Surface plasma coupling emission directional enhancement type microscopic fluorescence imaging and spectrum detection method and device based on objective lens coupling type

A surface plasmon and microfluorescence technology, which is applied in the field of microscopic optical imaging and spectral analysis, can solve the problems of large detection range, inability to detect samples, and inability to obtain sample spectral information, so as to improve signal detection sensitivity and improve signal collection. Efficiency and detection sensitivity, the effect of reliable detection means

Active Publication Date: 2021-04-13
XIAMEN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, although the SPCE imaging and spectral detection device based on the prism configuration can obtain the SPCE fluorescence imaging and spectral information of the sample at the same time, its detection range is large, and it cannot detect samples with a micro-area size, which limits the application of SPCE

Method used

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  • Surface plasma coupling emission directional enhancement type microscopic fluorescence imaging and spectrum detection method and device based on objective lens coupling type
  • Surface plasma coupling emission directional enhancement type microscopic fluorescence imaging and spectrum detection method and device based on objective lens coupling type
  • Surface plasma coupling emission directional enhancement type microscopic fluorescence imaging and spectrum detection method and device based on objective lens coupling type

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Embodiment

[0049] Please check figure 1 and figure 2 , is a kind of SPCE directional enhanced micro-fluorescence imaging and spectral detection device based on the objective lens coupling type of this embodiment, which includes: excitation light module, directional excitation SPCE microscope module, SPCE micro-fluorescence imaging module, SPCE micro-fluorescence Spectrum module.

[0050] The excitation light module includes a laser 1 and a fiber coupler 2 connected to each other; the excitation beam emitted by the excitation light module travels along the first optical path direction;

[0051] The directional excitation SPCE microscope module includes a collimating lens 3, a half-wave plate 4, a first focusing lens 5, a sample 6, an objective lens 7, a dichroic filter 8, a second focusing lens 9, and a 90° adjustable total reflection mirror 10; the half-wave plate 4 can adjust the polarization of the excitation light to p polarization, such as image 3 The sample 6 is placed on the s...

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Abstract

The invention discloses a surface plasma coupled emission (SPCE) directional enhanced microscopic fluorescence imaging and spectrum detection method and device based on an objective lens coupling type. Polarized exciting light modulated by a half-wave plate is reflected by a dichroic filter and then reaches an objective lens at a specific angle to excite metal surface plasma to be coupled to a sample, and generated SPCE signals are collected by the objective lens and filtered by the dichroic filter and then are switched and guided into an SPCE microscopy fluorescence imaging module or an SPCE microscopy fluorescence spectrum module through a reflector to realize SPCE microscopic fluorescence imaging and spectrum acquisition of the same micro-area of the sample. By combining a microscope system, a coupling strategy SPCE is applied to high-spatial-resolution fluorescence imaging and spectrum at the same time, the directional enhancement characteristic and the spatial selection function on the micro-area scale are achieved, high-quality microscopic fluorescence imaging and spectrum information of the same micro-area of a sample can be obtained, and an efficient and reliable means is provided for detecting the low-content components of a micro area of interest in a complex system.

Description

technical field [0001] The invention relates to the technical field of microscopic optical imaging and spectral analysis, in particular to a method and device for directional enhanced microscopic fluorescence imaging and spectral detection based on surface plasmon coupled emission (SPCE). Background technique [0002] With the continuous development of cells and small molecules in the fields of biology and medicine, it also puts forward higher requirements for microscopic analysis. The microscopic imaging and spectroscopy device is a fusion of optical imaging technology and spectroscopy technology. It can not only provide spatial imaging information of the target object, but also provide rich spectral information, providing a new technical means for studying or observing tiny objects. Fluorescence technology has the advantages of fast imaging speed, good repeatability, and intuitive observation, and has been more and more widely used in the fields of cells and small molecule...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6402
Inventor 李耀群潘晓会徐子谦陈敏曹烁晖
Owner XIAMEN UNIV
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