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Endometrium stem cell exosome gel

A technology of endometrial stem cells and exosomes, applied in the field of biomedicine, can solve the problems of single variety and inconvenient use for patients, and achieve the effects of improving women's health, avoiding ethical issues, and repairing uterine trauma.

Inactive Publication Date: 2021-04-27
焕生汇生物基因技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing varieties are single, and it is inconvenient for patients to use them, so they cannot fully meet the needs of the market.

Method used

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  • Endometrium stem cell exosome gel
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Extraction and cultivation of endometrial stem cells

[0040] The extraction and cultivation of endometrial stem cells include the following steps:

[0041] 1) Collect menstrual blood: centrifuge the menstrual blood at 2000rpm for 10min after balancing, and discard the supernatant;

[0042] 2) If the volume of red cells in the lower layer is less than 5ml, take a 50ml centrifuge tube and add 15ml of lymphocyte separation medium; add an equal volume of normal saline to the red blood cells, mix well, slowly add to the surface of the lymphocyte separation liquid, and centrifuge at 2500rpm for 20min , slowly rising and falling slowly;

[0043] 3) Aspirate the cells in the middle film layer into a new 50ml centrifuge tube, fill with normal saline, 2000rpm, 5min, discard the supernatant;

[0044] 4) Add 10ml primary culture medium to resuspend, inoculate into T75 culture flask, place at 37°C, 5% CO 2 Cultivate in the incubator, observe the growth status of the ce...

Embodiment 2

[0049] An endometrial stem cell exosome gel, comprising: a concentrated solution of traditional Chinese medicine components and endometrial stem cell exosomes with a volume ratio of 1:1, the traditional Chinese medicine ingredients include: 1 gram of mulberry octopus and 0.5 gram of cuttlefish bone , 0.5 grams of Yizhiren, 0.5 grams of black medicine, 0.5 grams of cnidium, 0.1 grams of sea octopus, 1 gram of aloe vera, 0.1 grams of saffron and 0.5 grams of rose hydrosol.

[0050] The extraction method of endometrial stem cell exosomes comprises the following steps:

[0051] 1) Exosomes were extracted by ultrafiltration centrifugation;

[0052] ① The sample is processed by a 0.22um filter, 10ML phosphate buffer (PBS) pre-treated ultrafiltration tube, 4 degrees (°C), 4000g, centrifuged for 10min;

[0053] Ultrafiltration tube inner tube and collection tube to remove phosphate buffer;

[0054] ② Add 15ML sample to the inner tube of the ultrafiltration tube, centrifuge at 4000g ...

Embodiment 3

[0061] Inoculate the mixture of 1ul to 500ul of the purified exosomes and the concentrated solution of traditional Chinese medicine ingredients and the mixture of separate Chinese medicine ingredients with the bacteria solution containing Escherichia coli DH5a respectively. After mixing, smear the blood plate, and incubate in a 37-degree incubator for 16 hours , observe the number of colonies; the results show that the mixture of exosomes and concentrated Chinese medicine ingredients can significantly inhibit the growth of bacteria. The combination of endometrial exosomes and traditional Chinese medicine is effective, see figure 1 shown.

[0062] The specific components and preparation methods of the exosomes and the concentrated solution of traditional Chinese medicine ingredients are the same as in Example 2.

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Abstract

The invention discloses an endometrium stem cell exosome gel, which comprises traditional Chinese medicine ingredients and endometrium stem cell exosome, wherein the traditional Chinese medicine ingredients comprises ootheca mantidis, ossa sepiae, fructus alpiniae oxyphyllae, radix linderae, cnidii fructus, endoconcha sepiae, aloe, stigma croci and rose hydrosols. The endometrium stem cell exosome gel adopts the traditional Chinese medicine ingredients which have higher safety than Western medicines, the traditional Chinese medicines are purely natural, are free from pollution, have the functions of diminishing inflammation and inhibiting bacteria, can improve female health, reduce female gynecological diseases, repair female uterus injuries and recover vaginal elasticity. The endometrium stem cell exosome gel is conveniently used and has good absorbency, the water-soluble gel has good absorbency, and the molecules of the exosome are small so as to be more favorable for absorption; and the endometrium exosome has a good combination effect with the traditional Chinese medicines, carries out convergence and firming on the endometrium, belongs to autologous stem cells and avoids ethical issues, and the autologous stem cells are not be rejected.

Description

technical field [0001] The present application relates to the technical field of biomedicine, in particular to an endometrial stem cell exosome gel. Background technique [0002] Contemporary women have a lot of stress in life, irregular work and rest, go to bed late and wake up late, in a fast-paced era, and eat unhealthy food. Now more and more people suffer from gynecological diseases, tend to be younger, suffer from premature ovarian failure, polycystic ovaries , cancer, and infertility are increasing, so it is particularly important to improve ovarian function for these diseases. [0003] The existing product Sophora flavescens gel is antibacterial and anti-inflammatory. It is a traditional Chinese medicine preparation widely used clinically to treat gynecological inflammation. It is used for chronic gynecological inflammation such as cervical erosion, red and white vaginal discharge, trichomonas vaginitis and vaginal fungal infection. It has good therapeutic effect. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9062A61K9/06A61K47/32A61K47/36A61P15/00A61P15/02C12N5/071A61K35/48A61K35/618A61K35/64
CPCA61K9/06A61K35/48A61K35/618A61K35/64A61K36/234A61K36/54A61K36/738A61K36/88A61K36/886A61K36/896A61K36/9062A61K47/32A61K47/36A61K2236/331A61P15/00A61P15/02C12N5/0682C12N2509/10A61K2300/00
Inventor 卓鑫杰王超君姚玲玲罗保君杨苗孟德龙宋丹
Owner 焕生汇生物基因技术(北京)有限公司
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