the phosphorus-dissolving bacteria agent prepared in Example 1 and the phosphate rock slag powder is the phosphorus-dissolving bacteria mineralization conditioning agent of 1:3, and different phosphorus-dissolving bacteria mineralization conditioning agent dosages (conditioning agent dosage) are set. 0.1%, 0.5%, 1%, and 2% of the soil mass respectively), the heavy metal-contaminated soil and the phosp...

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Abstract

The invention belongs to the technical field of microbial remediation of polluted environment, and in particular relates to a mineralization conditioner for phosphate-solubilizing bacteria, a preparation method thereof, and a method for remediation and improvement of heavy metal-contaminated soil. The phosphate-solubilizing bacteria mineralization conditioner provided by the present invention includes a phosphate-solubilizing bacteria agent and a phosphate rock slag powder; the phosphate-solubilizing bacteria in the phosphate-solving bacteria agent include Penicillium pumilus and Aspergillus japonicus. The conditioner provided by the invention not only realizes the resource utilization of phosphate slag, but also simultaneously realizes the improvement of soil available phosphorus content and the mineralization of soil available Pb and Cd by using phosphate-dissolving bacteria agent to compound phosphate slag powder And the improvement of soil acidification, and will not cause secondary pollution to the environment. The raw material of the conditioning agent is cheap and easy to obtain, the production cost is low, the effect of repairing and improving soil polluted by heavy metals is good, and the market prospect is very broad.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of polluted environment, and in particular relates to a mineralization conditioner for phosphate-solubilizing bacteria, a preparation method thereof, and a method for remediation and improvement of heavy metal-contaminated soil. Background technique [0002] Soil is the cornerstone for the survival and development of human beings, and an important material basis for ensuring safe food production. At present, my country's farmland soil is facing a variety of problems, among which the soil is polluted by heavy metals, and the problems of insufficient soil fertility and soil acidification are the most prominent. Although traditional physical remediation and chemical remediation methods can quickly remediate heavy metal-contaminated soil in a short period of time, they cannot simultaneously solve the problems of soil fertility decline and acidification. However, traditional soil conditi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/40C09K109/00C09K101/00
CPCC09K17/40C09K2101/00C09K2109/00
Inventor 代双艳肖尧钟安良陈俊华周志威郭丹花
Owner FUJIAN LONGKING
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