Tissue engineering patch and preparation method thereof

Abstract

The invention relates to a tissue engineering patch and a preparation method thereof. The preparation method comprises the following steps: (1) inoculating target cells on one side of the decellularized scaffold, placing pyroptotic products on the other side of the decellularized scaffold, The pyroptotic product is separated from the surface of the other side of the decellularized scaffold; then the decellularized scaffold inoculated with the target cells and the pyroptotic product are jointly cultivated in the culture medium; (2) when the target cell When migrating from one side of the decellularized scaffold to the other side, the non-migrated target cells on the surface of the decellularized scaffold are removed to obtain a tissue engineering patch; the pyroptosis product is produced after cell pyroptosis At least one of pyroptotic secretions, pyroptotic extracts and pyroptotic cells. The tissue engineering patch prepared by the method of the invention has a high cell loading capacity, and the target cells are uniformly distributed in the scaffold and have good biological activity.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a tissue engineering patch and a preparation method thereof. Background technique [0002] Stem cell therapy has achieved good therapeutic effects both experimentally and clinically, showing bright application prospects. At present, the methods of using stem cells or delivering stem cells in the field of stem cell therapy are mainly divided into three categories: 1. Direct injection of stem cells; 2. Stem cell sheet technology; 3. Tissue engineering patch technology. These three types of treatment methods have their own advantages and disadvantages. The use of stem cell injection is simple and easy to repeat, causing little damage to patients, but the effective use rate of stem cells is low, especially when intravenous injection is used as the delivery method of stem cells, most of the stem cells cannot be in the damaged tissue. Play a therapeutic role, and due to the inflammat...

AI Technical Summary

Problems solved by technology

The advantage of this method is that the use of gel to encapsulate the seed cells provides a three-dimensional void structure for cell growth and proliferation while providing protection for the cells, but the disadvantage is also obvious that the gel can only be attached to the surface of the scaffold material, and cannot be evenly distributed. throughout the scaffold material, and the gel forms a resistance for cell migration into the interior of the scaffold

The dynamic culture system method is to immerse the scaffold material in a high-density cell suspension, and use rotation, centrifugation, and shaking culture operations to inoculate the cells on the scaffold material. Compared with the immersion method and precipitation method, the dynamic culture system can make the cells more uniform. And more adsorbed on the scaffold material and more conducive to the migration of cells to the interior of the scaffold material, but at the same time as the physical factors imposed by the dynamic culture system, rotation, centrifugation and shaking will also cause loosely adhered cells to fall off the scaffold, Or cause damage to the cells, affecting the inoculation rate of the seed cells and the activity of the seed cells

Although the above cell construction techniques can achieve the purpose of compounding seed cells and scaffold materials, the cell load of the scaffold material after inoculation is low, the cells are unevenly distributed in the scaffold material, and how to ensure the quality of seed cells during the inoculation process is still a problem. issues that need resolving

[0004] Although tissue engineering patch technology has a wide range of application scenarios and bright prospects, the insufficiency of existing construction technologies limits the application and therapeutic effect of tissue engineering patch

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