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Aspergillus flavus RPA primer, kit, aspergillus flavus detection method and aspergillus flavus detection device

A kind of Aspergillus flavus detection method technology, which is applied in the field of detection method and device of Aspergillus flavus, kit, RPA primer of Aspergillus flavus. The effect of protecting food quality, simplifying requirements, and reducing pollution

Active Publication Date: 2021-04-06
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Still another object of the present invention is to provide a kind of RPA primer of Aspergillus flavus, test kit, and detection method and device of Aspergillus flavus, solve the problem of existing food The biological detection method of Aspergillus flavus in China is cumbersome, time-consuming, low-sensitivity and unable to be operated on-site

Method used

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  • Aspergillus flavus RPA primer, kit, aspergillus flavus detection method and aspergillus flavus detection device
  • Aspergillus flavus RPA primer, kit, aspergillus flavus detection method and aspergillus flavus detection device
  • Aspergillus flavus RPA primer, kit, aspergillus flavus detection method and aspergillus flavus detection device

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example 1

[0047] 1. On-site rapid extraction of DNA from corn samples

[0048] 1) Representative sampling is carried out according to the three-layer five-point method: according to the size of the sample area, it is divided into several squares, and each block is a district. Each district is divided into three layers according to upper, middle and lower, and each layer has five points in the center and four corners. According to the area and point, take a small amount of samples with the sampler first up and down, and then process the samples according to the quartering method.

[0049] 2) Extract DNA from the corn sample obtained above: Aseptically weigh 25g of the sample to be tested and place it in a 50mL disposable syringe. After absorbing sterile water, shake vigorously for 3 minutes, drain the water, and absorb clean sterile water again , discharged after shaking for 30s, and repeated 2 times to wash off the spores and hyphae of Aspergillus flavus that may be contaminated on the...

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Abstract

The invention discloses an aspergillus flavus RPA primer, a kit, an aspergillus flavus detection method and an aspergillus flavus detection device. The aspergillus flavus RPA primer and the aspergillus flavus detection kit are high in specificity, high in sensitivity and accurate in detection result. The invention also provides an aspergillus flavus rapid detection method and an aspergillus flavus rapid detection device in food, the detection method comprises the following steps: performing on-site rapid extraction of DNA of a sample, performing RPA rapid amplification by using an RPA primer with the DNA as a template to obtain a DNA amplification product, and judging whether aspergillus flavus pollution exists in the sample or not according to the fluorescence phenomenon of the DNA amplification product. The aspergillus flavus detection device comprises a self-made portable suction filter and a simple mortar. The DNA extraction method is rapid and efficient, RPA rapid amplification can be carried out at the body temperature, aspergillus flavus can be accurately, rapidly and efficiently detected within 30 min under the 37 DEG C isothermal condition, meanwhile, the requirement for an experiment place is simple, complex instruments are not needed, and the DNA extraction method is quite suitable for sampling inspection of basic samples.

Description

technical field [0001] The invention relates to the technical field of food safety detection. More specifically, the present invention relates to an RPA primer of Aspergillus flavus, a kit, and a detection method and device for Aspergillus flavus. Background technique [0002] Aspergillus flavus is a type of fungus that lives in a parasitic or saprophytic manner. It is characterized by relatively developed mycelium and no large fruiting bodies. Aspergillus flavus can produce spores for reproduction and transmission, and it reproduces rapidly in warm and humid environments. Aspergillus flavus can infect crops in every link of planting, harvesting, storage, transportation and processing, making food mildew and losing its edible value, resulting in huge waste and economic losses. At the same time, it can produce toxic secondary metabolites - aflatoxin during its growth. Aspergillus flavus and the aflatoxin produced by it have brought a huge threat to food safety and human h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6844C12Q1/04C12N15/11C12M1/34C12M1/12C12M1/00
CPCC12Q1/6895C12Q1/6844C12Q2531/119C12Q2563/107
Inventor 邢福国刘启君靳婧李旭
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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