Debaryomyces hansenii strain and application in preparation of fruit fresh-keeping agent

A technology of yeast and antistaling agent, which is applied in the application field of preparing fruit antistaling agent, can solve the problems of destroying ecological balance, endangering human health, polluting the environment, etc., and achieves the effects of reducing preservation cost, low cost and prolonging storage time

Active Publication Date: 2021-03-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical agents are still the most widely used to prevent postharvest diseases of fruits, but there are

Method used

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  • Debaryomyces hansenii strain and application in preparation of fruit fresh-keeping agent
  • Debaryomyces hansenii strain and application in preparation of fruit fresh-keeping agent
  • Debaryomyces hansenii strain and application in preparation of fruit fresh-keeping agent

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 Debaryomyces hansenii 'NDB-001'

[0037] The applicant isolated and screened a yeast strain from mangoes in Guangdong. The colonies of this strain on different culture plates are relatively similar in shape, with single cells being oval or round, and the cells are polygonal buds. The colony formed on the plate is oval, milky white, smooth, uniform and viscous in texture, with a high central bulge and umbilicus shape, and the edges of the colony are neat. Ascospores can be formed on the medium, and the cells mate with them to form ascus, and one ascus produces 1-4 spherical ascospores. The above morphological characteristics are similar to those of Debariae spp. described in the literature, and the ITS1 nucleotide sequence of the strain is shown in SEQ ID NO.1; therefore, the strain is named Debariea hansenii 'NDB -001 'Debaryomyceshansenii'NDB-001'. The yeast strain was deposited in the China Center for Type Culture Collection on May 7, 2020. The deposit ...

Embodiment 2

[0040] Embodiment 2: the preparation of fruit fresh-keeping material

[0041] In order to obtain fresh-keeping substances, the present invention shakes and cultures the yeast for 48 hours (YPD liquid medium, culture temperature 20-30° C.), and centrifuges at low temperature (4° C.) to collect the bacteria. Suspend the bacterial pellet with sterile water to make a yeast suspension and adjust the concentration to 1x10 8 cfu / mL is made into fresh-keeping substances.

Embodiment 3

[0042] Embodiment 3: Indoor plate inhibition assay of pathogenic bacteria by fruit fresh-keeping substances

[0043] In order to test the effectiveness of the fresh-keeping material on the growth inhibition of pathogenic bacteria, the present invention takes the fresh-keeping material and places it in half of the two-compartment petri dish. The other half was poured into the culture medium to cultivate pathogenic bacteria, placed in a constant temperature incubator at 25°C, cultivated in the dark, observed and measured the colony diameter with the cross method. Calculate the inhibitory rate of the fresh-keeping material to the pathogenic bacteria by the following formula:

[0044]

[0045] The specific plan is as follows:

[0046] (1) Preparation of test strains: inoculate Botrytis cinerea cultured for about 7 days in the center of one of the two-compartment petri dishes containing PDA medium; pour into the other compartment for cultivating yeast YPD medium.

[0047] (2)...

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Abstract

The invention discloses a debaryomyces hansenii strain and application of the debaryomyces hansenii strain in preparation of a fruit fresh-keeping agent. The strain is classified and named as Debaryomyces hansenii NDB-001, is delivered to China Center for Type Culture Collection (CCTCC) for preservation on May 7th, 2020, and has a preservation number of CCTCC NO: M2020106. According to the invention, the research results find that the Debaryomyces hansenii NDB-001 suspension liquid can be used for effectively inhibiting the growth of botrytis; the storage time of fruits such as apples, juicy peaches, cherries, strawberries, grapes, oranges, mulberries, litchis and the like can be effectively prolonged; and a feasible technical way is provided for fruit preservation, and important significance is provided for maintaining the quality of picked fruits, reducing the storage loss and promoting the sustainable development of the industry.

Description

technical field [0001] The invention belongs to the field of fresh-keeping of agricultural products, and in particular relates to a Debaryomyceshansenii strain Debaryomyceshansenii 'NDB-001' and its application in preparing fruit fresh-keeping agents. Background technique [0002] Fruits are prone to mechanical damage during picking, processing, storage and transportation, and pathogenic bacteria can easily infect fruits through wounds and cause rot. There are about 8×10 3 10,000 tons of fruits cannot be sold due to post-harvest diseases, and the loss is as high as 80 billion yuan. Postharvest diseases of fruits have seriously restricted the development of my country's fruit industry. At present, chemical agents are still the most widely used to prevent and control postharvest diseases of fruits, but there are disadvantages such as high cost, environmental pollution, harm to human health and destruction of ecological balance. With the development of science and the improv...

Claims

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Application Information

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IPC IPC(8): C12N1/16A23B7/155C12R1/645
CPCA23B7/155A23V2002/00A23V2200/10A23V2250/76
Inventor 窦道龙田月娥叶琴沈丹宇徐衡胡白石
Owner NANJING AGRICULTURAL UNIVERSITY
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