The invention relates to the technical field of
food safety detection, and discloses an immunochromatography kit for rapidly detecting rice
yeast acid in food, which comprises a test strip and an extracting solution. The test strip is in
lap joint with a sample pad, a combination pad, a
nitrocellulose membrane and a water absorption pad in sequence; the
nitrocellulose membrane is provided with a detection line coated with a rice
yeast acid conjugate and a
quality control line coated with an anti-mouse IgG
antibody. The sample pad is pretreated by
polyvinylpyrrolidone to intercept impurities; introducing a steric hindrance protective agent into the combination pad probe; the extracting solution contains
methanol and a surfactant. Through a specific formula and process, the technical problems that in a lipid-containing and high-
viscosity complex matrix, rice
yeast acid is difficult to extract,
chromatography is prone to blockage and false positive is caused by an
organic solvent are solved, and the method has the advantages of being high in sensitivity, high in anti-interference capacity and rapid in detection and is particularly suitable for
food poisoning site emergency disposal and daily rapid screening.