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Device and method for synchronously enriching acidic and alkaline DAMO microorganisms

A microbial and acidic technology, applied in the direction of supporting/immobilizing microorganisms, enzymatic/microbiological devices, biological material sampling methods, etc., can solve the unknown contribution of methane oxidation, unknown genetic metabolism mechanism, lack of high-abundance enrichment things and other issues

Pending Publication Date: 2021-04-27
ZHEJIANG UNIV
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  • Claims
  • Application Information

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Problems solved by technology

In recent years, researchers have detected the existence of DAMO microorganisms in both acidic and alkaline environments, but due to the lack of high-abundance enrichments, their environmental adaptation mechanism is unclear, the genetic metabolic mechanism is unknown, and the contribution of methane oxidation is unknown.

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  • Device and method for synchronously enriching acidic and alkaline DAMO microorganisms
  • Device and method for synchronously enriching acidic and alkaline DAMO microorganisms
  • Device and method for synchronously enriching acidic and alkaline DAMO microorganisms

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Embodiment Construction

[0031] The present invention will be further elaborated and illustrated below in conjunction with the accompanying drawings and specific embodiments. The technical features of the various implementations in the present invention can be combined accordingly on the premise that there is no conflict with each other.

[0032] Such as figure 1As shown, a device for simultaneously enriching acidic and alkaline DAMO microorganisms provided by the present invention includes a membrane module 7 and a reactor module. Specifically subdivided, its main structure includes feed system Ⅰ, parallel reactor system Ⅱ, pH control system Ⅲ, temperature control system Ⅳ and water outlet system Ⅴ. The structure and connection mode of each part will be described in detail below.

[0033] The feeding system I includes an argon gas bottle 1 , a water inlet tank 2 , a second gas balance bag 32 , a methane gas bottle 4 , an explosion-proof valve 5 , a pressure sensor 6 , a membrane module 7 and a peri...

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Abstract

The invention discloses a device and a method for synchronously enriching acidic and alkaline DAMO microorganisms. The device comprises a membrane assembly and a reactor module which are connected to each other. The reactor module comprises an acidic reactor and an alkaline reactor which are arranged in parallel and have the same structure. A reaction chamber is internally divided into a settling zone and a reaction zone through a vertical partition plate. A first hole for gas circulation is arranged on the upper part of the partition plate, and a second hole for sludge backflow is arranged on the bottom of the partition plate. The acid reactor is provided with a heating sleeve, a sampling pipe, a stirring device, a gas balance bag, a pH electrode and an adjusting pipe. According to the invention, a specific acidic and alkaline enrichment environment can be provided by utilizing a pH regulation system. The reactor systems are arranged in parallel to realize synchronous enrichment of acidic and alkaline DAMO microorganisms. Organic glass shells of the reactor systems are specifically provided with the settling zone to ensure retention of biomass. A continuous flow full-mixed methane bubbleless aeration enrichment device is used to achieve synchronous enrichment of acidic and alkaline DAMO microorganisms safely and efficiently.

Description

technical field [0001] The invention belongs to the field of enrichment of anaerobic methane oxidation microorganisms, in particular to a device and method for synchronous enrichment of acidic and alkaline DAMO microorganisms. Background technique [0002] Denitrification Anaerobic Methane Oxidation (DAMO) couples denitrification process and methane oxidation process, and can simultaneously remove greenhouse gas methane and polluting substances nitrate nitrogen and nitrite nitrogen under anaerobic conditions, including The process theoretical equations of nitrate-type anaerobic methane oxidation (Na-DAMO) and nitrite-type anaerobic methane oxidation (Ni-DAMO) are as follows: [0003] 5CH 4 +8NO 3 - +8H + →5CO 2 +4N 2 +14H 2 O ΔG 0′ =-765kJ mol -1 [0004] 3CH 4 +8NO 2 - +8H + →3CO 2 +4N 2 +10H 2 O ΔG 0′ =-928kJ mol -1 [0005] Currently known DAMO-enriched cultures include two types: 1) Candidatus Methanoperedens nitroreducens belonging to ANME-2d archaea...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/26C12M1/12C12M1/04C12M1/02C12M1/00
CPCC12M23/58C12M27/02C12M29/04C12M29/06C12M29/26C12M33/04C12M37/04C12M41/40C12M41/26C12M41/22C12M41/12Y02E50/30
Inventor 胡宝兰和明月姚翔午周猛屈莹叶天强郑平
Owner ZHEJIANG UNIV
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