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Method and system for oxidizing L-cysteine to generate L-cystine

A cysteine ​​and cystine technology, which is applied in the field of L-cysteine ​​oxidation to generate L-cystine, can solve the problems of long equipment occupation, low production efficiency, long waiting time, etc., and achieve the detection time. Short, low cost, easy to operate effect

Active Publication Date: 2021-02-02
湖北远大生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The specificity of this method is good, and the content of L-cysteine ​​can still be accurately detected under the coexistence of sulfhydryl compounds and other α-amino acids. However, due to the high price of HPLC instruments, the time taken by the instrument is often long when there are many samples. In addition, the waiting time for the detection of multiple samples also caused a long waiting time for the oxidation process, and the production efficiency was extremely low

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  • Method and system for oxidizing L-cysteine to generate L-cystine

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Embodiment 1

[0050] The enzymatic reaction liquid containing L-cysteine ​​is passed into the reaction tank, and air is passed through, and the L-cysteine ​​is oxidized under the action of oxygen to generate L-cystine. Relevant data of different batches of different processes were detected by redox electrode potential measuring instrument and high performance liquid chromatography, and the data of cysteine ​​concentration less than 0.5g / L were sorted out in Table 1 below.

[0051] Table 1 Comparison table of cysteine ​​detection data and potential data during the oxidation process of different batches of samples

[0052] batch number Cysteine ​​(g / L) Potential value (MV) 2006302 0.28 -217 2006286 0.06 -235 2006289 0.12 -242 2006250 0.27 -259 2006283 0.13 -263 2006308 0.01 -263 2006296 0.18 -268 2006288 0.16 -268 2006278 0.23 -269 2006309 0.06 -269 2006267 0.18 -276 2006254 0.25 -277 2006300 0.30 -286 ...

Embodiment 2

[0057] The enzymatic reaction liquid containing L-cysteine ​​is passed into the reaction tank, and air is passed through, and the L-cysteine ​​is oxidized under the action of oxygen to generate L-cystine. Relevant data of different processes of different batches were detected by redox electrode potential measuring instrument and high-performance liquid chromatography, and the data of sorting potential values ​​less than -300MV are shown in Table 2 below.

[0058] Table 2 Contrast Table of Cysteine ​​Concentration and Potential Value (P<-300MV) in Different Batches of Samples

[0059] batch number Cysteine ​​concentration (g / L) Potential value (MV) 2007046 0.11 -372 2007044 0.12 -364 2007033 0.17 -358 2007026 0.18 -353 2006206 0.21 -303 2006383 0.29 -343 2006305 0.37 -336 2006299 0.38 -328 2006294 0.43 -319 2006279 0.57 -316 2006261 0.68 -309 2006259 1.10 -306 2007047 1.10 -382 200704...

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Abstract

The invention provides a method and system for generating L-cystine by oxidizing L-cysteine. The method comprises the following steps: 1, introducing oxygen-containing gas into a solution containing L-cysteine, and measuring the potential value P of the solution; 2, judging the relation between the potential value P and a preset potential value P1, and when the potential P is larger than or equalto P1, stopping oxygen-containing gas from being introduced; and 3, collecting separated-out L-cystine. The method and the system provided by the invention adopt potential value detection to control the reaction endpoint, so that the traditional HPLC detection required in the whole process to control the reaction endpoint is replaced, and the method and the system are convenient to operate and short in detection time, can fully oxidize the L-cysteine in the solution to generate the L-cystine, improve the yield of the product and the utilization efficiency of equipment, are simple, convenient and quick in process control operation and low in cost, and are suitable for scale industrial production.

Description

technical field [0001] The invention relates to the field of biomedicine. In particular, the present invention relates to methods and systems for the oxidation of L-cysteine ​​to L-cystine. Background technique [0002] L-cysteine, chemical formula C 3 h 7 NO 2 S, discovered in the form of cystine from bladder stones in 1810, the chemical name is L-α-amino-β-mercaptopropionic acid, which often exists in the form of oxidized L-cystine in proteins, is 20 species Among the natural amino acids, it is the only amino acid that contains hydrogen sulfhydryl groups in the branched chain. These sulfhydryl groups are extremely chemically active, and the disulfide bonds formed can greatly improve the stability of proteins. L-cysteine ​​has a wide range of properties and functions. It breaks disulfide bonds in peptides and proteins, acts as a free radical scavenger, and acts as a reducing agent. [0003] L-cystine, chemical formula C 6 h 12 N 2 o 4 S 2 , is one of the essentia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C319/24C07C323/58G01N27/26G01N30/02
CPCC07C319/24C07C323/58G01N27/26G01N30/02C07B2200/07G01N2030/027
Inventor 赵东明曹亚杰张静林添雄欧阳晖
Owner 湖北远大生物技术有限公司
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