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Novel application of L-fucose

A technology of fucose, action, applied in the field of medicine

Pending Publication Date: 2021-03-26
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the prior art, there are no related reports about L-fucose in hair loss prevention and hair growth

Method used

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  • Novel application of L-fucose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Effect of L-fucose on the extension degree of hair follicles and hair shafts cultured in vitro

[0040] Hair follicles were obtained from patients undergoing hair transplant surgery at the Department of Dermatology, Huashan Hospital Affiliated to Fudan University, with the consent of the patients.

[0041] Hair follicles were isolated and incubated: hair follicles in the anagen phase were picked out, one-third cut off from the upper part, and the remaining two-thirds were placed in Williams E medium (Sigma). The concentration of L-fucose or D-fucose added to the culture medium was 200 μg / ml. The normal group was placed in the culture medium without any treatment. All groups were placed in a 37°C, 5% CO2 incubator (Thermo 371) for 3 days and 6 days, and the medium was replaced every 2 days. On days 3 and 6, in vitro hair follicles in culture were photographed, and hair shaft length was measured and recorded. see results figure 1 .

[0042] Depend on figure...

Embodiment 2

[0043] Example 2: Effect of L-fucose on growth of dermal papilla cells

[0044] The dermal papilla was dissected from the bulbous section using an operating microscope and then transferred to a Petri dish containing DMEM medium (Sigma). The medium contains 50-100 U / ml penicillin, 50-100 μg / ml streptomycin and 20% heat-inactivated fetal calf serum (Hyclone). Dermal papilla cells were obtained by directly digesting the dermal sheath tissue at the lower part of the hair follicle by collagenase D digestion. Put the culture dish into a 7°C, 5% CO2 incubator, change the medium every 2 days, and culture for 3-4 days until the confluency reaches 90%, and then passage the cells. Take out the cells from the incubator, observe the cell morphology with an inverted microscope and record it. Use a pipette to suck out the medium in the culture dish and wash it twice with DPBS. Add 0.25% Tripsin-EDTA for digestion, place the cells in a 37°C incubator, until 80-90% of the cells are observed...

Embodiment 3

[0047] Example 3 Effect of L-fucose on gene expression levels of β-catenin, Bcl-2, Bax, MMP-2, MMP-9, VEGF, IGF-1, KGF-2

[0048] 1. Test method: The dermal papilla cells were cultured in a medium containing 100 μg / ml of L-fucose or D-fucose. The normal group only added the medium, and the medium in the model group was added at a concentration of 30 μg / ml of testosterone, incubated for 3 days, then extracted RNA for reverse transcription and Real-time PCR analysis.

[0049] Table 1 Reagents (boxes) used for RNA extraction, reverse transcription PCR, and Real-time PCR

[0050]

[0051] Table 2 The main instruments used in RNA extraction, reverse transcription, and Real-time PCR

[0052]

[0053] Avoid RNA degradation during RNA extraction, operate on ice, and try to move as quickly as possible. In the experiment, RNeasy Mini Kit was used to extract total RNA. cDNA was obtained by reverse transcription reaction using Rever Tra Ace-α Transcriptase Kit. Choose to use th...

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Abstract

The invention relates to the field of medicines, and relates to a novel application of L-fucose, in particular to an application of L-fucose in preparation of products with hair loss preventing and hair growing effects. In-vitro experiments find that L-fucose can significantly up-regulate expression (p is smaller than 0.001) of a beta-catenin gene, an IGF-1 gene, a KGF-2 gene and VEGF gene and promote expression (p is smaller than 0.001) of an MMP-2 gene and an MMP-9 gene, and is helpful for inducing or maintaining hair follicle growth period and promoting hair follicle growth and dermal papilla extracellular matrix remodeling; and meanwhile, the expression (p is smaller than 0.001) of the anti-apoptosis gene bcl-2 is remarkably up-regulated, and the ratio (p is smaller than 0.005) of Bax / Bcl-2 is down-regulated, so that the apoptosis of dermal papilla cells is inhibited. The result shows that the L-fucose has good hair loss preventing and hair growing effects.

Description

technical field [0001] The invention relates to the field of medicines, in particular to the new application of L-fucose, in particular to the application of L-fucose or its polymer in the preparation of products with hair loss prevention and hair growth functions. Background technique [0002] Hair is a natural polymer keratin fiber, which can be divided into four parts: dermal papilla, hair follicle, hair root and hair shaft from bottom to top. It is chemically inactive, but sensitive to light, boiling water, acids, alkalis, oxidizing and reducing agents. Frequent perm dyeing, improper stretching and bad weather, staying up late, irregular diet, and excessive mental stress in daily life will cause damage to hair and even fall out. The physiological characteristics and functions of hair mainly depend on the dermal papilla, hair follicles and sebaceous glands under the scalp surface. [0003] Hair follicles are constantly replaced in a cycle of various stages of proliferat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61K8/73A61Q7/00A23L33/125A23L29/30A23P10/30A23P10/28A23P10/40A23P10/20A23L2/38A23L2/60A23F3/14A23F3/34
CPCA61K8/60A61K8/73A61Q7/00A23L33/125A23L29/30A23P10/30A23P10/28A23P10/40A23P10/20A23L2/38A23L2/60A23F3/14A23F3/34A23V2002/00A23V2200/318A23V2250/60
Inventor 朱华伟李泓颖罗珍余庆涛唐健
Owner INFINITUS (CHINA) CO LTD
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