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Breeding method for tissue culture of axillary buds of magnolia sinostellata

A tissue culture and axillary bud technology is applied in the field of plant cultivation of the Magnoliaceae Magnolia, which can solve the problems of inability to meet the needs of garden applications, the seedlings do not have water and humidity resistance, and the rooting rate of cuttings is low, so as to meet the needs of field protection and return, reduce cultivation cost, the effect of ensuring the rooting rate

Pending Publication Date: 2021-03-12
SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the successful tissue culture of Jingning Magnolia. The existing Jingning Magnolia is mainly propagated by cuttings and grafting. Among them, the cutting rooting rate of Jingning Magnolia is low, and the seedlings obtained by grafting do not have its ability to withstand water and humidity. Features, unable to meet the needs of garden applications

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Selection of explants: Jingning magnolia branches with axillary buds were selected in Jingning County, Zhejiang Province in early April, rinsed with tap water for 1 hour, and cut into two sections to obtain branches. into sterile bottles;

[0045] Explant treatment: take out branch section from aseptic bottle, utilize 1% sodium hypochlorite to disinfect after 10min, then rinse 5 times with sterile water, and cut a stem section containing axillary bud from branch section as an explant;

[0046] Anti-browning treatment: Pour the explants into 0.1% PVP, place them in a shaker, keep the temperature in the shaker at 24°C, shake the shaker at 100rpm, shake for 8 hours, and obtain a browning rate of 6.8 % of explants;

[0047] Primary culture: Inoculate the explants after anti-browning treatment into the primary culture medium. The culture conditions are: culture temperature 24°C, light intensity 5000lx, light time 14h / d, culture for 50 days, until adventitious buds are obtai...

Embodiment 2

[0052] Explant selection: In September, the branches of Jingning Magnolia with axillary buds were taken in Xianhu Botanical Garden, Chinese Academy of Sciences, Shenzhen. After removing the leaves, they were washed with tap water for 1 hour, and then the stems with axillary buds were cut off as explants;

[0053] Explant treatment: sterilize the explants with 1% sodium hypochlorite for 12 minutes, wash them with sterile water for 5 times, cut off the excess parts, and keep the axillary buds;

[0054] Anti-browning treatment: Pour the explants treated with explants into 0.1% PVP, and place them in a shaker to keep the temperature in the shaker at 24°C, and shake the shaker at 100rpm for 14 hours to obtain Explants with a browning rate of 8.7%;

[0055] Primary culture: Inoculate the explants after anti-browning treatment into the primary culture medium. The culture conditions are: culture temperature 24°C, light intensity 5000lx, light time 14h / d, and adventitious buds are obta...

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Abstract

The invention is suitable for the field of magnoliaceae magnolia plant cultivation, and discloses a breeding method for tissue culture of axillary buds of magnolia sinostellata. The breeding method comprises the following steps: explant selection, explant treatment, anti-browning treatment, primary culture, subculture, rooting culture and seedling transplantation, wherein a culture medium for primary culture comprises an MS basic culture medium, 0.5-2 mg / L of 6-BA, 0.2-1 mg / L of KT, 0.05-0.2 mg / L of NAA, 0.05-0.1 mg / L of IBA, 7 g / L of agar and 30 g / L of cane sugar, and the pH value of the primary culture medium is 5.8-6.5; a culture medium for subculture comprises the MS basic culture medium, 0.5-1.5 mg / L of 6-BA, 0.05-0.2 mg / L of NAA, 0.05-0.1 mg / L of IBA, 7 g / L of agar and 30 g / L of canesugar, and the pH value of the subculture medium is 5.8-6.5; and a culture medium for rooting culture comprises a 1 / 2MS basic culture medium, 1-2 mg / L of NAA, 1-2 mg / L of IBA, 7 g / L of agar and 30 g / L of cane sugar, and the pH value of the rooting culture medium is 5.8-6.5. According to the breeding method for tissue culture of the axillary buds of the magnolia sinostellata, the propagation speedof the magnolia sinostellata can be increased, and the obtained magnolia sinostellata seedlings can maintain the characteristics of mother seeds.

Description

technical field [0001] The invention belongs to the field of plant cultivation of Magnoliaceae Magnolia, and in particular relates to a breeding method for axillary bud tissue culture of Jingning Magnolia. Background technique [0002] Jingning Mulan Magnolia sinostellata is a deciduous shrub of the genus Magnolia in the family Magnoliaceae. It was published in 1989 and is only distributed in parts of southern Zhejiang. Because of its beautiful tree shape, beautiful flower color and excellent ornamental value, the wild population has been over-harvested. The population has declined sharply in recent years; in addition, due to the abnormal meiosis of the pollen mother cells of Jingning Magnolia, although the species has many flowers, the natural seed setting rate is low. Currently, this species is listed as critically endangered (CR) in the "China Species Red List". [0003] Jingning magnolia often grows beside rivers and streams. It loves water and is resistant to waterlogg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 郎校安李凌飞张苏州杨蕾蕾杨建芬
Owner SHENZHEN XIANHU BOTANICAL GARDEN ADMINISTRATION
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