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Halophilic archaea for degrading nitrite and application of halophilic archaea

A technology of halophilic archaea and nitrite, applied in the field of bioengineering, can solve problems such as not being screened

Active Publication Date: 2021-02-09
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The technical effect of this new technology was that it improved upon previous methods by making them more effective at producing certain compounds or substances from raw materials like sugar without generating harmful side products during production process.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving salty food safety while also minimizing harmful effects on human health caused by excessive levels of nitrites (NO) from nitric oxide (N02). Nitrite is formed when plants use water containing calcium cations like Ca(OH), Fe(III)). It forms crystals called calcite upon exposure to air at temperatures above 50°C. These crystal structures contain ammonium ion (Na+) and other elements such as iron sulfides. Toxic gases released during decomposition produce hydrochloric acids, causing damage to fishing nets used for transporting seafoods. Additionally, nitride generated during processing causes environmental pollution if dumped overboard after harvesting. Therefore, there needs methods to remove these unwanted substances without affecting beneficial nutrients found naturally within the plant itself.

Method used

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  • Halophilic archaea for degrading nitrite and application of halophilic archaea
  • Halophilic archaea for degrading nitrite and application of halophilic archaea

Examples

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Effect test

Embodiment 1

[0019] Example 1: Screening of Halophilic Archaea

[0020] Take the farmhouse's homemade salted mustard lumps sold in the farmers' market in Anda City, Heilongjiang as the sample, weigh 10g of the sample, add 90mL of normal saline after crushing, fully shake for 1h and then let stand for 2h, take the supernatant for 10-fold gradient dilution to 10 -1 ~10 -7 , 100 μL of each was applied to NHM solid medium, and after culturing in a 37°C incubator for 7 days, single colonies with pink, round, neat edges were selected and streaked three times on NHM solid medium to obtain pure colonies. The strain JB33.

Embodiment 2

[0021] Example 2: Preliminary identification of denitrification function of halophilic archaea

[0022] The screened strain JB33 was activated in NHM medium, inoculated into a screw-cap tube (Durham tube) of liquid denitrification medium with 1% inoculum, and cultured at 37°C to observe whether the screw-cap tube produced gas. , to test whether the strain can metabolize nitrite to produce gas. It was observed that after culturing for 7 days, there were bubbles in the screw cap tube, indicating that the strain JB33 could metabolize nitrite to generate gas.

Embodiment 3

[0023] Example 3: Morphology and molecular biology identification of halophilic archaea

[0024] The strain JB33 was inoculated into solid NHM medium and cultured at 37°C for 7 days. After a single colony was grown, the morphological characteristics and color of the colony were observed, and then a single colony was picked for Gram staining: smear, fixation, crystal violet After primary staining, iodine mordant staining, ethanol destaining, water washing, and safranine counterstaining, drying and microscopy were performed. Its biological characteristics are: Gram staining negative, the colonies on the NHM plate are pink, round, translucent, with neat edges.

[0025] According to conventional methods, genomic DNA was extracted from the pure culture of strain JB33 for molecular biological identification: PCR amplification was carried out with the strain 16S rDNA sequence as the template. The upstream primer for PCR amplification is 20F, and its nucleotide sequence is shown in S...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to halophilic archaea for degrading nitrite and an application of the halophilic archaea. According to the invention, a new species of Haloarcula is found, and the new species is named as Haloarcula sp. JB33. The strain is aerobic denitrifying bacteria, can grow within the salt concentration range of 5-25% andthe temperature range of 20-52 DEG C, and has good salt tolerance. The optimal growth temperature is 37 DEG C. When the salt concentration is 18% and the nitrite concentration is 100mg/kg-500mg/kg, the strain still normally grows and effectively degrades nitrite, and the optimal degradation rate reaches 98.59% after the strain is degraded for 96 hours. Nitrite degradation effect is obvious. The strain can be used for degrading the nitrite in foods. The halophilic archaea is particularly applied to degradation of the nitrite in high-salt pickled foods such as pickled vegetables and hams.

Description

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Claims

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Application Information

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Owner JIANGSU UNIV
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