Acipenser schrenckii sperm cryopreservation solution and preservation method

A technology of cryopreservation solution and preservation method, which is applied in the field of stylized sturgeon breeding to achieve the effects of improving efficiency, improving sperm motility, and increasing cleavage rate

Inactive Publication Date: 2021-04-16
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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However, the sperm preservation and sperm quality of

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  • Acipenser schrenckii sperm cryopreservation solution and preservation method

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Embodiment 1

[0028] A kind of cryopreservation liquid of sturgeon sperm, this cryopreservation liquid is made by comprising the following components by weight: 1.8 parts of sodium chloride, 2.6 parts of potassium chloride, 8.2 parts of ethylene glycol, 3.5 parts of sodium dihydrogen phosphate, 0.54 parts of pyridoxine, 0.26 parts of GSH, 0.05 parts of HA, 0.13 parts of SDS, 0.74 parts of Vc.

[0029] The preparation method of the sperm cryopreservation solution of Stryn's sturgeon comprises the following steps: dissolving each component in distilled water of 5 times the amount, and mixing evenly.

[0030] A method for preserving the sperm of Acipenser's sturgeon, comprising the following steps: lowering the temperature of the cryopreservation solution to 5-6°C, then mixing and shaking the semen of Acipenser's sturgeon with 5 times the amount of the cryopreservation solution, Then divide the mixed solution, pre-cool the small-packed mixed solution at 1-3°C for 30 minutes, then place the pre...

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Abstract

The invention provides an acipenser schrenckii sperm cryopreservation solution and a preservation method. The cryopreservation solution is prepared from the following components in parts by weight: 1-5 parts of sodium chloride, 1-5 parts of potassium chloride, 5-10 parts of ethylene glycol, 3-10 parts of sodium dihydrogen phosphate, 0.5-2 part of pyridoxine, 0.1-1 part of GSH, 0.01-1 part of HA, 0.1-1 part of SDS and 0.5-2 part of Vc. According to the acipenser schrenckii sperm cryopreservation liquid, damage of freezing to sperm cell structures and genetic materials is reduced, and the survival rate and fertilization rate of frozen sperms are increased.

Description

technical field [0001] The invention belongs to the field of sturgeon breeding, and in particular relates to a sperm cryopreservation solution and a preservation method of sturgeon. Background technique [0002] Sturgeon's sturgeon belongs to Osteophytes, Atenopterygia, Sclerophyta, and Sturgeoniformes. It is mainly distributed in the Amur River (Heilongjiang, China) basin. Because of its advantages such as fast growth, strong disease resistance, and high economic value, it has been It has become a popular cultured species in China, accounting for 50% of China's total sturgeon production, and there is still an increasing trend. However, due to extensive breeding techniques and high breeding density, the frequency of disease outbreaks has increased, which has greatly hindered the healthy and sustainable development of sturgeon aquaculture. In recent years, scientific researchers have done a lot of work in the protection of Acipenser schneidi resources, artificial breeding, a...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 朱成科吕光俊蒋礼杨成年阳龙江唐征县韩璐璐彭小倩
Owner SOUTHWEST UNIVERSITY
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