Application method of fluorescence correlation spectroscopy (FCS) in plant cell nucleus

Abstract

The invention discloses an application method of fluorescence correlation spectrum (FCS) in plant cell nucleus. The application method comprises the steps of 1, fluorescence modulation; 2, plant processing; 3, microscopic imaging; 4, cell analysis; and 5, cleaning treatment. In the step 1, proper fluorophore raw materials are manually selected according to plant cells; and the selected fluorophore raw materials are mixed with water, and the mixture is subjected to oscillation, standing and storage. According to the application method of the fluorescence correlation spectrum (FCS) in the plant cell nucleus, the plant cell nucleus emits fluorescence by adopting the fluorophore raw materials, and the fluorescence correlation spectrum is drawn by adopting an electron microscope, so that convenience and rapidness are achieved, observation of the plant cell nucleus, description of plant cell nucleus characteristics and analysis of the physiological and biochemical process of the plant cell nucleus are facilitated, and the process is simple and rigorous, can replace other expensive fluorescence correlation spectrum technologies, has the advantages of obvious effect, cheap materials and low cost, greatly saves the cost, and is beneficial to the use of users.

Description

technical field [0001] The invention relates to the technical field of plant cell nuclei, in particular to an application method of fluorescence correlation spectroscopy (FCS) in plant cell nuclei. Background technique [0002] The plant cell is the basic unit of the structure and function of plant life activities. It is composed of protoplast and cell wall. Protoplast is the general term for all substances in the cell wall. It is mainly composed of cytoplasm and nucleus. Organelles, in addition to cell fluid and subsequent inclusions, etc., generally use fluorescence correlation spectroscopy to analyze mechanical energy. The traditional application of fluorescence correlation spectroscopy in plant cell nuclei is complicated and costly. Fluorescent raw materials are used directly to make plant cell nuclei emit fluorescence. The fluorescence correlation spectrum cannot be drawn, which is not conducive to the observation of plant cell nuclei, the characteristics of plant cell ...

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Problems solved by technology

[0002] The plant cell is the basic unit of the structure and function of plant life activities. It is composed of protoplast and cell wall. Protoplast is the general term for all substances in the cell wall. It is mainly composed of cytoplasm and nucleus. Organelles, in addition to cell fluid and subsequent inclusions, etc., generally use fluorescence correlation spectroscopy to analyze mechanical energy. The traditional application of fluorescence correlation spectroscopy in plant cell nuclei is complex and costly. Fluorescent raw materials are directly used to make plant cell nuclei emit fluorescence. The fluorescence correlation spectrum cannot be drawn, which is not conducive to the observation of plant cell nuclei, the characteristics of plant cell nuclei cannot be described, and the physiological and biochemical processes of plant cell nuclei cannot be analyzed; in view of these defects, it is necessary to design the application method of fluorescence correlation spectroscopy (FCS) in plant cell nuclei

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