Microarray fluorescent sensing chip and application thereof

A fluorescence sensing and microarray technology, applied in the fields of nanotechnology and food detection, can solve the problems of low accuracy, cumbersome operation, low sensitivity, etc., and achieve the effects of increasing storage time, expanding application range and increasing sensitivity.

Pending Publication Date: 2021-05-07
LUZHOU PINCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the deficiencies of the above-mentioned existing detection technology, the purpose of the present invention is to provide a microarray fluorescent sensor chip and its application, which solves the problem of high cost, cumbersome operation and low sensitivity of the existing detection method, resulting in low accuracy. The problem

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  • Microarray fluorescent sensing chip and application thereof
  • Microarray fluorescent sensing chip and application thereof
  • Microarray fluorescent sensing chip and application thereof

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Embodiment Construction

[0030]Below in conjunction with embodiment the present invention is described in further detail. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0031] 1. Construction of microarray fluorescence array sensor chip

[0032] 1) Synthesis of polystyrene microspheres: 0.95 mL of acrylic acid and 11 mL of styrene were respectively added into a three-necked flask containing 100 mL of ultrapure water, and nitrogen was bubbled for 10 min. With stirring at 350 rpm, the solution was slowly heated to 70 °C and stirred under nitrogen. After reaching the target temperature, ammonium persulfate was quickly added to make the concentration 0.90 mg / mL, and after continuous stirring for 8 hours, the reaction solution was cooled to room temperature, and the complement components were removed to obtain polysty...

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Abstract

The invention discloses a microarray fluorescent sensing chip and application thereof, the chip comprises an array carrier and six fluorescent dyes respectively fixed on the array carrier, the array carrier is a photonic crystal structure formed by self-assembly of polystyrene microspheres on the surface of plasma glass, and the fluorescent dyes are NR, TDS, DR, Rh6G, NED and Np. According to the invention, the photonic crystal is applied to Baijiu detection for the first time, and a solid array is constructed by combining the fluorescence amplification characteristic of the photonic crystal and the broad-spectrum recognition capability of the dye to be applied to detection and recognition of different Baijiu. The method has good identification capability on Baijiu, and an accurate, sensitive and rapid new method is provided for Baijiu detection.

Description

technical field [0001] The invention relates to the technical fields of nanotechnology and food detection, in particular to a microarray fluorescent sensor chip and its application. Background technique [0002] Liquor is mostly composed of ethanol and water, and trace components only account for 2%. Due to the diversity of liquor brewing microorganisms, under the joint action of various microorganisms, there are many flavor components in liquor, and the concentrations are all low, ranging from ng / L to mg / L. At the same time, the content and The proportions are different, thereby forming the different aromas and styles of various liquors. At present, some conventional methods have been used to distinguish liquors of different categories and origins, such as mass spectrometry and spectrometry; although spectrometry is a simple, fast, and nondestructive method, its sensitivity is relatively low, and it is easy to detect in the presence of similar spectra. Interference occurs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6454
Inventor 张宿义沈才洪黄锐乔彩林侯长军霍丹群岳高昂
Owner LUZHOU PINCHUANG TECH CO LTD
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