Method for inhibiting iridovirus proliferation in RAS system micropterus salmoides culture process

A technology of iridescent virus and perch, which is applied in the field of suppressing the proliferation of iridescent virus in the RAS system California perch breeding process, and can solve the problems of fish disease and death

Active Publication Date: 2021-03-12
CHONGQING TECH & BUSINESS UNIV +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention intends to provide a method for inhibiting the multiplication of iridescent virus in the culture process of Calif

Method used

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  • Method for inhibiting iridovirus proliferation in RAS system micropterus salmoides culture process
  • Method for inhibiting iridovirus proliferation in RAS system micropterus salmoides culture process
  • Method for inhibiting iridovirus proliferation in RAS system micropterus salmoides culture process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: the preparation of citrus flavonoids extract

[0028] The general process of extracting citrus flavonoids is: use citrus peels as raw materials (citrus is produced in the Sichuan Basin, and the citrus variety is ponkan), dry until the moisture content is less than 18.0%, crush to a particle size of 0.5-0.9cm, take 10.0g after crushing Powder, the extraction agent is 72%-75% ethanol solution, the ratio of solid to liquid is 1:15-1:20, the ultrasonic power is 310W-350W, the extraction temperature is 38°C-42°C, the extraction time is 48min-58min, after the extraction is completed and filtered to obtain Flavonoid-based extract (citrus flavonoid extract). Dilute to 25mL with 75% ethanol solution, and measure the yield of flavonoids. The extraction yields are shown in Table 1. The extract obtained by the method can better protect the structure of flavonoids, and preserve as many beneficial active ingredients of citrus as possible. In this example, various par...

Embodiment 2

[0035] Embodiment 2: the preparation of black wolfberry anthocyanin extract

[0036] The process of preparing black wolfberry anthocyanin extract is as follows: black wolfberry is used as raw material (black wolfberry is produced in the Qaidam Basin of Qinghai, and the growth place is above 2800 meters above sea level), dried until the moisture content is less than 18.0%, and crushed to a particle size of 0.4 cm-0.6cm, the extractant uses ethanol solution with a volume concentration of 58%-63%, acidifies with 1% hydrochloric acid before use, the ratio of solid to liquid is 1:14-1:17, the ultrasonic power is 280W-320W, and the extraction temperature is 46°C- 50°C, extraction time 30min-40min, the extraction yield of the anthocyanin-based extract (black wolfberry anthocyanin) is shown in Table 2. The extract obtained by this method can better protect the oxidation ability of anthocyanins, and preserve as many beneficial active ingredients in the black wolfberry as possible. In ...

Embodiment 3

[0043] Example 3: An Example of California Bass Culture

[0044] In this example, citrus flavonoid extracts and black wolfberry anthocyanin extracts were added to the RAS system, and California perch was used as the experimental object to verify the efficacy of this breeding method. The addition amount of citrus flavonoid extract and black wolfberry anthocyanin extract was confirmed according to the density of California sea bass, see Table 3 for details. The breeding process is: in the RAS system, according to the dosage and frequency of Table 4, add citrus flavonoids extract and black wolfberry anthocyanin extract (then before adding the two substances to the RAS system, first add the citrus flavonoids extract and black wolfberry The anthocyanin extract was dissolved in warm water at 35°C-40°C to obtain a drug solution, and then the drug solution was added to the RAS system), after a period of continuous feeding, the growth status of California perch was observed, and the ir...

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Abstract

The invention relates to the technical field of aquatic product culture, in particular to a method for inhibiting iridovirus proliferation in a micropterus salmoides culture process of an RAS system.The method comprises the step of adding citrus flavone extract and black Chinese wolfberry anthocyanin extract into the RAS system. According to the method, the citrus flavone extract and the black Chinese wolfberry anthocyanin extract have a synergistic effect, so that the proliferation of iridovirus is inhibited, and the technical problem that the iridovirus in an RAS culture system causes fishto die due to illness is solved. By implementing the scheme, cultured fishes can be prevented from being infected by iridovirus, the survival rate of aquatic products is increased, the quality of theaquatic products is improved, and economic losses are reduced.

Description

technical field [0001] The invention relates to the technical field of aquatic product cultivation, in particular to a method for inhibiting the proliferation of iridescent viruses in the RAS system California perch cultivation process. Background technique [0002] The industrialized recirculating aquaculture (RAS) system adopts an intensive high-density aquaculture model, and uses a series of bioengineering methods to treat aquaculture water so that it can be recycled. All aquaculture water is recycled within the system, and the daily water replenishment coefficient is about 5%. The RAS system has the advantages of low water resource consumption, small land occupation, low environmental pollution, high-quality and safe products, few diseases, high density, breeding production is not restricted and affected by regions or climate, high resource utilization rate, high output and low cost. Risk is an important way to achieve sustainable development of aquaculture. However, d...

Claims

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Application Information

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IPC IPC(8): A61K36/815A61K36/752A61P31/20A61P37/04A01K61/13
CPCA61K36/815A61K36/752A61K36/12A61P31/20A61P37/04A01K61/13A61K2236/33A61K2300/00Y02A40/81
Inventor 周月明唐和礼萨米尔·易卜拉欣·加多申渝王建辉马腾飞
Owner CHONGQING TECH & BUSINESS UNIV
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