A spermatogonia separation liquid and its application in the separation and purification of spermatogonia

A technology of Channa japonica and spermatogonia, which is applied in the field of separation of fish germ cells, can solve the problems of unsatisfactory separation and purification effect and high price, and achieve the effect of good vitality, good proliferation and differentiation ability
CN112608887BActive Publication Date: 2021-12-07SUN YAT SEN UNIV
4 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2021-12-07

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention belongs to the technical field of separation of fish germ cells, and in particular relates to a spermatogonia separation liquid and its application in separating and purifying the spermatogonia of S. The separation and purification method of protocells, the present invention provides a spermatogonia separation liquid, the separation liquid includes testis digestion solution and percoll gradient solution, and the testis digestion solution includes L-15 medium, type IV collagenase, DNase I , trypsin, EDTA, the percoll gradient solution is obtained by diluting the 100% percoll stock solution with perocll diluent in different multiples, and the separation solution is used to separate and purify the spermatogonia of Channa mongolica, which can obtain high purity and high activity The spermatogonial cells of Channa chinensis can proliferate and differentiate in vitro.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of separation of fish germ cells, and in particular relates to a spermatogonia separation liquid and its application in separating and purifying the spermatogonia of Channa japonica. Background technique

[0002] Fish is an important biological resource. In recent years, due to habitat degradation and overfishing, fishery resources in natural waters have declined sharply, resulting in nearly 3,000 species of fish being endangered to varying degrees. Therefore, how to carry out long-term and effective protection of fish germplasm resources has become an urgent problem to be solved.

[0003] Germ cells are the only cells that can transmit genetic material to the next generation, and are important germplasm resources and the basis for population reproduction. In the early stage of fish embryonic development, germ cells specialize to form primordial germ cells (primordial germ cells, PGCs). Subsequently, PGCs...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More