Microarray fluorescent sensing chip and application thereof
A fluorescence sensing and microarray technology, applied in the fields of nanotechnology and food detection, can solve the problems of low accuracy, cumbersome operation, low sensitivity, etc., and achieve the effects of increasing storage time, expanding application range and increasing sensitivity.
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[0030]Below in conjunction with embodiment the present invention is described in further detail. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0031] 1. Construction of microarray fluorescence array sensor chip
[0032] 1) Synthesis of polystyrene microspheres: 0.95 mL of acrylic acid and 11 mL of styrene were respectively added into a three-necked flask containing 100 mL of ultrapure water, and nitrogen was bubbled for 10 min. With stirring at 350 rpm, the solution was slowly heated to 70 °C and stirred under nitrogen. After reaching the target temperature, ammonium persulfate was quickly added to make the concentration 0.90 mg / mL, and after continuous stirring for 8 hours, the reaction solution was cooled to room temperature, and the complement components were removed to obtain polysty...
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