NGS-based joint detection system for genetic markers STR and SNP and detection method

A joint detection and genetic marker technology, applied in biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve the problems of expensive kits and unfavorable practical application of forensic science, and achieve accuracy assurance, Accurate and reliable results

Inactive Publication Date: 2021-04-23
河南高新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the kits are expensive, and the supporting data analysis software can only be developed for each kit. The parameter settings are relatively fixed, and only the sequencing data of the inherent loci can be analyzed, which is not conducive to the practical application of forensic science.

Method used

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  • NGS-based joint detection system for genetic markers STR and SNP and detection method
  • NGS-based joint detection system for genetic markers STR and SNP and detection method
  • NGS-based joint detection system for genetic markers STR and SNP and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Evaluating the performance of a NGS-based STR and SNP genetic marker joint detection technology system and detection method for forensic medicine on serially diluted samples

[0068] 1. Sample preparation:

[0069] (1) The FTA blood card samples of 8 unrelated healthy male individuals were taken from the Han population, and the DNA was extracted according to the standard in GA / T383-2014, using the chelex method.

[0070] (2) Repeatability and sensitivity section

[0071] One sample was used to prepare serial dilution series of DNA templates (1ng, 500pg, 200pg, 100pg, 50pg) for sequencing using qubit absolute quantification results, and each sample was tested 3 times in parallel for repeatability and sensitivity studies. In addition, the sequencing data of a standard 2800M Control DNA was analyzed to study the threshold setting.

[0072] 2. Library construction:

[0073] Apply the library construction method described in step S2 of the present invention to c...

Embodiment 2

[0090] Example 2 Evaluating the performance of a NGS-based combined detection technology system and detection method for forensic STR and SNP genetic markers for mixed samples

[0091] 1. Sample preparation (mixed DNA part): According to the quantitative results of Example 1, the DNA quantitative results of 8 male samples were grouped, and 2 DNA samples of single typing were selected to construct 4 groups of DNA samples mixed with two males. Four mixing ratios (1:1, 1:4, 1:9, 1:19) were set for the group mixed samples, and each mixed sample was tested 3 times in parallel.

[0092]2. Library construction: Applying the library construction method in step S2 of the present invention, different mixed samples are used for library construction and sequencing detection with an initial DNA amount of 1 ng. For specific implementation steps, see the implementation method of the library construction link in the specific technical scheme.

[0093] 3. Sequencing and data analysis: Apply th...

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Abstract

The invention belongs to the technical field of biology and particularly relates to a NGS-based joint detection system for genetic markers STR and SNP and a detection method. The selected genetic markers comprise an euchromosome STR, a male-specific Y chromosome STR and a Y chromosome SNP genetic marker, more genetic information can be detected in one time, and a reliable guarantee is provided for accuracy of sequencing data; and the detection system and the detection method have the advantages of good repeatability and high detection performance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an NGS-based combined detection system and detection method for STR and SNP genetic markers. Background technique [0002] Human Y chromosome short tandem repeat (Y-STR) has the characteristics of paternal inheritance, lack of recombination, simple typing, large amount of information, and high polymorphism. powerful tool. At present, family investigation using Y-STR has become an important way of criminal investigation, which can effectively reduce the investigation work in criminal case handling. However, due to the insufficient number of Y-STR sites in the current commercialized kits, the actual number of individuals returning the results is too large, which cannot effectively reduce the workload of criminal investigations. [0003] Currently, fluorescence-labeled multiplex amplification combined with capillary electrophoresis (PCR-CE) is the mainstream technique for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 周亮董晓静夏昊强张振兴全旺
Owner 河南高新生物科技有限公司
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