Composition for inhibiting aeromonas as well as preparation method and application thereof
A technology of composition and single-cell bacteria, which is applied in the fields of application, antibacterial drugs, animal feed, etc., can solve the problems of emerging drug-resistant strains in an endless stream
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Embodiment 1
[0032] The preparation of embodiment 1 Caulis Spatholobus extract
[0033] Millet Spatholobus was crushed and sieved to obtain Spatholobus Spatholobus powder, and 600g of powder was accurately weighed and heated at 80°C for 20 minutes; Spatholobus Spatholobus powder was added to 20 times the concentration of 50% ethanol, extracted for 12 hours to obtain extract A, and the second part of Spatholobus Spatholobus powder was added to 20 times the concentration of 60% ethanol, Leach for 12 hours to obtain extract solution B, add the third part of Caulis Spatholobus powder to 20 times the concentration of 90% ethanol, extract for 48 hours to obtain extract solution C; The liquid A, the extract B and the extract C are uniformly mixed and then filtered, and the obtained filtrate is rotary evaporated to obtain the extract A of Spatholobus Spatholobus.
[0034] Millet Spatholobus was crushed and sieved to obtain Spatholobus Spatholobus powder, and 600g of powder was accurately weighed ...
Embodiment 2
[0043] Example 2 The bacteriostasis effect of Caulis Spatholobus extract on Aeromonas hydrophila
[0044] 1. Preparation of culture medium
[0045] BHI medium was purchased from BD Company in the United States; 5% sheep blood agar plate, bacterial biochemical trace identification tube, etc. were purchased from Guangzhou Huankai Microbiology Co., Ltd.
[0046] 2. Preparation of bacterial suspension
[0047] Test bacteria culture: Activate the Aeromonas hydrophila strain, streak on the solid medium with an inoculation needle, and culture at 30°C for 24 hours, pick a single colony, inoculate it on the nutrient broth medium and shake it for 24 hours. The bacterial solution was diluted to an appropriate concentration by plate counting method, and the concentration of the bacterial solution was 1×108 CFU / mL.
[0048] 3. Drug sensitivity test
[0049]The susceptibility test adopts the agar diffusion method, dilutes the bacterial suspension cultured to the logarithmic growth phase ...
Embodiment 3
[0056] Embodiment 3 The influence of the proportioning of the extract of Spatholobus Spatholobus and the extract of Spathiphyllum chinensis on the antibacterial effect
[0057] Millet Spatholobus was crushed and sieved to obtain Spatholobus Spatholobus powder, and 600g of powder was accurately weighed and heated at 80°C for 20 minutes; Spatholobus Spatholobus powder was added to 20 times the concentration of 50% ethanol, extracted for 12 hours to obtain extract A, and the second part of Spatholobus Spatholobus powder was added to 20 times the concentration of 60% ethanol, Leach for 12 hours to obtain extract solution B, add the third part of Caulis Spatholobus powder to 20 times the concentration of 90% ethanol, extract for 48 hours to obtain extract solution C; The solution A, the extract solution B and the extract solution C are uniformly mixed and then filtered, and the obtained filtrate is rotary steamed to obtain the extract of Spatholobus Spatholobus.
[0058] Grind and...
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