A Fast Single Particle Identification Method Based on Femtosecond Laser Modulation Phase
A phase modulation, femtosecond laser technology, applied in the field of optical imaging, can solve the problems of inability to achieve living body detection, in-situ non-destructive testing, etc.
Active Publication Date: 2022-05-31
SHANXI UNIV
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These methods have very high spatial resolution, but the sample needs to be specially prepared, which cannot realize in situ non-destructive testing, especially in vivo detection
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[0043] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0044] like figure 1 As shown, a single particle rapid identification device based on femtosecond laser modulation phase in this embodiment includes a femtosecond laser double beam preparation system 101, a laser self-interference light intensity detection system 102, and a multiphoton fluorescence excitation and collection system. 103. Four parts of the data extraction and processing system 104;
[0045] The femtosecond laser dual beam preparation system 101 includes a femtosecond laser 105, a polarizer 106, a mirror 107, an equal-ratio beam mirror 108, a first corner cube mirror 110, and a second corner cube mirror 112 , an electro-optical modulation crystal 113 and an electro-optical modulation crystal controller 114;
[0046] The femtosecond laser 105 is used to excite metal nanoparticles to generate multiphoton fluorescence, and the center waveleng...
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Abstract
The invention relates to a device and method for fast identification of single particles based on femtosecond laser modulation phase, the purpose is to overcome the limitation that traditional optical imaging cannot identify single metal nanoparticles, and use femtosecond laser to excite and modulate the multiphoton fluorescence process of metal nanoparticles , by comparing the femtosecond laser self-interference phase with the multiphoton fluorescence modulation phase of metal nanoparticles, the identification of individual metal nanoparticles is realized. The present invention consists of four parts: a femtosecond laser double-beam preparation system, a laser self-interference light intensity detection system, a multiphoton fluorescence excitation and collection system, and a data extraction and processing system; compared with traditional dark-field imaging and fluorescence imaging, no Breaking through the diffraction limit, individual metal nanoparticles can be identified. Compared with characterization methods such as scanning electron microscopy and atomic force microscopy, it does not require special preparation of samples, and does not require measurement under vacuum conditions. In-situ non-destructive detection can be achieved, and the detected samples can continue to be used for other measurements or applications.
Description
technical field [0001] The invention belongs to the technical field of optical imaging, and in particular relates to a single particle rapid identification method based on femtosecond laser modulation phase. Background technique [0002] Precious metal nanoparticles, such as gold and silver nanoparticles, have localized surface plasmon resonance (LSPR). LSPR can localize incident electromagnetic waves in the nanoscale range, significantly enhance the intensity of electromagnetic waves, and realize the amplification and modulation of optical signals. At the same time, metal nanoparticles have good luminescence properties, photostability and biocompatibility, so metal nanoparticles have important applications in biomedicine, chemical reaction tracking, analytical sensing and so on. [0003] The size of metal nanoparticles is usually below 100 nm. Identifying the number and aggregation state of metal nanoparticles, such as single particles, dimers, multimers, etc., is the basi...
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IPC IPC(8): G01N21/64G01J1/00G01N21/01
CPCG01N21/6402G01J1/00G01N21/01Y02A90/10
Inventor 秦成兵李耀陈瑞云韩双萍张国峰胡建勇景明勇肖连团贾锁堂
Owner SHANXI UNIV