Blood fat adsorbent and preparation method thereof

A technology of adsorbent and blood lipid, which is applied in the field of blood lipid adsorbent and its preparation, can solve the problems of low reaction efficiency, inability to obtain blood lipid adsorbent, and low utilization rate of ligand, so as to improve adsorption efficiency, improve catalytic ring-opening and coupling The effect of improving the efficiency of the coupling reaction and the efficiency of the coupling reaction

Active Publication Date: 2021-03-30
WUHAN RUIFA MEDICAL DEVICES CO LTD
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  • Abstract
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Problems solved by technology

However, in the actual coupling reaction, the utilization rate of the ligand dextran sulfate is less than 1%, and the effective blood lipid adsorbent cannot be obtained when the reacted ligand solution is reused. It can be seen that the reaction efficiency of the coupling reaction Low and low ligand utilization
[0005] In view of this, it is necessary to design an improved method for the preparation of lipid adsorbents to solve the problems of low reaction efficiency and low utilization of ligands during the preparation of adsorbents.

Method used

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preparation example Construction

[0026] The invention provides a preparation method of a blood lipid adsorbent, comprising the following steps:

[0027] S1. Activating the adsorption carrier to obtain an epoxy-containing activation carrier;

[0028] The epoxy-containing activation carrier includes, but is not limited to, one or more of epoxy-containing sepharose, cellulose gel and resin, preferably sepharose microspheres. For example, epichlorohydrin is used to activate the agarose gel microspheres to obtain epoxy group-containing agarose gel microspheres.

[0029] S2. adding the lipid adsorption ligand into the buffer to obtain a ligand solution;

[0030] The blood lipid adsorption ligand is a dextran sulfate solution or a heparin solution; the buffer is a glycine-sodium hydroxide buffer.

[0031] S3. The epoxy-containing activation carrier obtained in step S1 is mixed with a tertiary amine reagent, and then added to the ligand solution obtained in step S2, and the coupling reaction is carried out with mix...

Embodiment 1

[0040] A preparation method of a blood lipid adsorbent, comprising the following steps:

[0041] S1. Take a certain amount of agarose gel microspheres, add 1.0mol / L sodium hydroxide solution and epichlorohydrin in a ratio of 1g:1.5ml:450μl to the microspheres, mix well, and react at 40°C for 2 hours , washed and drained to obtain activated agarose gel microspheres;

[0042] S2. Dissolve dextran sulfate sodium salt in a glycine-sodium hydroxide buffer with a pH value of 10.6 at a ratio of 200mg: 1ml to prepare a dextran sulfate ligand solution;

[0043] S3. Mix the activated sepharose microspheres with dodecyl tertiary amine by 1g:0.6ml, and then press the ratio of activated sepharose microspheres to dextran sulfate ligand solution to be 1g:2.5ml , adding it into the dextran sulfate ligand solution, carrying out a coupling reaction at 50° C. for 8 hours, and then washing the adsorption carrier with purified water, brine, ethanol and purified water in turn to obtain the blood l...

Embodiment 2

[0045] A preparation method of a blood lipid adsorbent, compared with Example 1, is different in that the dodecyl tertiary amine in step S3 is replaced with hexadecyl tertiary amine. Others are basically the same as those in Embodiment 1, and are not repeated here.

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Abstract

The invention provides a blood fat adsorbent and a preparation method thereof. The method comprises the following steps: firstly, carrying out activation reaction on an adsorption carrier to obtain anepoxy group-containing activated carrier; then adding a blood fat adsorption ligand into a glycine-sodium hydroxide buffer solution to obtain a ligand solution; mixing the obtained epoxy group-containing activated carrier with a tertiary amine reagent, then adding the mixture into a ligand solution, mixing and stirring to carry out a coupling reaction, and after the reaction is completed, carrying out solid-liquid separation, cleaning and drying to obtain the blood fat adsorbent. According to the invention, the tertiary amine reagent is added into the coupling reaction system, the tertiary amine reagent is firstly adsorbed on the surface of the epoxy group-containing activated carrier, and then the coupling reaction is carried out in the glycine-sodium hydroxide buffer solution ligand solution, so that the coupling reaction efficiency and the ligand utilization rate can be improved, the adsorption efficiency of the adsorbent is improved, and the production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of preparation of medical materials, in particular to a blood lipid adsorbent and a preparation method thereof. Background technique [0002] Hyperlipidemia is a condition in which one or more lipids in the plasma are higher than normal due to abnormal fat metabolism or operation Lipoprotein is too low, which is called "dyslipidemia" in modern medicine. Hyperlipidemia is a common and frequently-occurring disease, and it is also the culprit of cardiovascular and cerebrovascular diseases. The damage to the body is hidden, gradual, progressive and systemic. Its direct damage is to accelerate systemic atherosclerosis. . Among them, low-density lipoprotein is a lipoprotein particle that carries cholesterol into peripheral tissue cells. When low-density lipoprotein is excessive, the cholesterol it carries will accumulate on the arterial wall, which is easy to cause atherosclerosis for a long time. Therefore, LD...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/24B01J20/30
CPCB01J20/24B01J20/22
Inventor 张媛张磊陈梦林沈乔皮云王业富
Owner WUHAN RUIFA MEDICAL DEVICES CO LTD
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