A method for controlling cabbage clubroot combined with ozone water and Trichoderma
A technology of cabbage clubroot and ozone water, applied in the direction of pest control, chemical instruments and methods, botany equipment and methods, etc., can solve the problems of restricting the popularization and application of ozone, and achieve the effect of promoting the growth of cabbage and improving soil
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Embodiment 1
[0029] Example 1 Determination of the base of Plasmodium brassicae in soil based on qPCR technology
[0030] The serpentine sampling method was used for rectangular plots, and the five-point cross sampling method was used for square plots. Land area less than 670m 2 Take 3~5 points when the time is greater than 670m 2 Take 6~10 points every time, and the sampling depth is about 20cm of the plow layer soil. The collected soil samples were collected and mixed evenly, and the excess soil was discarded by the quartering method, and 200g was retained as a sample. Each plot was re-sampled to obtain 3 samples.
[0031] 0.5 g of soil sample was weighed, and soil DNA was extracted using DNeasy Power Soil DNA Isolation Kit (Qiagen, Valencia, CA) according to the instructions, and the quality and concentration of DNA were detected by 0.8% agarose gel electrophoresis. According to the conserved sequence of the ITS region of Plasmodium brassicae, specific primers PBF (5'-GAACGGGTTCACAG...
Embodiment 2
[0034] Example 2 The inhibitory effect of different concentrations of ozone water on root root bacteria in soil
[0035]Weigh 100 g of fresh cabbage clubroot tissue, add 100 mL of sterile water, and homogenate the tissue at low temperature as the clubroot spore suspension. Measure the suspension, mix it with sterilized soil at a v / w ratio of 5% and 15% respectively to make low-content and high-content diseased soil, and keep samples for testing. The pots used in the test are circular flower pots with a depth of 20 cm and a diameter of 15 cm. Each pot is filled with 2 kg of soil, and is irrigated with ozone water with a concentration of 2 mg / L, 4 mg / L, 6 mg / L, 8 mg / L, and 10 mg / L. Pots were poured with 2L of ozone water, and the same amount of sterile water was poured in the control, and each treatment was repeated three times. After being placed in the sterile culture room for 7 days, 0.5 g of soil samples were mixed in each pot, and the soil DNA was extracted using the DNeas...
Embodiment 3
[0040] Example 3 Effects of Different Concentrations of Ozone Water on Cabbage Germination and Growth
[0041] The soil for potting was collected in the experimental base of the Institute of Ecology, Shandong Academy of Sciences. It is ordinary garden soil. After removing 1-2cm of topsoil, a 0-20cm soil sample was collected, mixed at multiple points and passed through a 50-mesh sieve. The pots used in the test are circular flower pots with a depth of 20 cm and a diameter of 15 cm. Each pot is filled with 2 kg of soil, and is watered with ozone water with a concentration of 2 mg / L, 4 mg / L, 6 mg / L, 8 mg / L, and 10 mg / L. Pots were poured with 2L of ozone water, and the same amount of sterile water was poured in the control. Three replicates were set up for each treatment. Sowing was carried out when the soil moisture was suitable, and routine management was carried out. After 4 weeks, the growth indicators of cabbage were investigated. The results are shown in Table 2.
[0042] Ta...
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