Bioreactor and application thereof
A technology of bioreactor and cone, which is applied in the direction of bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical instrument, etc. It can solve the problems of low oxygen transfer efficiency and small operating range, and achieve Low risk of bacterial contamination, simplified design, and easy control
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Embodiment 1
[0064] combined with figure 1 And attached figure 2 , the present embodiment provides a bioreactor, including a main body 101, the main body 101 includes a first cone 102, a cylinder 103 and a second cone 104, the first cone 102, cylinder 103 and second The cones 104 are sequentially connected along the height direction of the main body 101, the first cone 102 is located at the top of the main body 101, the second cone 104 is located at the bottom of the main body 101, and the first cone 102 and the second cone 102 are located at the top of the main body 101. The tips of the two cones 104 all face away from the cylinder 103; wherein:
[0065] The inside of the first cone 102 forms a first cavity 105, and the top of the first cone 102 is provided with an air outlet 106;
[0066] A second cavity 107 is formed inside the cylinder 103, and the aspect ratio of the cylinder 103 is between 3-8;
[0067] The inside of the second cone 104 forms a third cavity 108, the bottom of the...
Embodiment 2
[0087] This example provides a method for cell expansion using the bioreactor described in Example 1.
[0088] Specific steps are as follows:
[0089] (1) Take a Working Cell Bank (WCB) of suspended mammalian cells, thaw and recover in a 37°C water bath, and culture it in a 125mL or 250mL shake flask for 2-5 days. The culture conditions are as follows:
[0090] Temperature: 36-37°C, speed 100-150rpm, CO 2 Concentration 5-7%;
[0091] The selected medium can be selected by those skilled in the art as a commercial medium suitable for the cells.
[0092] (2) Take 20-50 mL of the cell suspension obtained from the culture in step (1) and introduce it into the bioreactor through the feed / outlet 115, and add 5-10 times the volume of the cell suspension as described in step (1) medium, so that the volume of the cell suspension in the bioreactor reaches 150-300mL, cultured at 36-37°C, and fed with 5-7% CO 2 Mixed gas with air, where the ventilation rate is 0.4vvm.
[0093] (3) Aft...
Embodiment 3
[0097] This embodiment provides a method for cell expansion using the bioreactor described in Embodiment 1. Compared with Embodiment 2, the difference lies in:
[0098] The suspension mammalian cells used in step (1) are CHO cells;
[0099] The medium used in step (1) is CD CHO medium.
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