Novel application of L-fucose
A technology of fucose, action, applied in the field of medicine
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] Example 1 Effect of L-fucose on the extension degree of hair follicles and hair shafts cultured in vitro
[0040] Hair follicles were obtained from patients undergoing hair transplant surgery at the Department of Dermatology, Huashan Hospital Affiliated to Fudan University, with the consent of the patients.
[0041] Hair follicles were isolated and incubated: hair follicles in the anagen phase were picked out, one-third cut off from the upper part, and the remaining two-thirds were placed in Williams E medium (Sigma). The concentration of L-fucose or D-fucose added to the culture medium was 200 μg / ml. The normal group was placed in the culture medium without any treatment. All groups were placed in a 37°C, 5% CO2 incubator (Thermo 371) for 3 days and 6 days, and the medium was replaced every 2 days. On days 3 and 6, in vitro hair follicles in culture were photographed, and hair shaft length was measured and recorded. see results figure 1 .
[0042] Depend on figure...
Embodiment 2
[0043] Example 2: Effect of L-fucose on growth of dermal papilla cells
[0044] The dermal papilla was dissected from the bulbous section using an operating microscope and then transferred to a Petri dish containing DMEM medium (Sigma). The medium contains 50-100 U / ml penicillin, 50-100 μg / ml streptomycin and 20% heat-inactivated fetal calf serum (Hyclone). Dermal papilla cells were obtained by directly digesting the dermal sheath tissue at the lower part of the hair follicle by collagenase D digestion. Put the culture dish into a 7°C, 5% CO2 incubator, change the medium every 2 days, and culture for 3-4 days until the confluency reaches 90%, and then passage the cells. Take out the cells from the incubator, observe the cell morphology with an inverted microscope and record it. Use a pipette to suck out the medium in the culture dish and wash it twice with DPBS. Add 0.25% Tripsin-EDTA for digestion, place the cells in a 37°C incubator, until 80-90% of the cells are observed...
Embodiment 3
[0047] Example 3 Effect of L-fucose on gene expression levels of β-catenin, Bcl-2, Bax, MMP-2, MMP-9, VEGF, IGF-1, KGF-2
[0048] 1. Test method: The dermal papilla cells were cultured in a medium containing 100 μg / ml of L-fucose or D-fucose. The normal group only added the medium, and the medium in the model group was added at a concentration of 30 μg / ml of testosterone, incubated for 3 days, then extracted RNA for reverse transcription and Real-time PCR analysis.
[0049] Table 1 Reagents (boxes) used for RNA extraction, reverse transcription PCR, and Real-time PCR
[0050]
[0051] Table 2 The main instruments used in RNA extraction, reverse transcription, and Real-time PCR
[0052]
[0053] Avoid RNA degradation during RNA extraction, operate on ice, and try to move as quickly as possible. In the experiment, RNeasy Mini Kit was used to extract total RNA. cDNA was obtained by reverse transcription reaction using Rever Tra Ace-α Transcriptase Kit. Choose to use th...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com